Journal of Food Science and Technology

, Volume 52, Issue 11, pp 7272–7280 | Cite as

Development of low caloric prebiotic fruit juices by dexransucrase acceptor reaction

  • Claudia P. M. L. Fontes
  • Jonas L. A. da Silva
  • Maria Cristiane Rabelo
  • Sueli Rodrigues
Original Article


In the present work pineapple, cantaloupe melon and orange juice were evaluated as substrate for oligosaccharide synthesis by dextransucrase acceptor reaction. Dextransucrase is a bacterial enzyme industrially applied for dextran synthesis using sucrose as carbon source. The acceptor reaction takes place only when a sufficient amount of another carbohydrate (acceptor) is also present in the reaction medium. The reducing sugars naturally present in fruit juices (fructose and glucose) were the acceptors for the oligosaccharides synthesis. High conversion was found for the evaluated juices and the residual sugars after the synthesis was very low (<1 %) due to the sugar consumption for oligosaccharide formation. The oligosaccharides obtained were evaluated regarding their resistance to the stomach acidity, digestive enzymes and stimulation of bifidobacterium growth. The results showed that the evaluated juices were suitable to produce ready to drink low caloric prebiotic juices by modulating the initial sugar concentration.


Dextransucrase Oligosaccharides Pineapple juice Cantaloupe melon juice Orange juice 



Authors thank to CAPES and CNPq for the scholarship and to National Institute of Tropical Fruits funded by CNPq for the financial support.


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Copyright information

© Association of Food Scientists & Technologists (India) 2015

Authors and Affiliations

  • Claudia P. M. L. Fontes
    • 1
  • Jonas L. A. da Silva
    • 1
  • Maria Cristiane Rabelo
    • 1
  • Sueli Rodrigues
    • 1
  1. 1.Food Technology Department, Biotechnology LaboratoryFederal State University of CearaFortalezaBrazil

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