Extraction and recovery of phytochemical components and antioxidative properties in fruit parts of Dacryodes rostrata influenced by different solvents
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This study investigated the recovery of phytochemical antioxidants in Dacryodes rostrata fruit using different extraction solvents. The effects of solvent of varying polarities with sequential extraction method on the recovery of phenolics, flavonoids, carotenoids and anthocyanins from different parts of the fruit (seed, pulp and peel) were determined. Their antioxidant activities were further determined using DPPH radical, ferric reducing antioxidant power (FRAP), hydroxyl radical scavenging, superoxide anion radical scavenging and phosphomolybdenum method. Dacryodes Rostrata seed had the highest total phenolic content with 50% ethanol as the most efficient extraction solvent. The highest total flavonoid content was obtained in ethyl acetate extract of fruit pulp, whereas peel extracted with hexane and 50% ethanol was the highest in total carotenoid content and total anthocyanin content, respectively. The seed extracted with 50% ethanol exhibited the strongest DPPH radical scavenging activity. Iron chelating activity measured by FRAP assay was the best in seed extracts, particularly in those polar extracts derived from water and 50% ethanol. Antioxidant activities of 50% ethanol extract of D. rostrata seed was the highest when determined by FRAP and phosphomolydenum assays. However, the influence of extraction solvents is not distinctly shown by hydroxyl radical and superoxide anion radical scavenging activities. This is the first report on the effect of various extraction solvents on the recovery of phytochemicals in D. rostrata fruit parts and the seed of D. rostrata is a potential source of polar antioxidants.
KeywordsAnthocyanin Antioxidant Carotenoid Flavonoid Kembayau Polyphenol
We would like to express our gratitude to the technical supports from laboratory staffs of School of Engineering, Monash University Malaysia and the laboratory facilities provided.
- Giusti MM, Wrolstad RE (2005) Characterization and measurement of anthocyanins by UV–visible spectroscopy. In: Wrolstad RE, Acree TE, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker CF, Smith D, Sporns P (eds) Handbook of food analytical chemistry, vol 2-2. Wiley, Hoboken, pp 19–31Google Scholar
- Oliferenko AA, Oliferenko PV, Huddleston JG, Rogers RD, Palyulin VA, Zefirov NS, Katritzky AR (2004) Theoretical scales of hydrogen bond acidity and basicity for application in QSAR/QSPR studies and drug design. Partitioning of aliphatic compounds. J Chem Inform Comput Sci 44(3):1042–1055CrossRefGoogle Scholar
- Prasad N, Yang B, Kong KW, Khoo HE, Sun J, Azlan A, Ismail A Romli ZB (2013) Phytochemicals and antioxidant capacity from Nypa fruticans Wurmb. fruit. Evid Based Complement Alternat Med Article ID 154606, 9 pagesGoogle Scholar
- Tee LH, Ramanan RN, Tey BT, Chan ES, Azrina A, Amin I, Yang B, Lau CY, Prasad KN (2015) Phytochemicals and antioxidant capacities from Dacryodes rostrata fruits. Med Chem 5:023–027Google Scholar
- Tomsone L, Kruma Z (2013) Comparison of different solvents for isolation of phenolic compounds from horseradish (Armoracia rusticana L.) leaves. In: Treija S, Skujeniece S (eds) Research for rural development. Latvia University of Agriculture, Jelgava, pp 104–110Google Scholar