MIR spectroscopy as alternative method for further confirmation of foodborne pathogens Salmonella spp. and Listeria monocytogenes
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Listeriosis and Salmonellosis are two of the most common foodborne diseases. Consequently, an early and accurate detection of Listeria monocytogenes and Salmonella spp. in food products is a critical concern of public health policies. Therefore, it is of great interest to develop rapid, simple, and inexpensive alternatives for pathogen detection in food products. In this study, mid-infrared spectroscopy has been successfully used to confirm Listeria species and the presence of Salmonella isolated from food samples. This methodology showed to be very sensitive and could be a rapid alternative to detect these important pathogens, allowing to obtain results in a few minutes after previous growth in selective media, avoiding the confirmation procedures that delay the achievement of the results for up to 2 days.
KeywordsInfrared spectroscopy (IR) Principal component analysis (PCA) Foodborne bacteria Listeria monocytogenes Salmonella spp
We would like to thank Fundação para a Ciência e a Tecnologia (FCT, Portugal), the European Union, QREN, FEDER, COMPETE, for Funding the Organic Chemistry Research Unit (QOPNA) (Project PEst-C/QUI/UI0062/2013; FCOMP-01-0124-FEDER-037296). Catarina Moreirinha was financed by FCT (SFRH/BD/71512/2010).
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Conflict of interest
The authors declare that they have no conflict of interest.
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