Journal of Food Science and Technology

, Volume 53, Issue 9, pp 3408–3423 | Cite as

Plant-based milk alternatives an emerging segment of functional beverages: a review

  • Swati SethiEmail author
  • S. K. Tyagi
  • Rahul K. Anurag
Original Article


Plant-based or non-dairy milk alternative is the fast growing segment in newer food product development category of functional and specialty beverage across the globe. Nowadays, cow milk allergy, lactose intolerance, calorie concern and prevalence of hypercholesterolemia, more preference to vegan diets has influenced consumers towards choosing cow milk alternatives. Plant-based milk alternatives are a rising trend, which can serve as an inexpensive alternate to poor economic group of developing countries and in places, where cow’s milk supply is insufficient. Though numerous types of innovative food beverages from plant sources are being exploited for cow milk alternative, many of these faces some/any type of technological issues; either related to processing or preservation. Majority of these milk alternatives lack nutritional balance when compared to bovine milk, however they contain functionally active components with health promoting properties which attracts health conscious consumers. In case of legume based milk alternatives, sensory acceptability is a major limiting factor for its wide popularity. New and advanced non-thermal processing technologies such as ultra high temperature treatment, ultra high pressure homogenization, pulsed electric field processing are being researched for tackling the problems related to increase of shelf life, emulsion stability, nutritional completeness and sensory acceptability of the final product. Concerted research efforts are required in coming years in functional beverages segment to prepare tailor-made newer products which are palatable as well as nutritionally adequate.


Plant-based milk alternatives Lactose intolerance Non-thermal technologies Emulsion stability Shelf life Fortification 



This manuscript was prepared under the research activity on “oat milk and sesame milk processing” and financial support, facilities received from Indian Council of Agricultural Research (ICAR) and Central Institute of Post-Harvest Engineering and Technology (CIPHET) is gratefully acknowledged.


  1. Alejandro JDA (2007) Method for preparing a beanless-flavor soy milk and/okara using carbon dioxide in a state of sublimation. US7258889B2. 21.08.2007Google Scholar
  2. Badenhop AF, Hackler LR (1970) Effect of soaking soybeans in sodium hydroxide solution as pretreatment for soy milk production. Cereal Sci Today 15(3):84–88Google Scholar
  3. Bastıoğlu AZ, Tomruk D, Koç M, Ertekin FK (2016) Spray dried melon seed milk powder: physical, rheological and sensory properties. J Food Sci Technol 53(5):2396–2404CrossRefGoogle Scholar
  4. Belewu MA, Belewu KY (2007) Comparative physicochemical evaluation of tiger nut, soybean and coconut milk sources. Int J Agric Biol 9(5):785–787Google Scholar
  5. Berger J, Bravay G, Berger M (1997) Almond milk preparation process and products obtained. US5656321 A. 12.08.1997Google Scholar
  6. Biswas S, Sircar D, Mitra A, De B (2011) Phenolic constituents and antioxidant properties of some varieties of Indian rice. Nutr Food Sci 41(2):123–135CrossRefGoogle Scholar
  7. Burton GW, Ingold KU (1989) Vitamin E as an in vitro and in vivo antioxidant. Ann N Y Acad Sci 570:7–22CrossRefGoogle Scholar
  8. Camacho L, Sierra C (1988) Lupin processing for the obtainment of a tofu-like product. In: Twardowski (ed) Proceedings of the 5th international lupin conference. International Lupin Association, Poznan, pp 682–685Google Scholar
  9. Chaiwanon P, Puwastien P, Nitithamyong A, Sirichakwal PP (2000) Calcium fortification in soybean milk and in vitro bioavailability. J Food Compos Anal 13(4):319–327CrossRefGoogle Scholar
  10. Chiba H, Takahashi N, Sasaki R (1979) Enzymatic improvement of food flavor II. Removal of Beany flavor from soybean products by aldehyde dehydrogenase. Agric Biol Chem 43(9):1883–1889Google Scholar
  11. Civille GV, Szczesniak AS (1973) Guidelines to training a texture profile panel. J Texture Stud 4:204CrossRefGoogle Scholar
  12. Codex Alimentarius Commission (1994) Food for special dietary uses (including foods for infants and children), 2nd edn, vol 4. Joint FAO/WHO Food Standards Programme Codex Alimentarius CommissionGoogle Scholar
  13. Cohen LA, Zhao Z, Pittman B, Scimeca JA (2000) Effect of intact and isoflavone-depleted soy protein on NMU-induced rat mammary tumorigenesis. Carcinogenesis 21(5):929–935CrossRefGoogle Scholar
  14. Cruz N, Capellas M, Hernandez M, Trujillo AJ, Guamis B, Ferragut V (2007) Ultra high pressure homogenization of soymilk: microbiological, physicochemical and microstructural characteristics. Food Res Int 40:725–732CrossRefGoogle Scholar
  15. Das A, Chaudhuri UR, Chakraborty R (2012) Cereal based functional food of Indian subcontinent: a review. J Food Sci Technol 49(6):665–672CrossRefGoogle Scholar
  16. Davies NT, Reid H (1979) An evaluation of the phytate, zinc, copper, iron and manganese contents of, and Zn availability from, soya-based textured-vegetable-protein meat-substitutes or meat-extenders. Br J Nutr 41:579–589CrossRefGoogle Scholar
  17. Davies CS, Nielsen SS, Nielsen NC (1987) Flavor improvement of soybean preparations by genetic removal of lipoxygenase-2. J Am Oil Chem Soc 64(10):1428–1433CrossRefGoogle Scholar
  18. Deshpande RP, Chinnan MS, McWatters KH (2008) Optimization of a chocolate-flavored, peanut–soy beverage using response surface methodology (RSM) as applied to consumer acceptability data. LWT Food Sci Technol 41:1485–1492CrossRefGoogle Scholar
  19. Deswal A, Deora NS, Mishra HN (2014) Optimization of enzymatic production process of oat milk using response surface methodology. Food Bioprocess Technol 7(2):610–618CrossRefGoogle Scholar
  20. Dhakal S, Liu C, Zhang Y, Roux KH, Sathe SK, Shridhar K, Balasubramaniam VM (2014) Effect of high pressure processing on the immunoreactivity of almond milk. Food Res Int 62:215–222CrossRefGoogle Scholar
  21. Diarra K, Zhang GN, Chen J (2005) Peanut milk and peanut milk based products production: a review. Crit Rev Food Sci Nutr 45(5):405–423CrossRefGoogle Scholar
  22. Faccin GL, Miotto LA, Vieira LN, Barreto PLM, Amante ER (2009) Chemical, sensorial and rheological properties of a new organic rice bran beverage. Rice Sci 16(3):226–234CrossRefGoogle Scholar
  23. Fallourd MJ, Viscione L (2009) Ingredient selection for stabilisation and texture optimisation of functional beverages and the inclusion of dietary fibre. In: Paquin Paul (ed) Functional and speciality beverage technology. Woodhead Publishing Limited, Cambridge, pp 19–22Google Scholar
  24. Farkas DE, Goldblith SA (1962) Studies on the kinetics of lipoxygenase inactivation using thermal and ionizing energy. J Food Sci 27:262–276CrossRefGoogle Scholar
  25. Fennema OR (1996) Food chemistry, 3rd edn. Marcel Dekker, New York (Chapter 4) Google Scholar
  26. Friedman M, Brandon DL (2001) Nutritional and health benefits of soy proteins. J Agric Food Chem 49(3):1069–1086CrossRefGoogle Scholar
  27. Frolic W, Nyman M (1988) Minerals, phytate and dietary fibre in different fractions of oat grain. J Cereal Sci 7:73–82CrossRefGoogle Scholar
  28. Fujimaki M, Arai S, Kirigaya N, Sakurai Y (1965) Studies on flavor components in soybean. Part 1. Aliphatic carbonyl compounds. Agric Biol Chem 29:855Google Scholar
  29. Fukui K, Tachibana N, Wanezaki S (2002) Isoflavone free soy protein prepared by column chromatography reduces plasma cholesterol in rats. J Agric Food Chem 50(20):5717–5721CrossRefGoogle Scholar
  30. Galvez FC, Resurreccion AV, Koehler P (1990) Optimization of processing of peanut beverage. J Sens Stud 5:1–17CrossRefGoogle Scholar
  31. Gupta RP, Gupta RR (1988) Equipment for making no-beany flavor soymilk. US4744524. 17.05.1988Google Scholar
  32. Accessed 20 Mar 2015
  33. Ikya JK, Gernah DI, Ojobo HE, Oni OK (2007) Effect of cooking temperature on some quality characteristics of soy milk. Adv J Food Sci Technol 5(5):543–546Google Scholar
  34. Iswarin SJ, Permadi B (2012) Coconut milk’s fat breaking by means of ultrasound. Int J Basic Appl Sci 12(1):1–5Google Scholar
  35. Jafari SM, He Y, Bhandari B (2007) Production of sub-micron emulsions by ultrasound and microfluidization techniques. J Food Eng 82:478–488CrossRefGoogle Scholar
  36. Johnson GW, Linda Y (1968) Simulated milk comprising soy bean flour, sesame seed flour and coco nut meal. US33868333. 04.06.1968Google Scholar
  37. Kapadia GJ, Azuine MA, Tokuda H, Takasaki M, Mukainaka T, Konoshima T, Nishino H (2002) Chemopreventive effect of resveratrol, sesamol, sesame oil and sunflower oil in the epstein-barr virus early antigen activation assay and the mouse skin two-stage carcinogenesis. Pharmacol Res 45:499–505CrossRefGoogle Scholar
  38. Kon S, Wagner JR, Guadagni DG, Horvat RJ (1970) pH adjustment control of oxidative off-flavors during grinding of raw legume seeds. J Food Sci 35(4):343–345CrossRefGoogle Scholar
  39. Kuntz DA, Nelson AI, Steinberg MP, Wei LS (1978) Control of chalkiness in soymilk. J Food Sci 43:1279–1283CrossRefGoogle Scholar
  40. Kwok KC, Niranjan K (1995) Review: effect of thermal processing on soy milk. Int J Food Sci Technol 30:263–295CrossRefGoogle Scholar
  41. Kwok KC, Qin WH, Bang JC (1993) Heat inactivation of trypsin inhibitors in soymilk at ultra-high temperatures. J Food Sci 58(4):859–862CrossRefGoogle Scholar
  42. Lee C, Beuchat LR (1992) Chemical, physical and sensory characteristics of peanut milk as affected by processing conditions. J Food Sci 57(2):401–405CrossRefGoogle Scholar
  43. Lei MG, Bassette R, Reeck GR (1981) Effect of cysteine on heat inactivation of soybean trypsin inhibitors. J Agric Food Chem 29(5):1196–1199CrossRefGoogle Scholar
  44. Lennart L, Inger A, Rickard O, Ingegerd S (1997) Homogeneous and stable cereal suspension and a method of making the same. US5686123. 11.11.1997Google Scholar
  45. Leu K (1974) Analysis of volatile compounds produced in linoleic acid oxidation catalyzed by lipoxygenase from peas, soybeans and corn germs. LWT Food Sci Technol 7:98–100Google Scholar
  46. Liener IR (1972) Nutritional value of food protein, products. In: Smith AK, Circle SJ (eds) Soybeans: chemistry and technology, vol. I: proteins. AVI Publishing, WestportGoogle Scholar
  47. Liu K (2004) Soybeans as functional foods and ingredients. AOCS Press, Champaign, Illinois, USACrossRefGoogle Scholar
  48. Lolas GM, Palamidis N, Markakis P (1976) The phytic acid, total phosphorus relationship in barley, oats, soybeans and wheat. Cereal Chem 53:867–870Google Scholar
  49. Lomer MCE, Parkes GC, Sanderson JD (2008) Review article: lactose intolerance in clinical practice—myths and realities. Aliment Pharmacol Ther 27(2):93–103CrossRefGoogle Scholar
  50. Maestri DM, Labuckas DO, Guzman CA (2000) Chemical and physical characteristics of a soybean beverage with improved flavor by addition of ethylenediaminetetraacetic acid. Grasas Aceites 51(5):316–319CrossRefGoogle Scholar
  51. Mandalari G, Nueno-Palop C, Bisignano G, Wickham MSJ, Narbad A (2008) Potential prebiotic properties of almond (Amygdalus communis L.) seeds. Appl Environ Microbiol 74(14):4264–4270CrossRefGoogle Scholar
  52. Markets and Markets (2013) Dairy alternative (beverage) market by type (Soy, almond, rice), formulation (plain, flavored, sweetened, unsweetened), channel (supermarket, health store, pharmacy, convenience store) & geography—global trends & forecast to 2018.
  53. Mitchell CR, Mitchell PR, Nissenbaum R (1988) Nutritional rice milk production. US4744992. 17.05.1988Google Scholar
  54. Murugkar DA (2014) Effect of sprouting of soybean on the chemical composition and quality of soymilk and tofu. J Food Sci Technol 51(5):915–921CrossRefGoogle Scholar
  55. Namiki M (2007) Nutraceutical functions of sesame: a review. Crit Rev Food Sci Nutr 47(7):651–673CrossRefGoogle Scholar
  56. Nelson AI, Wei LS, Steinberg MP (1971) Food products from whole soybeans. Soybean Digest 31(3):32–34Google Scholar
  57. Nelson AI, Steinberg MP, Wei LS (1976) Illinois process for preparation of soymilk. J Food Sci 41:57–61CrossRefGoogle Scholar
  58. Niki E, Yamamoto Y, Takahashi M, Komuro E, Miyama Y (1989) Inhibition of oxidation of biomembranes by tocopherol. Ann N Y Acad Sci 570:23–31CrossRefGoogle Scholar
  59. Nnam NM (1997) Chemical and sensory evaluation of vegetable milks from African yam bean Sphenostylis stenocarpa (Hochst ex A Rich) Harms and maize (Zea mays L.). Plant Foods Hum Nutr 51(3):265–275CrossRefGoogle Scholar
  60. Omoni AO, Aluko RE (2005) Soybean foods and their benefits: potential mechanisms of action. Nutr Rev 63(8):272–283CrossRefGoogle Scholar
  61. Onning G, Akesson B, Oste R, Lundquist I (1998) Effects of consumption of oat milk, soya milk, or cow’s milk on plasma lipids and antioxidative capacity in healthy subjects. Ann Nutr Metab 42:211–220CrossRefGoogle Scholar
  62. Onning G, Wallmark A, Persson M, Akesson B, Elmstahl S, Oste R (1999) Consumption of oat milk for 5 weeks lowers serum cholesterol and LDL cholesterol in free-living men with moderate hypercholesterolemia. Ann Nutr Metab 43:301–309CrossRefGoogle Scholar
  63. Prakash V, Narasinga Rao MS (1986) Physicochemical properties of oilseed protein. CRC Crit Rev Biochem Mol 20(3):265–363CrossRefGoogle Scholar
  64. Quasem JM, Mazahreh AS, Abu-Alruz K (2009) Development of vegetable based milk from decorticated sesame (Sesamum Indicum). Am J Appl Sci 6(5):888–896CrossRefGoogle Scholar
  65. Rahmati K, Tehrani MM, Daneshvar K (2014) Soy milk as an emulsifier in mayonnaise: physico-chemical, stability and sensory evaluation. J Food Sci Technol 51(11):3341–3347CrossRefGoogle Scholar
  66. Rasane P, Jha A, Sabhiki L, Kumar A, Unnikrishnan VS (2015) Nutritional advantages of oats and opportunities for its processing as value added foods: a review. J Food Sci Technol 52(2):662–675CrossRefGoogle Scholar
  67. Richardson DP (1983) Iron fortification of foods and drinks. Chem Ind 13:498–501Google Scholar
  68. Sathe SK, Wolf WJ, Roux KH, Teuber SS, Venkatachalam M, Sze-Tao KWC (2002) Biochemical characterization of amandin, the major storage protein in almond (Prunus dulcis L.). J Agric Food Chem 50(15):4333–4341CrossRefGoogle Scholar
  69. Seow CC, Gwee CN (1997) Coconut milk: chemistry and technology. Int J Food Sci Technol 32:189–201CrossRefGoogle Scholar
  70. Settaluri VS, Kandala CVK, Puppala N, Sundaram J (2012) Peanuts and their nutritional aspects—a review. Food Nutr Sci 3:1644–1650CrossRefGoogle Scholar
  71. Singh JS (2013) Effect of continuous flow high pressure throttling (CFHPT) on quality attributes of soymilk and changes during storage. A thesis submitted to the Graduate Faculty of The University of Georgia in Partial Fulfillment of the Requirements for the Degree Master of ScienceGoogle Scholar
  72. Singh T, Bains GS (1988) Grain extract-milk beverage: processing and physicochemical characteristics. J Food Sci 53(5):1387CrossRefGoogle Scholar
  73. Sizer CE (1989) Aseptic packaging of soymilk. Ch. 6. In: Lucas EW, Erickson DR, Nip WK (eds) Food uses of whole oil and protein seeds. American Oil Chemists’ Society, Champaign, pp 98–101Google Scholar
  74. Sosulski FW, Chakrabotry P, Humbert ES (1978) Legume-based imitation and blended milk products. Can Inst Food Sci Technol J 11(3):117–123CrossRefGoogle Scholar
  75. Tester RF, Karkalas J (1996) Swelling and gelatinization of oat starches. Cereal Chem 73(2):271–277Google Scholar
  76. Toma RB, Tabekhia MM (1979) Phytate and oxalate contents in sesame seed. Nutr Rep Int 20:25–31Google Scholar
  77. Truswell AS (2002) Cereal grains and coronary heart disease. Eur J Clin Nutr 56(1):1–14CrossRefGoogle Scholar
  78. Ukwuru MU, Ogbodo AC (2011) Effect of processing treatment on the quality of tigernut milk. Pak J Nutr 10(1):95–100CrossRefGoogle Scholar
  79. Valencia-Flores DC, Hernández-Herrero M, Guamis B, Ferragut V (2013) Comparing the effects of ultra-high-pressure homogenization and conventional thermal treatments on the microbiological, physical, and chemical quality of almond beverages. J Food Sci 78(2):E199–E205CrossRefGoogle Scholar
  80. Valizadeh R, Kargarsana H, Shojaei M, Mehbodnia M (2009) Effect of high intensity pulsed electric fields on microbial inactivation of cow milk. J Anim Vet Adv 8(12):2638–2643Google Scholar
  81. Welch RW (1995) Oats in human nutrition and health. In: Welch RW (ed) The oat crop. Production and utilization. Chapman and Hall, London, pp 433–479CrossRefGoogle Scholar
  82. Wien M, Oda K, Sabaté J (2014) A randomized controlled trial to evaluate the effect of incorporating peanuts into an American Diabetes Association meal plan on the nutrient profile of the total diet and cardiometabolic parameters of adults with type 2 diabetes. Nutr J 13:10CrossRefGoogle Scholar
  83. Wilkens WF, Mattick LR, Hand DB (1967) Effect of processing method on oxidative off-flavors of soybean milk. Food Technol 21:1630Google Scholar
  84. Xiang BY (2008) Effects of pulsed electric fields on structural modification and rheological properties for selected food proteins. A Thesis is submitted to Graduate Studies and Research Office in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Department of Bioresource Engineering Macdonald Campus, McGill University Sainte-Anne-de-Bellevue, Québec, CanadaGoogle Scholar
  85. Tan BH (1958) Technology of soymilk and some derivatives. Thesis. Agricultural University of WageningenGoogle Scholar
  86. Yuan S, Chang SKC, Liu Z, Xu B (2008) Elimination of trypsin inhibitor activity and beany flavor in soy milk by consecutive blanching and ultrahigh-temperature (UHT) processing. J Agric Food Chem 56(17):7957–7963CrossRefGoogle Scholar
  87. Zahra AK, Varidi M, Varidi MJ, Pourazarang H (2014) Influence of processing conditions on the physicochemical and sensory properties of sesame milk: a novel nutritional beverage. LWT Food Sci Technol 57(1):299–305CrossRefGoogle Scholar
  88. Zhang H, Li L, Tatsumi E, Isobe S (2005) High-pressure effects on proteins in soy milk. LWT Food Sci Technol 38:7–14CrossRefGoogle Scholar
  89. Zhang H, Onning G, Triantafyllou AO, Oste R (2007) Nutritional properties of oat-based beverages as affected by processing and storage. J Sci Food Agr 87(12):2294–2301CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2016

Authors and Affiliations

  1. 1.Food Grains and Oilseeds Processing DivisionICAR-Central Institute of Post-Harvest Engineering and TechnologyLudhianaIndia
  2. 2.Agricultural Structures and Environmental Control DivisionICAR-Central Institute of Post-Harvest Engineering and TechnologyLudhianaIndia

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