Journal of Food Science and Technology

, Volume 55, Issue 2, pp 479–488 | Cite as

Changes in nutritional and bio-functional compounds and antioxidant capacity during black garlic processing

  • Yue-E Sun
  • Weidong Wang
Original Article


This study aimed to explore ideal processing condition for black garlic based on the change of nutritional and active components and antioxidant capacity. Fresh garlic was processed under the condition of constant temperature (65, 75 and 85 °C) and relative humidity (70, 75, 80 and 85%) for 16 days. The sensory scores, contents of nutritional and active components, and antioxidant capacity were monitored. The sensory scores reached the maximum on the 8th day at 85% humidity and 75 °C. The contents of nutritional components were significantly affected by humidity and temperature, and 85% humidity and 75 °C were appropriate. The polyphenol content increased with increase in temperature and decrease in humidity. The reducing sugars and total sugars, total acids and 5-HMF were higher at 75 °C than at 65 and 85 °C. Reducing sugar and protein contents and sensory scores decreased on the 8th day. Maintaining the temperature of 75 °C and relative humidity of 85% for 8 days were ideal for black garlic to retain antioxidant capacity and abundant nutrients.


Black garlic Nutritional components Allicin Thiosulfinates Polyphenols Antioxidant capacity 



This work was supported by National Natural Science Foundation of China (31301535), Qing Lan Project and Xuzhou science and technology plan (KC14NO069).


  1. Ak T, Gülçin İ (2008) Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 174:27–37CrossRefGoogle Scholar
  2. Arts MJ, Dallinga JS, Voss H-P, Haenen GR, Bast A (2004) A new approach to assess the total antioxidant capacity using the TEAC assay. Food Chem 88:567–570CrossRefGoogle Scholar
  3. Bae SE, Cho SY, Won YD, Lee SH, Park HJ (2014) Changes in S-allyl cysteine contents and physicochemical properties of black garlic during heat treatment. LWT Food Sci Technol 55:397–402CrossRefGoogle Scholar
  4. Bozkurt H, Göğüş F, Eren S (1999) Nonenzymic browning reactions in boiled grape juice and its models during storage. Food Chem 64:89–93CrossRefGoogle Scholar
  5. Chung LY (2006) The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin, and allyl disulfide. J Med Food 9:205–213CrossRefGoogle Scholar
  6. Delgado-Andrade C, Seiquer I, Haro A, Castellano R, Navarro MP (2010) Development of the Maillard reaction in foods cooked by different techniques. Intake of Maillard-derived compounds. Food Chem 122:145–153CrossRefGoogle Scholar
  7. Garzón G, Wrolstad R (2009) Major anthocyanins and antioxidant activity of Nasturtium flowers (Tropaeolum majus). Food Chem 114:44–49CrossRefGoogle Scholar
  8. Han J, Lawson L, Han G, Han P (1995) Spectrophotometric method for quantitative determination of allicin and total garlic thiosulfinates. Anal Biochem 225:157–160CrossRefGoogle Scholar
  9. Kwon O-C, Woo K-S, Kim T-M, Kim D-J, Hong J-T, Jeong H-S (2006) Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol 38:331–336Google Scholar
  10. Lan X, Liu P, Xia S, Jia C, Mukunzi D, Zhang X, Xia W, Tian H, Xiao Z (2010) Temperature effect on the non-volatile compounds of Maillard reaction products derived from xylose–soybean peptide system: further insights into thermal degradation and cross-linking. Food Chem 120:967–972CrossRefGoogle Scholar
  11. Ledl F, Schleicher E (1990) New aspects of the Maillard reaction in foods and in the human body. Angew Chem Int Ed Engl 29:565–594CrossRefGoogle Scholar
  12. Liang T, Wei F, Lu Y, Kodani Y, Nakada M, Miyakawa T, Tanokura M (2015) Comprehensive NMR analysis of compositional changes of black garlic during thermal processing. J Agric Food Chem 63:683–691CrossRefGoogle Scholar
  13. Liu H, Zhang J (2008) Antioxidant activity of carboxymethylated and sulfated polysaccharides from pumpkin [J]. Sheng Wu Jia Gong Guo Cheng 4:009Google Scholar
  14. Okada Y, Tanaka K, Fujita I, Sato E, Okajima H (2005) Antiodidant activity of thiosulfinates derived from garlic. Redox Rep 10:96–102CrossRefGoogle Scholar
  15. Purev U, Chung MJ, Oh D-H (2012) Individual differences on immunostimulatory activity of raw and black garlic extract in human primary immune cells. Immunopharmacol Immunotoxicol 34:651–660CrossRefGoogle Scholar
  16. Qingming Y, Xianhui P, Weibao K, Hong Y, Yidan S, Li Z, Yanan Z, Yuling Y, Lan D, Guoan L (2010) Antioxidant activities of malt extract from barley (Hordeum vulgare L.) toward various oxidative stress in vitro and in vivo. Food Chem 118:84–89CrossRefGoogle Scholar
  17. Queiroz YS, Ishimoto EY, Bastos DH, Sampaio GR, Torres EA (2009) Garlic (Allium sativum L.) and ready-to-eat garlic products: in vitro antioxidant activity. Food Chem 115:371–374CrossRefGoogle Scholar
  18. Salman H, Bergman M, Bessler H, Punsky I, Djaldetti M (1999) Effect of a garlic derivative (alliin) on peripheral blood cell immune responses. Int J Immunopharmacol 21:589–597CrossRefGoogle Scholar
  19. Sasaki JI, Lu C, Machiya E, Tanahashi M, Hamada K (2007) Processed black garlic (Allium sativum) extracts enhance anti-tumor potency against mouse tumors. Energy (kcal/100 g) 227:138Google Scholar
  20. van den Berg R, Haenen GR, van den Berg H, Bast A (1999) Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem 66:511–517CrossRefGoogle Scholar
  21. Wang D, Feng Y, Liu J, Yan J, Wang M, Sasaki J-i LuC (2010) Black garlic (Allium sativum) extracts enhance the immune system. Med Aromat Plant Sci Biotechnol 4:37–40Google Scholar
  22. Wang X, Jiao F, Wang Q-W, Wang J, Yang K, Hu R-R, Liu H-C, Wang H-Y, Wang Y-S (2012) Aged black garlic extract induces inhibition of gastric cancer cell growth in vitro and in vivo. Mol Med Rep 5:66–72CrossRefGoogle Scholar
  23. Wootton-Beard PC, Moran A, Ryan L (2011) Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and Folin-Ciocalteu methods. Food Res Int 44:217–224CrossRefGoogle Scholar
  24. Xiao H, Parkin KL (2002) Antioxidant functions of selected allium thiosulfinates and S-alk (en) yl-l-cysteine sulfoxides. J Agric Food Chem 50:2488–2493CrossRefGoogle Scholar
  25. Zhang Z, Lei M, Liu R, Gao Y, Xu M, Zhang M (2015) Evaluation of alliin, saccharide contents and antioxidant activities of black garlic during thermal processing. J Food Biochem 39:39–47CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2017

Authors and Affiliations

  1. 1.College of Food EngineeringXuzhou University of TechnologyXuzhouChina

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