Evaluation of three pumpkin species: correlation with physicochemical, antioxidant properties and classification using SPME-GC–MS and E-nose methods
Abstract
To ascertain the most discriminant variables for three pumpkin species principal component analysis (PCA) was performed. Twenty-four parameters (pH, conductivity, sucrose, glucose, total soluble solids, L*, a*, b*, individual weight, edible rate, firmness, citric acid, fumaric acid, l-ascorbic acid, malic acid, PPO activity, POD activity, total flavonoids, vitamin E, total phenolics, DPPH, FRAP, β-carotene, and aroma) were considered. The studied pumpkin species were Cucurbita maxima, Cucurbita moschata, and Cucurbita pepo. Three pumpkin species were classified by PCA based on aroma, physicochemical and antioxidant properties because the sum of PC1 and PC2 were both greater than 85% (85.06 and 93.64% respectively). Results were validated by the PCA and showed that PPO activity, total flavonoid, sucrose, glucose, TSS, a*, pH, malic acid, vitamin E, DPPH, FRAP and β-carotene, and aroma are highly useful parameters to classify pumpkin species.
Keywords
Antioxidant capacity E-nose PCA Physicochemical PumpkinAbbreviations
- DPPH
1,1-Diphenyl-2-picrylhydrazyl
- E-nose
Electronic nose
- FRAP
Ferric reducing antioxidant power
- FW
Fresh weight
- SPME-GC–MS
Solid phase micro-extraction-gas chromatography–mass spectrometry
- TSS
Total soluble solids
- PCA
Principal component analysis
- POD
Peroxidase
- PPO
Polyphenol oxidase
Notes
Acknowledgements
This work was supported by the Natural Science Foundation of Jiangxi Province, China (Grant No. 20151BAB204039), Doctoral Research Startup Fund Project of Jiangxi Science and Technology Normal University (3000990310), and Quality engineering projects of Jiangxi province (Biological chemistry curriculums, 2015).
References
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