Phenolic profiling and antioxidant capacity in flowers, leaves and peels of Tunisian cultivars of Punica granatum L.
The comprehensive phenolic fingerprints of flowers, peels and leaves of two Tunisian Punica granatum L. cultivars, namely Nabli and Gabsi, were investigated. The highest phenolic content was recorded in the Nabli flowers, followed by Gabsi peels extracts (152.6 and 125.8 mg gallic acid equivalent 100 g−1, respectively) while flavonoids and flavonols were highest in Gabsi peels (19.2 and 26.0 mg Rutin equivalent per 100 g−1). Besides, the antioxidant capacity was the highest in Gabsi peels (144.4 mg gallic acid equivalent 100 g−1) and in Nabli flowers (161.6 mg gallic acid equivalent per 100 g−1. Methanol extracts of all three plant portions of both cultivars were screened by ultra-high-performance liquid chromatography coupled to quadruple time of flight mass spectrometry, and the identified phenolics were further quantified. Nabli cultivar showed higher contents of flavonoids (in flowers and leaves), while phenolic acids were abundant in Gabsi leaves. Multivariate statistics highlighted differences in phenolic profile among organs and cultivars. These results showed that the pomegranate portions investigated are a valuable source of bioactive compounds with health-promoting properties, mainly belonging to the phenolic class of flavonoids.
KeywordsPunica granatum L. Punicaceae Bioactive polyphenols Gabsi Nabli Metabolomics
GR was recipient of a fellowship from the Doctoral School on the Agro-Food System (Agrisystem) of the Università Cattolica del Sacro Cuore (Piacenza, Italy) whereas BF received a traveling fellowship from the University of Tunis El-Manar (Tunis, Tunisia).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Artik N, Murakami H, Mori T (1998) Determination of phenolic compounds in pomegranate juice by HPLC. Fruit Process 12:492–499Google Scholar
- Aviram M, Volkova N, Coleman R, Dreher M, Reddy ML, Ferreira D, Rosenblat M (2008) Phenolics from the peels, arils, and flowers are antiatherogenic: studies in vivo in atherosclerotic apolipoprotein E-deficient (E0) mice and in vitro in cultured macrophages and lipoproteins. J Agric Food Chem 56:1148–1157CrossRefPubMedGoogle Scholar
- El-kar C, Mtimet N, Ferchichi A, Bouajila J (2013) Relationships between fruit acceptability and health-case of seven pomegranate (Punica granatum L.) juices. Food Nutr Sci 4:119–130Google Scholar
- Mars M (2000) Pomegranate plant material: genetic resources and breeding, a review. In: Melgarejo P, Martínez-Nicolás JJ, Martínez-Tomé J (eds) Production, processing and marketing of pomegranate in the Mediterranean region: advances in research and technology. CIHEAM, Zaragoza, pp 55–62 (Options Méditerranéennes: Série A. Séminaires Méditerranéens; n. 42)Google Scholar
- Mekni M, Dhibi M, Kharroubi W, Hmida RB, Cheraif I, Hammami M (2014) Natural conjugated and trans fatty acids in seed oils and phytochemicals in seed extracts issued from three Tunisian pomegranate (Punica granatum L.) cultivars. Int J Curr Microbiol App Sci 3(8):778–792Google Scholar
- Parmar N, Singh N, Kaur A, Thakur S (2017) Comparison of color, anti-nutritional factors, minerals, phenolic profile and protein digestibility between hard-to-cook and easy-to-cook grains from different kidney bean (Phaseolus vulgaris) accessions. J Food Sci Technol 54(4):1023–1034CrossRefPubMedPubMedCentralGoogle Scholar
- Rocchetti G, Chiodelli G, Giuberti G, Ghisoni S, Baccolo G, Blasi F, Montesano D, Trevisan M, Lucini L (2018a) UHPLC-ESI-QTOF-MS profile of polyphenols in Goji berries (Lycium barbarum L.) and its dynamics during in vitro gastrointestinal digestion and fermentation. J Funct Foods 40:564–572CrossRefGoogle Scholar
- Rothwell JA, Pérez-Jiménez J, Neveu V, Medina-Ramon A, M’Hiri N, Garcia-Lobato P, Manach C, Knox C, Eisner R, Wishart DS, Scalbert A (2013) Phenol-Explorer 3.0: a major update of the Phenol-Explorer database to incorporate data on the effects of food processing on polyphenol content. Database. https://doi.org/10.1093/database/bat070 CrossRefPubMedPubMedCentralGoogle Scholar
- Sreekumar S, Sithul H, Muraleedharan P, Azeez JM, Sreeharshan S (2014) Pomegranate fruit as a rich source of biologically active compounds. Biomed Res Int. Article ID 686921Google Scholar
- Worley B, Powers R (2013) Multivariate analysis in metabolomics. Current. Metabolomics 1:92–107Google Scholar
- Yermakov AI, Arasimov VV, Yarosh NP (1987) Methods of biochemical analysis of plants. Agropromizdat, LeningradGoogle Scholar