A powerful approach to explore the potential of medicinal plants as a natural source of odor and antioxidant compounds
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In this study an efficient and reliable method based on dynamic headspace solid-phase microextraction (HS-SPME) followed by gas chromatography–mass spectrometry (GC–qMS), was developed to establish the volatile metabolomic pattern of Thymus vulgaris L., Rosmarinus officinalis L. and Ruta chalepensis L. medicinal plants. The HS-SPME influencing parameters were investigated and the results indicated that the best extraction capability, was obtained using DVB/CAR/PDMS coating fiber at 40 °C for 45 min. Under optimal conditions, a total of 99 volatile metabolites were identified, including 53 terpenoids, 19 carbonyl compounds, 7 esters, 6 alcohols, among others. The main volatile metabolites identified in T. vulgaris include thymol (67 %), 3-octanone (9 %) and 1-octen-3-ol (7 %), while in R. officinalis the most dominant volatiles were eucalyptol (40 %), 2-decanone (20 %) and bornyl acetate (10 %). 2-Undecanone (53 %), (E)-2-octenal (28 %) and 2-nonanone (10 %) were the most relevant volatile metabolites identified in R. chalepensis. The results suggested that the HS-SPME/GC-qMS methodology is a powerful approach to establish the volatile metabolomic fingerprint of medicinal plants and providing a reliable tool for the complete characterization of these biologically active medicinal plants.
KeywordsMedicinal plants Volatile metabolite Solid-phase microextraction GC-qMS Antioxidant capacity
The support of Fundação para a Ciência e a Tecnologia (FCT) is acknowledged through the CQM pluriannual base funding/Strategic Plan: PEst-OE/QUI/UI0674/2014 and MS Portuguese Networks RNEM (REDE/1508/REM/2011).
- Barnes J, Anderson LA, Phillipson JD (2007) Rosemary (Vol, 3rd edn. In: Herbal Medicines. Pharmaceutical Press, LondonGoogle Scholar
- Cvetković ŽS, Nikolić VD, Savić IM, Savić-Gajić IM, Nikolić LB (2015) Development and validation of an RP-HPLC method for quantification of trans-resveratrol in the plant extracts. Hemijska industrija4-4.Google Scholar
- Grigore A, Paraschiv I, Colceru-Mihul S, Bubueanu C, Draghici E, Ichim M (2010) Chemical composition and antioxidant activity of Thymus vulgaris L. volatile oil obtained by two different methods. Rom Biotech Lett 15:5436–5443Google Scholar
- Halvorsen BL, Holte K, Myhrstad MCW, Barikmo I, Hvattum E, Remberg SF, Wold A-B, Haffner K, Baugerød H, Andersen LF, Moskaug Ø, Jacobs DR, Blomhoff R (2002) A systematic screening of total antioxidants in dietary plants. J Nutr 132:461–471Google Scholar
- Lin C, Yu C, Wu S, Yih K (2009) DPPH free-radical scavenging activity, total phenolic contents and chemical composition analysis of forty-two kinds of essential oils. J Food Drug Anal 17:386–395Google Scholar
- Perestrelo R, Barros AS, Rocha SM, Câmara JS (2011) Optimisation of solid-phase microextraction combined with gas chromatography-mass spectrometry based methodology to establish the global volatile signature in pulp and skin of Vitis vinifera L. grape varieties. Talanta 85:1483–1493CrossRefGoogle Scholar
- Sourmaghi MHS, Kiaee G, Golfakhrabadi F, Jamalifar H, Khanavi M (2014) Comparison of essential oil composition and antimicrobial activity of Coriandrum sativum L. extracted by hydrodistillation and microwave-assisted hydrodistillation. J Food Sci Technol 1: 1–6.Google Scholar
- VAN DEN Dool H, Dec. Kratz P (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J Chromatogr A 11: 463–471.Google Scholar