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Journal of Food Science and Technology

, Volume 52, Issue 6, pp 3336–3349 | Cite as

Antioxidant and sensory properties of protein hydrolysate derived from Nile tilapia (Oreochromis niloticus) by one- and two-step hydrolysis

  • Suthasinee Yarnpakdee
  • Soottawat BenjakulEmail author
  • Hordur G. Kristinsson
  • Hideki Kishimura
Original Article

Abstract

Antioxidant and sensory properties of Nile tilapia protein hydrolysates prepared by one- and two-step hydrolysis using commercial proteases were investigated. Hydrolysates prepared using single protease including Alcalase (HA), Flavourzyme (HF), Protamex (HPr) and papain (HPa) had increases in antioxidant activities as the degree of hydrolysis (DH) increased up to 40 % (P < 0.05). Amongst all hydrolysates, HA having 40 % DH showed the highest antioxidant activities. When HA was further hydrolysed by papain, the resulting hydrolysate (HAPa) exhibited the highest antioxidant activities for all assays tested (P < 0.05). ABTS radical scavenging activity and metal chelating of HAPa generally remained constant in a wide pH range (1–11) and during heating at 30–100 °C. Both activities increased in the simulated gastrointestinal tract model system, especially in intestine condition. HAPa (100–1,000 ppm) could retard lipid oxidation in β-carotene-linoleate and lecithin-liposome model systems in a dose dependent manner. Peptides in both HA and HAPa with molecular weight of 513 Da and 1,484 Da possessed the strongest ABTS radical scavenging activity and metal chelating activity, respectively. The amino acid profile of both HA and HAPa contained a high amount of hydrophobic amino acids (38.26–38.85 %) and had glutamic acid/glutamine, lysine and aspartic acid/asparagine as the dominant amino acids. However, HAPa showed a higher acceptability than did HA, owing to the lower bitterness. Therefore, the use of Alcalase in combination with papain for hydrolysis of protein isolate rendered the hydrolysate with antioxidant properties and reduced bitterness, which could serve as the functional supplement.

Keywords

Antioxidant activity Protein hydrolysate Nile tilapia Two-step hydrolysis Commercial proteases 

Notes

Acknowledgments

This research was supported by the Thailand Research Fund under the Royal Golden Jubilee Ph.D. Program to SuthasineeYarnpakdee (PHD/0226/2552) and the Grant-in-Aid for dissertation from Graduate School, Prince of Songkla University, Thailand. TRF senior research scholar program was also acknowledged for financial support.

References

  1. Benjakul S, Morrissey MT (1997) Protein hydrolysates from Pacific whiting solid wastes. J Agric Food Chem 45(9):3423–3430CrossRefGoogle Scholar
  2. Benzie IF, Strain J (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239(1):70–76CrossRefGoogle Scholar
  3. Binsan W, Benjakul S, Visessanguan W, Roytrakul S, Tanaka M, Kishimura H (2008) Antioxidative activity of Mungoong, an extract paste, from the cephalothorax of white shrimp (Litopenaeus vannamei). Food Chem 106(1):185–193CrossRefGoogle Scholar
  4. Bougatef A, Nedjar-Arroume N, Manni L, Ravallec R, Barkia A, Guillochon D, Nasri M (2010) Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinella aurita) by-products proteins. Food Chem 118(3):559–565CrossRefGoogle Scholar
  5. Buege J, Aust S (1978) Microsomal lipid peroxidation. Method Enzymol 52:302–310Google Scholar
  6. Chandrasekara A, Shahidi F (2010) Inhibitory activities of soluble and bound millet seed phenolics on free radicals and reactive oxygen species. J Agric Food Chem 59(1):428–436CrossRefGoogle Scholar
  7. Decker EA, Warner K, Richards MP, Shahidi F (2005) Measuring antioxidant effectiveness in food. J Agric Food Chem 53(10):4303–4310CrossRefGoogle Scholar
  8. FitzGerald R, O’cuinn G (2006) Enzymatic debittering of food protein hydrolysates. Biotechnol Adv 24(2):234–237CrossRefGoogle Scholar
  9. Foh MBK, Amadou I, Foh BM, Kamara MT, Xia W (2010) Functionality and antioxidant properties of tilapia (Oreochromis niloticus) as influenced by the degree of hydrolysis. Int J Mol Sci 11(4):1851–1869CrossRefGoogle Scholar
  10. Frankel EN, Huang S-W, Aeschbach R (1997) Antioxidant activity of green teas in different lipid systems. J Am Oil Chem Soc 74(10):1309–1315CrossRefGoogle Scholar
  11. Je J-Y, Lee K-H, Lee MH, Ahn C-B (2009) Antioxidant and antihypertensive protein hydrolysates produced from tuna liver by enzymatic hydrolysis. Food Res Int 42(9):1266–1272CrossRefGoogle Scholar
  12. Jun S-Y, Park P-J, Jung W-K, Kim S-K (2004) Purification and characterization of an antioxidative peptide from enzymatic hydrolysate of yellowfin sole (Limanda aspera) frame protein. Eur Food Res Technol 219(1):20–26CrossRefGoogle Scholar
  13. Khantaphant S, Benjakul S, Ghomi MR (2011a) The effects of pretreatments on antioxidative activities of protein hydrolysate from the muscle of brownstripe red snapper (Lutjanus vitta). LWT Food Sci Technol 44(4):1139–1148CrossRefGoogle Scholar
  14. Khantaphant S, Benjakul S, Kishimura H (2011b) Antioxidative and ACE inhibitory activities of protein hydrolysates from the muscle of brownstripe red snapper prepared using pyloric caeca and commercial proteases. Process Biochem 46(1):318–327CrossRefGoogle Scholar
  15. Kim S-K, Kim Y-T, Byun H-G, Nam K-S, Joo D-S, Shahidi F (2001) Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin. J Agric Food Chem 49(4):1984–1989CrossRefGoogle Scholar
  16. Klompong V, Benjakul S, Kantachote D, Hayes KD, Shahidi F (2008) Comparative study on antioxidative activity of yellow stripe trevally protein hydrolysate produced from Alcalase and Flavourzyme. Int J Food Sci Technol 43(6):1019–1026CrossRefGoogle Scholar
  17. Klompong V, Benjakul S, Yachai M, Visessanguan W, Shahidi F, Hayes K (2009) Amino acid composition and antioxidative peptides from protein hydrolysates of yellow stripe trevally (Selaroides leptolepis). J Food Sci 74(2):C126–C133CrossRefGoogle Scholar
  18. Leksrisompong P, Gerard P, Lopetcharat K, Drake M (2012) Bitter taste inhibiting agents for whey protein hydrolysate and whey protein hydrolysate beverages. J Food Sci 77(8):S282–S287CrossRefGoogle Scholar
  19. Li X, Luo Y, Shen H, You J (2012) Antioxidant activities and functional properties of grass carp (Ctenopharyngodon idellus) protein hydrolysates. J Sci Food Agric 92(2):292–298CrossRefGoogle Scholar
  20. Liaset B, Lied E, Espe M (2000) Enzymatic hydrolysis of by-products from the fish-filleting industry; chemical characterisation and nutritional evaluation. J Sci Food Agric 80(5):581–589CrossRefGoogle Scholar
  21. Meilgaard M, Civille GV, Carr BT (2007) Sensory evaluation techniques. CRC Press, Boca RatonGoogle Scholar
  22. Nalinanon S, Benjakul S, Kishimura H, Shahidi F (2011) Functionalities and antioxidant properties of protein hydrolysates from the muscle of ornate threadfin bream treated with pepsin from skipjack tuna. Food Chem 124(4):1354–1362CrossRefGoogle Scholar
  23. Ney KH (1979) Bitterness of peptides: amino acid composition and chain length. In: Boudreau JC (ed) Food Taste Chemistry. ACS Washington DC, pp 149–173Google Scholar
  24. Ovissipour M, Rasco B, Shiroodi SG, Modanlow M, Gholami S, Nemati M (2013) Antioxidant activity of protein hydrolysates from whole anchovy sprat (Clupeonella engrauliformis) prepared using endogenous enzymes and commercial proteases. J Sci Food Agric 93(7):1718–1726CrossRefGoogle Scholar
  25. Raghavan S, Kristinsson HG, Leeuwenburgh C (2008) Radical scavenging and reducing ability of tilapia (Oreochromis niloticus) protein hydrolysates. J Agric Food Chem 56(21):10359–10367CrossRefGoogle Scholar
  26. Rajapakse N, Mendis E, Byun H-G, Kim S-K (2005) Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nutr Biochem 16(9):562–569CrossRefGoogle Scholar
  27. Robinson HW, Hogden CG (1940) The biuret reaction in the deter serum proteins. J Biol Chem 135(2):727Google Scholar
  28. Saha BC, Hayashi K (2001) Debittering of protein hydrolyzates. Biotechnol Adv 19(5):355–370CrossRefGoogle Scholar
  29. Shahidi F, Han X, Synowiecki J (1995) Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chem 53(3):285–293CrossRefGoogle Scholar
  30. Steel RGD, Torrie JH (1980) Principles and procedures of statistics: a biometrical approach. McGraw-Hill, New YorkGoogle Scholar
  31. Suetsuna K, Ukeda H, Ochi H (2000) Isolation and characterization of free radical scavenging activities peptides derived from casein. J Nutr Biochem 11(3):128–131CrossRefGoogle Scholar
  32. Tavano OL (2013) Protein hydrolysis using proteases: an important tool for food biotechnology. J Mol Catal B Enzym 90:1–11CrossRefGoogle Scholar
  33. Theodore AE, Raghavan S, Kristinsson HG (2008) Antioxidative activity of protein hydrolysates prepared from alkaline-aided channel catfish protein isolates. J Agric Food Chem 56(16):7459–7466CrossRefGoogle Scholar
  34. Thiansilakul Y, Benjakul S, Shahidi F (2007a) Antioxidative activity of protein hydrolysate from round scad muscle using alcalase and flavourzyme. J Food Biochem 31(2):266–287CrossRefGoogle Scholar
  35. Thiansilakul Y, Benjakul S, Shahidi F (2007b) Compositions, functional properties and antioxidative activity of protein hydrolysates prepared from round scad (Decapterus maruadsi). Food Chem 103(4):1385–1394CrossRefGoogle Scholar
  36. Wang Y, Zhu F, Han F, Wang H (2008) Purification and characterization of antioxidative peptides from salmon protamine hydrolysate. J Food Biochem 32(5):654–671CrossRefGoogle Scholar
  37. Wróblewska B, Troszyñska A (2005) Enzymatic hydrolysis of cow’s whey milk proteins in the aspect of their utilization for the production of hypoallergenic formulas. Pol J Food Nutr Sci 14(4):349Google Scholar
  38. Wu H-C, Chen H-M, Shiau C-Y (2003) Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Res Int 36(9):949–957CrossRefGoogle Scholar
  39. Yarnpakdee S, Benjakul S, Kristinsson HG (2012) Effect of pretreatments on chemical compositions of mince from Nile tilapia (Oreochromis niloticus) and fishy odor development in protein hydrolysate. Int Aquat Res 4(1):7CrossRefGoogle Scholar
  40. Yarnpakdee S, Benjakul S, Penjamras P, Kristinsson HG (2014) Chemical compositions and muddy flavour/odour of protein hydrolysate from Nile tilapia and broadhead catfish mince and protein isolate. Food Chem 142(1):210–216CrossRefGoogle Scholar
  41. You L, Zhao M, Regenstein JM, Ren J (2010) Changes in the antioxidant activity of loach (Misgumus anguillicaudatus) protein hydrolysates during a simulated gastrointestinal digestion. Food Chem 120(3):810–816CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2014

Authors and Affiliations

  • Suthasinee Yarnpakdee
    • 1
  • Soottawat Benjakul
    • 1
    Email author
  • Hordur G. Kristinsson
    • 2
    • 3
  • Hideki Kishimura
    • 4
  1. 1.Department of Food Technology, Faculty of Agro-IndustryPrince of Songkla UniversityHat YaiThailand
  2. 2.Matis - Icelandic Food and Biotechnology R & DReykjavikIceland
  3. 3.Department of Food Science and Human NutritionUniversity of FloridaGainesvilleUSA
  4. 4.Laboratory of Marine Products and Food Science, Research Faculty of Fisheries SciencesHokkaido UniversityHakodateJapan

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