Journal of Food Science and Technology

, Volume 52, Issue 9, pp 6067–6072 | Cite as

Improved functional properties of glycosylated soy protein isolate using D-glucose and xanthan gum

  • Ruiqi Li
  • Navam HettiarachchyEmail author
  • Srinivas Rayaprolu
  • Mike Davis
  • Satchithanandam Eswaranandam
  • Alok Jha
  • Pengyin Chen
Original Article


Functional properties of the soy protein need to improve to have better applications in food industry. Alkali extracted and acid precipitated soy protein isolate (SPI) was glycosylated using D-glucose (G) and Xanthan gum (X) via Maillard reaction to improve solubility. The effects of SPI to G and SPI to X ratios (SPI:G = 2:1, 1:1, and 1:2; SPI:X = 100:1 and 10:1) and incubation time (0, 6, 12, and 24 h) on the solubility and functional properties of glycosylated SPI were evaluated. The SPI:G ratio of 1:2 yielded a maximum degree of glycosylation of 71.1 %. The solubility of SPI after glycosylation significantly increased (P < 0.05) at pH 4.0–8.0 compared to SPI alone. Although the emulsion stability of glycosylated SPIs has not significantly increased (P > 0.05), the emulsifying activity improved significantly (P < 0.05). Glycosylation with SPI-X at a ratio of 10: 1 showed maximum emulsifying activity of 191.6 m2/g (SPI alone: 66.3 m2/g). Moreover, the SPI:X (ratio of 100:1) showed the maximum foaming activity (205 mL) compared to SPI alone (155 mL). The foaming stability of SPI (2.6 %) increased to 5.5 and 8.2 % when using xanthan gum at the ratio of 100:1 and 10:1, respectively. Glycosylated SPI with enhanced emulsifying and foaming properties has potential to improve the functional quality of the food products.


Soybean protein isolate Glycosylation D-glucose Xanthan gum Functional properties 


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Copyright information

© Association of Food Scientists & Technologists (India) 2015

Authors and Affiliations

  • Ruiqi Li
    • 1
  • Navam Hettiarachchy
    • 1
    Email author
  • Srinivas Rayaprolu
    • 1
  • Mike Davis
    • 1
  • Satchithanandam Eswaranandam
    • 1
  • Alok Jha
    • 2
  • Pengyin Chen
    • 3
  1. 1.Department of Food ScienceUniversity of ArkansasFayettevilleUSA
  2. 2.Center for Food Science and TechnologyBanaras Hindu UniversityVaranasiIndia
  3. 3.Department of Crop, Soil, & Environmental SciencesUniversity of ArkansasFayettevilleUSA

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