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Journal of Food Science and Technology

, Volume 55, Issue 6, pp 2087–2094 | Cite as

Enhanced exopolysaccharide production and biofilm forming ability in methicillin resistant Staphylococcus sciuri isolated from dairy in response to acyl homoserine lactone (AHL)

  • Milind Mohan Naik
  • Shivangi P. Naik
  • Santosh Kumar Dubey
  • Chinmay Bhat
  • Lakshangy S. Charya
Original Article
  • 172 Downloads

Abstract

Staphylococcus sciuri is an emerging human pathogen widely found in dairy industries. In this study, we have isolated methicillin resistant Staphylococcus sp. from biofilm formed on utensil used in the dairy society situated at Raia, Goa and was designated as NN14. The isolate NN14 was identified through 16S rRNA sequencing as S. sciuri (GenBank accession number MF621976). This report reveals that the S. sciuri strain NN14 responds positively to the, acyl-homoserine lactone (AHL) having 6-carbon long acyl chain i.e. N-hexanoyl-homoserine lactone molecule (C6-HSL) with gradual rise in their biofilm establishing potential as the concentration of AHL was increased from 250 nM, 500 nM to 1 µM when compared to control (without C6-HSL) by performing crystal violet assay using 48 well microtiter plate. Also, exopolysaccharide (EPS) production was found to increase with gradual increase in C6-HSL concentration from 250 nM, 500 nM to 1 µM proving potential role of EPS in biofilm formation. These results were further proved by scanning electron microscopy where increased in biofilm and EPS production with increase in C6-HSL concentration was observed. The biofilm forming capability of S. sciuri strain NN14 was found to decreased significantly when it was subjected to 10 µg/ml of (R)-2-(2-hydroxynaphthalen-1-yl)-thiazolidine-4-carboxylic acid, however with the addition of 250 and 500 nM, C6-HSL in presence of the antimicrobial compound (R)-2-(2-hydroxynaphthalen-1-yl)-thiazolidine-4-carboxylic acid, the biofilm development in bacterial strain NN14 was increased when compared with control. Our results demonstrated that the C6-HSL molecule neutralize the effect of antibacterial compound and enhances EPS production and biofilm development in S. sciuri.

Keywords

Staphylococcus sciuri Biofilm Acyl homoserine lactone Antimicrobial compound EPS 

Notes

Acknowledgements

SERB-DST Young Scientist Project (File Number: YSS/2014/000258). Thanks to Ms. Purva Bhangui and Dr. Shyamalina Haldar, Goa University.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

13197_2018_3123_MOESM1_ESM.docx (570 kb)
Supplementary material 1 (DOCX 570 kb)

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Copyright information

© Association of Food Scientists & Technologists (India) 2018

Authors and Affiliations

  • Milind Mohan Naik
    • 1
  • Shivangi P. Naik
    • 1
  • Santosh Kumar Dubey
    • 1
    • 4
  • Chinmay Bhat
    • 2
    • 3
  • Lakshangy S. Charya
    • 1
  1. 1.Department of MicrobiologyGoa UniversityTaleigao PlateauIndia
  2. 2.Department of ChemistryGoa UniversityGoaIndia
  3. 3.Government First Grade CollegeChamarajanagarIndia
  4. 4.Department of BotanyBanaras Hindu UniversityVaranasiIndia

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