A simple assay platform for sensitive detection of Sudan I–IV in chilli powder based on CsPbBr3 quantum dots
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Sudan dyes are phenyl-azoic derivatives widely used in industry. Classified as carcinogenic and are strictly forbidden in foodstuffs; however, some unscrupulous businessmen adopted it for coloring foodstuffs. Here, a simple and effective fluorescence (FL) assay platform has been developed for the detection of Sudan I–IV based on CsPbBr3 perovskite quantum dots (QDs). It was found that the fluorescent emission of CsPbBr3 QDs can be effectively quenched by Sudan I–IV. Under the optimized conditions, the FL quenching efficiency of CsPbBr3 QDs was quantitatively correlated to the logarithmic concentrations of Sudan I–IV over the ranges of 100–10,000, 0.1–1000, 0.1–2000 and 0.4–1000 ng mL−1 for Sudan I–IV, and the corresponding limits of detection were 3.33, 0.03, 0.03 and 0.04 ng mL−1 (at 3σ/slope), respectively. CsPbX3 QDs (X = Cl, Br, and I or mixed halide systems Cl/Br and Br/I) was utilized as sensor in FL assay, which have unique optical properties of high FL quantum yields (up to 90%), narrow half peak width (26 nm) and tunable FL emissions spectra (410–700 nm). Meanwhile, the practical use of this assay platform for Sudan I–IV detection in chilli powder samples was also demonstrated, which indicated the potential in practical applications.
KeywordsChili powder Sudan I–IV CsPbBr3 QDs FL assay platform detection
This work was supported by the National Natural Science Foundation of China (21475043 and 21675051), and The Foundation of the Science and Technology Department of Hunan Province (2016SK2020).
- Mazzotti F, Di Donna L, Maiuolo L, Napoli A, Salerno R, Sajjad A, Sindona G (2008) Assay of the set of all Sudan azodye (I, II, III, IV, and para-red) contaminating agents by liquid chromatography–tandem mass spectrometry and isotope dilution methodology. J Agric Food Chem 56:63–67CrossRefGoogle Scholar
- Protesescu L, Yakunin S, Bodnarchuk MI, Krieg F, Caputo R, Hendon CH, Yang RX, Walsh A, Kovalenko MV (2015) Nanocrystals of cesium lead halide perovskites (CsPbX3, X = Cl, Br, and I): novel optoelectronic materials showing bright emission with wide color gamut. Nano Lett 15:3692–3696CrossRefGoogle Scholar
- Stiborová M, Martínek V, Rýdlová H, Hodek P, Frei E (2002) Sudan I is a potential carcinogen for humans: evidence for its metabolic activation and detoxication by human recombinant cytochrome P450 1A1 and liver microsomes. Cancer Res 62:5678–5684Google Scholar
- Xiao Z, Loughlin F, George GN, Howlett GJ, Wedd AG (2004) C-terminal domain of the membrane copper transporter Ctr1 from Saccharomyces cerevisiae binds four Cu(I) ions as a cuprous-thiolate polynuclear cluster: sub-femtomolar Cu(I) affinity of three proteins involved in copper trafficking. J Am Chem Soc 126:3081–3090CrossRefGoogle Scholar