Journal of Food Science and Technology

, Volume 55, Issue 7, pp 2467–2475 | Cite as

Combined postharvest UV-C and 1-methylcyclopropene (1-MCP) treatment, followed by storage continuously in low level of ethylene atmosphere improves the quality of Tahitian limes

  • Penta PristijonoEmail author
  • Michael C. Bowyer
  • Christopher J. Scarlett
  • Quan V. Vuong
  • Costas E. Stathopoulos
  • John B. Golding
Original Article


The green Tahitian limes (Citrus latifolia) were exposed to 7.2 kJ m−2 UV-C and 0.5 μL L−1 1-methylcyclopropene (1-MCP) treatments both separately and in combination. After treatment, fruit were stored in ethylene free (i.e. air containing < 0.005 μL L−1) or 0.1 μL L−1 ethylene at 20 °C and 100% RH. The results showed that UV-C treatment delayed skin degreening and reduced endogenous ethylene production compared to untreated control fruit, however these effects reduced over the storage time. As expected, 1-MCP inhibited ethylene production, reduced calyx abscission and retained peel greenness during the storage. Both of the combination treatments, 1-MCP + UV-C and UV-C + 1-MCP reduced endogenous ethylene production and delayed skin yellowing. In all treatments, UV-C and 1-MCP resulted in lower fruit respiration rates than untreated control fruit, however this effect diminished during 7 and 14 days storage for fruits stored in air and 0.1 μL L−1 ethylene atmosphere, respectively. There was no difference in weight loss, SSC, TA and SSC/TA ratio between the treatments and storage conditions. The results suggest that a pre-storage UV-C treatment, followed by storage at low level of ethylene improves the quality of limes, with the additional improvement when combined with 1-MCP treatment prior or after UV-C irradiation.


Citrus latifolia Quality Ethylene Respiration Colour Calyx abscission 



This work was supported by the University of Newcastle, Australian Research Council Training Centre for Food and Beverage Supply Chain Optimisation (IC140100032) and NSW Department of Primary Industries. We acknowledge to Agrofresh Solution Inc. for providing 1-MCP for this study.


  1. Barros HR, Ferreira TA, Genovese MI (2012) Antioxidant capacity and mineral content of pulp and peel from commercial cultivars of citrus from Brazil. Food Chem 134:1892–1898. CrossRefPubMedGoogle Scholar
  2. Barsan C, Sanchez-Bel P, Rombaldi C, Egea I, Rossignol M, Kuntz M, Zouine M, Latché A, Bouzayen M, Pech JC (2010) Characteristics of the tomato chromoplast revealed by proteomic analysis. J Exp Bot 61:2413–2431. CrossRefPubMedGoogle Scholar
  3. Buchert AM, Civello PM, Martinez GA (2011) Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets. Sci Hortic 127:214–219. CrossRefGoogle Scholar
  4. Burns JK (2016) Lime. In: Gross NC, CWang CY, Salveit M (eds) The commercial storage of fruits, vegetables, and florist and nursery stocks. USDA, ARS, Washington, DC, pp 390–391Google Scholar
  5. Cantos E, Espin JC, Tomas-Barberan FA (2002) Postharvest stilbene-enrichment of red and white table grape varieties using UV-C irradiation pulses. J Agric Food Chem 50:6322–6329. CrossRefPubMedGoogle Scholar
  6. Charles MT, Arul J, Charlebois D, Yaganza ES, Rolland D, Roussel D, Merisier MJ (2016) Postharvest UV-C treatment of tomato fruits: changes in simple sugars and organic acids contents during storage. Lwt-Food Sci Technol 65:557–564. CrossRefGoogle Scholar
  7. Costa L, Vicente AR, Civello PM, Chaves AR, Martinez GA (2006) UV-C treatment delays postharvest senescence in broccoli florets. Postharvest Biol Technol 39:204–210. CrossRefGoogle Scholar
  8. D’hallewin G, Schirra M, Manueddu E, Piga A, Ben-Yehoshua S (1999) Scoparone and scopoletin accumulation and ultraviolet-C induced resistance to postharvest decay in oranges as influenced by harvest date. J Am Soc Hortic Sci 124:702–707Google Scholar
  9. D’Hallewin G, Schirra M, Pala M, Ben-Yehoshua S (2000) Ultraviolet C irradiation at 0.5 kJ m2 reduces decay without causing damage or affecting postharvest quality of star ruby grapefruit (C. paradisi Macf.). J Agric Food Chem 48:4571–4575. CrossRefPubMedGoogle Scholar
  10. Dou H, Jones S, Ritenour M (2005) Influence of 1-MCP application and concentration on post-harvest peel disorders and incidence of decay in citrus fruit. J Hortic Sci Biotechnol 80:786–792. CrossRefGoogle Scholar
  11. Eaks IL (1970) Respiratory response, ethylene production, and response to ethylene of citrus fruit during ontogeny. Plant Physiol 45:334–338CrossRefPubMedPubMedCentralGoogle Scholar
  12. Goldschmidt EE (1998) Ripening of citrus and other non-climacteric fruits: a role for ethylene. Acta Hortic 463:335–340. CrossRefGoogle Scholar
  13. Gómez-Lobato ME, Hasperué JH, Civello PM, Chaves AR, Martínez GA (2012) Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.). Sci Hortic 144:208–211. CrossRefGoogle Scholar
  14. Gonzalez-Aguilar GA, Zavaleta-Gatica R, Tiznado-Hernandez ME (2007) Improving postharvest quality of mango ‘Haden’ by UV-C treatment. Postharvest Biol Technol 45:108–116. CrossRefGoogle Scholar
  15. Kaewsuksaeng S, Urano Y, Aiamla-or S, Shigyo M, Yamauchi N (2011) Effect of UV-B irradiation on chlorophyll-degrading enzyme activities and postharvest quality in stored lime (Citrus latifolia Tan.) fruit. Postharvest Biol Technol 61:124–130. CrossRefGoogle Scholar
  16. Kaewsuksaeng S, Tatmala N, Srilaong V, Pongprasert N (2015) Postharvest heat treatment delays chlorophyll degradation and maintains quality in Thai lime (Citrus aurantifolia Swingle cv. Paan) fruit. Postharvest Biol Technol 100:1–7. CrossRefGoogle Scholar
  17. Kahlau S, Bock R (2008) Plastid transcriptomics and translatomics of tomato fruit development and chloroplast-to-chromoplast differentiation: chromoplast gene expression largely serves the production of a single protein. Plant Cell 20:856–874. CrossRefPubMedPubMedCentralGoogle Scholar
  18. Kluge RA, Jomori MLL, Jacomino AP, Vitti MCD, Padula M (2003) Intermittent warming in ‘Tahiti’ lime treated with an ethylene inhibitor. Postharvest Biol Technol 29:195–203. CrossRefGoogle Scholar
  19. Liu CH, Cai LY, Lu XY, Han XX, Ying TJ (2012) Effect of postharvest UV-C irradiation on phenolic compound content and antioxidant activity of tomato fruit during storage. J Integr Agric 11:159–165. CrossRefGoogle Scholar
  20. Porat R, Weiss B, Cohen L, Daus A, Goren R, Droby S (1999) Effects of ethylene and 1-methylcyclopropene on the postharvest qualities of ‘Shamouti’ oranges. Postharvest Biol Technol 15:155–163. CrossRefGoogle Scholar
  21. Pristijono P (2007) Use of nitric oxide to extend the postharvest life of fresh-cut and intact fruits and vegetables. (PhD)The University of Newcastle, Australia, 223ppGoogle Scholar
  22. Reid SM (2002) Ethylene in postharvest technology (Oakland, CA: postharvest technology of horticultural crops, regents of the University of California, Division of Agricultural and Natural Resources)Google Scholar
  23. Salvador A, Carvalho CP, Monterde A, Martinez-Javega JM (2006) Note. 1-MCP effect on chilling injury development in ‘Nova’ and ‘Ortanique’ mandarins. Food Sci Technol Int 12:165–170. CrossRefGoogle Scholar
  24. Srilaong V, Aiamla-or S, Soontornwat A, Shigyo M, Yamauchi N (2011) UV-B irradiation retards chlorophyll degradation in lime (Citrus latifolia Tan.) fruit. Postharvest Biol Technol 59:110–112. CrossRefGoogle Scholar
  25. Wang J, You Y, Chen W, Xu Q, Wang J, Liu Y, Song L, Wu J (2015) Optimal hypobaric treatment delays ripening of honey peach fruit via increasing endogenous energy status and enhancing antioxidant defence systems during storage. Postharvest Biol Technol 101:1–9. CrossRefGoogle Scholar
  26. Wills RBH, Warton MA, Ku VVV (2000) Ethylene levels associated with fruit and vegetables during marketing. Aust J Exp Agric 40:465–470CrossRefGoogle Scholar
  27. Win TO, Srilaong V, Heyes J, Kyu KL, Kanlayanarat S (2006) Effects of different concentrations of 1-MCP on the yellowing of West Indian lime (Citrus aurantifolia, Swingle) fruit. Postharvest Biol Technol 42:23–30. CrossRefGoogle Scholar
  28. Xu F, Wang H, Tang Y, Dong S, Qiao X, Chen X, Zheng Y (2016) Effect of 1-methylcyclopropene on senescence and sugar metabolism in harvested broccoli florets. Postharvest Biol Technol 116:45–49. CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2018

Authors and Affiliations

  • Penta Pristijono
    • 1
    Email author
  • Michael C. Bowyer
    • 1
  • Christopher J. Scarlett
    • 1
  • Quan V. Vuong
    • 1
  • Costas E. Stathopoulos
    • 2
  • John B. Golding
    • 1
    • 3
  1. 1.School of Environmental and Life SciencesUniversity of NewcastleOurimbahAustralia
  2. 2.School of Science, Engineering and TechnologyUniversity of AbertayDundeeUK
  3. 3.NSW Department of Primary IndustriesGosfordAustralia

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