LC–ESI–MS/MS based characterization of phenolic components in fruits of two species of Solanaceae
- 284 Downloads
The present work appraises the phenolic profile of fruit extracts from two species (Solanum indicum and Solanum surattense) of Solanaceae. The bioactive components from both the species were extracted with different concentrations of acidified (hydrolysis) and aqueous methanol (non-hydrolyzed) and analyzed for the total phenolics calorimetrically. The hydrolyzed extracts of both the species were characterized for phenolic compounds profiling using LC–ESI–MS/MS. The hydrolysis of fruit samples produced greater extract yield (31.9–50.7%) and total phenolics (250.4–289.5 mg GAE/g of extract) than non-hydrolyzed (11.1–19.2 and 161.5–171.2, respectively). The hydrolyzed extracts from both the species, with higher contents of total phenols were analyzed for phenolic profiling using LC–ESI–MS/MS technique and the data authenticated the presence of caftaric acid, ellagic acid, myricetin, apigenin-7-glucoside and methoxy chrysin in S. indicum while isoquercitrin, gallocatechin, quercetin and catechin in S. surattense. The current study explores the profile of valuable phenolic bioactives of the selected species of Solanaceae, thereby supporting their potential applications as ingredients of functional foods and neutraceuticals.
KeywordsAcidified methanol Hydrolysis Bioactives extraction Folin reagent Phenolic compounds LC–ESI–MS/MS
Authors extend sincere thanks to Higher Education Commission, Islamabad, Pakistan for providing funding through Indigenous PhD Fellowship Program for the accomplishment of this research work.
- Carazzone C, Mascherpa D, Gazzani G, Papetti A (2013) Identification of phenolic constituents in red chicory salads (Cichorium intybus) by high-performance liquid chromatography with diode array detection and electrospray ionisation tandem mass spectrometry. Food Chem 138:1062–1071CrossRefPubMedGoogle Scholar
- Deb PK, Das L, Ghosh R, Debnath R, Bhakta T (2013) Evaluation of laxative and cardiotonic activity of solanum indicum Linn. fruits. J Pharm Phytoth 1:11–14Google Scholar
- Kajdzanoska M, Gjamovski V, Stefova M (2010) HPLC-DAD-ESI-MSn identification of phenolic compounds in cultivated strawberries from Macedonia. Maced J Chem Chem Eng 29:181–194Google Scholar
- Rispail N, Morris P, Webb KJ (2005) Phenolic compounds: Extraction and analysis. In: Márquez AJ (ed) Lotus Japonicus Handbook, 1st ed. Springer, Dordrecht, The Netherlands, pp 349–354Google Scholar
- Sroka Z, Kuta I, Cisowski W, Drys A (2004) Antiradical activity of hydrolyzed and non-hydrolyzed extracts from Helichrysi inflorescentia and its phenolic contents. Everl Inflores Consti Antirad Prop 59c:363–367Google Scholar
- Theerasin S, Baker A (2009) Analysis and identification of phenolic compounds in Dioscorea hispida Dennst. Asian J Food Agro Ind 2:547–560Google Scholar
- Yadav R, Rathi M, Pednekar A, Rewachandani Y (2016) A detailed review on Solanaceae family. Euro J Pharm Med Res 3(1):369–378Google Scholar