Journal of Food Science and Technology

, Volume 52, Issue 7, pp 4102–4112 | Cite as

Physicochemical properties of modified citrus pectins extracted from orange pomace

  • Simoni Spohr Venzon
  • Maria Helene Giovanetti Canteri
  • Daniel Granato
  • Bogdan Demczuk Junior
  • Giselle Maria Maciel
  • Ana Paula Stafussa
  • Charles Windson Isidoro Haminiuk
Original Article


Modified pectin is a polysaccharide rich in galacturonic acid altered by pH adjustment and thermal treatment used especially as an anti-cancer agent. The aim of this work was to study the physical and chemical properties of modified pectins extracted from orange pomace with citric and nitric acids. The galacturonic acid content, degree of esterification, Fourier Transform Infrared Spectroscopy profile, molecular weight, intrinsic viscosity, rheological properties and antioxidant activity of the pectins were evaluated. The modification process caused the de-esterification of pectins and a decrease of molecular weight due to removal of neutral sugars, maintaining the linear chain of galacturonic acid. Such changes also caused a significant increase in the in vitro antioxidant activity (p ≤ 0.05) and influenced the rheological properties of pectin, reducing its viscosity. This work showed that the modification of pectin from orange pomace with citric and nitric acids altered its structural and physical characteristics as well as its biological activity toward a free-radical.


Pectin Modified pectin Degree of esterification Rheological properties Antioxidant activity Fourier transform infrared spectroscopy 


  1. Agoda-Tandjawa G, Durand S, Gaillard C, Garnier C, Doublier JL (2012) Rheological behaviour and microstructure of microfibrillated cellulose suspensions/low-methoxyl pectin mixed systems. Effect of calcium ions. Carbohyd Polym 87:1045–1057CrossRefGoogle Scholar
  2. Arslan N (1995) Extraction of pectin from sugar-beet pulp and intrinsic viscosity–molecular weight relationship of pectin solutions. J Food Sci Tech 32:81–385Google Scholar
  3. Bélafi-Bakó K, Cserjési P, Beszédes S, Csanádi Z, Hodúr C (2012) Berry pectins: microwave-assisted extraction and rheological properties. Food Bioprocess Tech 5:1100–1105CrossRefGoogle Scholar
  4. Blumenkrantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54:484–489CrossRefGoogle Scholar
  5. Bochek AM, Zabivalova NM, Petropavlovskii GA (2001) Determination of the esterification degree of polygalacturonic acid. Russ J Appl Chem 74:796–799CrossRefGoogle Scholar
  6. Canteri MHG, Scheer AP, Wosiacki G, Ginies C, Reich M, Renard CMCG (2012) Rheological and macromolecular quality of pectin extracted with nitric acid from passion fruit rind. J Food Process Eng 35:800–809CrossRefGoogle Scholar
  7. Canteri-Schemin MH, Fertonani HCR, Waszczynskyj N, Wosiacki G (2005) Extraction of pectin from apple pomace. Braz Arch Biol Techn 48:259–266CrossRefGoogle Scholar
  8. Cardoso SM, Coimbra MA, Lopes da Silva JA (2003) Temperature dependence of the formation and melting of pectin–Ca2+ networks: a rheological study. Food Hydrocoll 17:801–807CrossRefGoogle Scholar
  9. Constenla DT, Lozano JE, Crapiste GH (1989) Thermophysical properties of clarified apple juice as a function of concentration and temperature. J Food Sci 54:663–668CrossRefGoogle Scholar
  10. Courts FL (2013) Profiling of modified citrus pectin oligosaccharide transport across Caco-2 cell monolayers. Pharma Nutrition 1:22–31CrossRefGoogle Scholar
  11. Cui SW (2005) Food carbohydrates: chemistry, physical properties, and applications. Taylor & Francis, Boca RatonCrossRefGoogle Scholar
  12. Evageliou V, Richardson RK, Morris ER (2000) Effect of pH, sugar type and thermal annealing on high-methoxy pectin gels. Carbohydr Polym 42:245–259CrossRefGoogle Scholar
  13. Fajardo AR, Lopes LC, Pereira AGB, Rubira AF, Muniz EC (2012) Polyelectrolyte complexes based on pectin–NH2 and chondroitin sulfate. Carbohydr Polym 87:1950–1955CrossRefGoogle Scholar
  14. Fissore EN, Matkovic L, Wider E, Rojas AM, Gerschenson LN (2009) Rheological properties of pectin-enriched products isolated from butternut (Cucurbita moschata Duch ex Poiret). LWT - Food Sci Technol 42:1413–1421CrossRefGoogle Scholar
  15. Fissore EN, Rojas AM, Gerschenson LN (2012) Rheological performance of pectin-enriched products isolated from red beet (Beta vulgaris L. var. conditiva) through alkaline and enzymatic treatments. Food Hydrocoll 26:249–260CrossRefGoogle Scholar
  16. Garnier C, Axelos MAV, Thibault J-F (1993) Phase diagrams of pectin-calcium systems: influence of pH, ionic strength, and temperature on the gelation of pectins with different degrees of methylation. Carbohydr Res 240:219–232CrossRefGoogle Scholar
  17. Glinsky VV, Raz A (2009) Modified citrus pectin anti-metastatic properties: one bullet, multiple targets. Carbohydr Res 344:1788–1791CrossRefGoogle Scholar
  18. Guo X, Han D, Xi H, Rao L, Liao X, Hu X, Wu J (2012) Extraction of pectin from navel orange peel assisted by ultra-high pressure, microwave or traditional heating: a comparison. Carbohydr Polym 88:441–448CrossRefGoogle Scholar
  19. Haminiuk CWI, Sierakowski MR, Vidal JRMB, Masson ML (2006) Influence of temperature on the rheological behavior of whole araca pulp (Psidium cattleianum sabine). LWT - Food Sci Technol 39:427–431CrossRefGoogle Scholar
  20. Haminiuk CWI, Maciel GM, Plata-Oviedo MSV, Peralta RM (2012) Phenolic compounds in fruits – an overview. Int J of Food Sci & Technol 47:2023–2044CrossRefGoogle Scholar
  21. Jiang Y, Du Y, Zhu X, Xiong H, Woo MW, Hu J (2012) Physicochemical and comparative properties of pectins extracted from Akebia trifoliata var. australis peel. Carbohydr Polym 87:1663–1669CrossRefGoogle Scholar
  22. Jun Yan AE, Katz A (2010) PectaSol-C modified citrus pectin induces apoptosis and inhibition of proliferation in human and mouse androgen-dependent and- independent prostate cancer cells. Integr Cancer Ther 9:197–203CrossRefGoogle Scholar
  23. Koubala BB, Kansci G, Mbome LI, Crépeau MJ, Thibault JF, Ralet MC (2008) Effect of extraction conditions on some physicochemical characteristics of pectins from “Améliorée” and “Mango” mango peels. Food Hydrocoll 22:1345–1351CrossRefGoogle Scholar
  24. Kulkarni SG, Vijayanand P (2010) Effect of extraction conditions on the quality characteristics of pectin from passion fruit peel (Passiflora edulis f. flavicarpa L.). LWT - Food Sci Technol 43:1026–1031CrossRefGoogle Scholar
  25. Liang R, Chen J, Liu W, Liu Cm YW, Yuan M, Xq Z (2012) Extraction, characterization and spontaneous gel-forming property of pectin from creeping fig (Ficus pumila Linn.) seeds. Carbohydr Polym 87:76–83CrossRefGoogle Scholar
  26. Liu L, Cao J, Huang J, Cai Y, Yao J (2010) Extraction of pectins with different degrees of esterification from mulberry branch bark. Bioresource Technol 101:3268–3273CrossRefGoogle Scholar
  27. Luzio Ga CRG (2013) Determination of degree of methylation of food pectins by chromatography. J Sci food Agri 93:2463–2469CrossRefGoogle Scholar
  28. Masuelli MA (2011) Viscometric study of pectin. Effect of temperature on the hydrodynamic properties. Int J Biol Macromol 48:286–291CrossRefGoogle Scholar
  29. Maxwell EG, Belshaw NJ, Waldron KW, Morris VJ (2012) Pectin – an emerging new bioactive food polysaccharide. Trends Food Sci Tech 24:64–73CrossRefGoogle Scholar
  30. Mensor LL, Menezes FS, Leitão GG, Reis AS, dos Santos TC, Coube CS, Leitão SG (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 15:127–130CrossRefGoogle Scholar
  31. Min B, Lim J, Ko S, Lee K-G, Lee SH, Lee S (2011) Environmentally friendly preparation of pectins from agricultural byproducts and their structural/rheological characterization. Bioresource Technol 102:3855–3860CrossRefGoogle Scholar
  32. Nangia-Makker P, Hogan V, Honjo Y, Baccarini S, Tait L, Bresalier R, Raz A (2002) Inhibition of human cancer cell growth and metastasis in nude mice by oral intake of modified citrus pectin. J Natl Cancer I 94:1854–1862CrossRefGoogle Scholar
  33. Nesic AR, Trifunovic SS, Grujic AS, Velickovic SJ, Antonovic DG (2011) Complexation of amidated pectin with poly(itaconic acid) as a polycarboxylic polymer model compound. Carbohydr Res 346:2463–2468CrossRefGoogle Scholar
  34. Ngouémazong DE, Kabuye G, Fraeye I, Cardinaels R, Van Loey A, Moldenaers P, Hendrickx M (2012) Effect of debranching on the rheological properties of Ca2 + −pectin gels. Food Hydrocoll 26:44–53CrossRefGoogle Scholar
  35. Ovodov YS (2009) Current views on pectin substances. Russ J of Bioor Chem 35:269–284CrossRefGoogle Scholar
  36. Platt D, inventor (2009) Modified pectin, United States PatentGoogle Scholar
  37. Rha HJ, Bae IY, Lee S, Yoo S-H, Chang P-S, Lee HG (2011) Enhancement of anti-radical activity of pectin from apple pomace by hydroxamation. Food Hydrocoll 25:545–548CrossRefGoogle Scholar
  38. Sengkhamparn N, Sagis LMC, de Vries R, Schols HA, Sajjaanantakul T, Voragen AGJ (2010) Physicochemical properties of pectins from okra (Abelmoschus esculentus (L.) Moench). Food Hydrocoll 24:35–41CrossRefGoogle Scholar
  39. Serrano-Cruz MR, Villanueva-Carvajal A, Morales Rosales EJ, Ramírez Dávila JF, Dominguez-Lopez A (2013) Controlled release and antioxidant activity of Roselle (Hibiscus sabdariffa L.) extract encapsulated in mixtures of carboxymethyl cellulose, whey protein, and pectin. LWT - Food Sci Technol 50:554–561CrossRefGoogle Scholar
  40. Sivam AS, Sun-Waterhouse D, Perera CO, Waterhouse GIN (2012) Exploring the interactions between blackcurrant polyphenols, pectin and wheat biopolymers in model breads; a FTIR and HPLC investigation. Food Chem 131:802–810CrossRefGoogle Scholar
  41. Steffe JF (1992) Rheological methods in food process engineering. Freeman Press, East LansingGoogle Scholar
  42. Videcoq P, Garnier C, Robert P, Bonnin E (2011) Influence of calcium on pectin methylesterase behaviour in the presence of medium methylated pectins. Carbohyd Polym 86:1657–1664CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2014

Authors and Affiliations

  • Simoni Spohr Venzon
    • 1
  • Maria Helene Giovanetti Canteri
    • 2
  • Daniel Granato
    • 3
  • Bogdan Demczuk Junior
    • 5
  • Giselle Maria Maciel
    • 4
  • Ana Paula Stafussa
    • 1
  • Charles Windson Isidoro Haminiuk
    • 1
  1. 1.Programa de Pós-Graduação em Tecnologia de Alimentos (PPGTA)Universidade Tecnológica Federal do ParanáCampus Campo MourãoBrazil
  2. 2.Coordenação de Alimentos (COEAL)Universidade Tecnológica Federal do ParanáCampus Ponta GrossaBrazil
  3. 3.Programa de Pós-Graduação em Ciência e Tecnologia de AlimentosUniversidade Estadual de Ponta GrossaPonta GrossaBrazil
  4. 4.Departamento Acadêmico de Química e Biologia (DAQBi)Universidade Tecnológica Federal do ParanáCampus Campo MourãoBrazil
  5. 5.Departamento Acadêmico de Alimentos (DALIM)Universidade Tecnológica Federal do ParanáCampus Campo MourãoBrazil

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