LC–ESI–MS/MS based characterization of phenolic components in fruits of two species of Solanaceae
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The present work appraises the phenolic profile of fruit extracts from two species (Solanum indicum and Solanum surattense) of Solanaceae. The bioactive components from both the species were extracted with different concentrations of acidified (hydrolysis) and aqueous methanol (non-hydrolyzed) and analyzed for the total phenolics calorimetrically. The hydrolyzed extracts of both the species were characterized for phenolic compounds profiling using LC–ESI–MS/MS. The hydrolysis of fruit samples produced greater extract yield (31.9–50.7%) and total phenolics (250.4–289.5 mg GAE/g of extract) than non-hydrolyzed (11.1–19.2 and 161.5–171.2, respectively). The hydrolyzed extracts from both the species, with higher contents of total phenols were analyzed for phenolic profiling using LC–ESI–MS/MS technique and the data authenticated the presence of caftaric acid, ellagic acid, myricetin, apigenin-7-glucoside and methoxy chrysin in S. indicum while isoquercitrin, gallocatechin, quercetin and catechin in S. surattense. The current study explores the profile of valuable phenolic bioactives of the selected species of Solanaceae, thereby supporting their potential applications as ingredients of functional foods and neutraceuticals.
KeywordsAcidified methanol Hydrolysis Bioactives extraction Folin reagent Phenolic compounds LC–ESI–MS/MS
Authors extend sincere thanks to Higher Education Commission, Islamabad, Pakistan for providing funding through Indigenous PhD Fellowship Program for the accomplishment of this research work.
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