Advertisement

Journal of Food Science and Technology

, Volume 51, Issue 12, pp 3910–3917 | Cite as

In vitro antidiabetic and inhibitory potential of turmeric (Curcuma longa L) rhizome against cellular and LDL oxidation and angiotensin converting enzyme

  • P. C. Lekshmi
  • Ranjith Arimboor
  • V. M. Nisha
  • A. Nirmala MenonEmail author
  • K. G. Raghu
Original Article

Abstract

Turmeric (Curcuma longa L) rhizome extracts were evaluated for their antidiabetic, antihypertensive and antioxidant potentials. α-Glucosidase (0.4 μg/mL) and α-amylase (0.4 μg/mL) inhibitory potential of turmeric ethyl acetate extract was significantly higher than those of the reference drug acarbose (17.1 μg/mL and 290.6 μg/mL respectively). Protein glycation inhibitory potential of ethyl acetate extract was 800 times higher than that of ascorbic acid. High potential of ethyl acetate extract to scavenge free radicals and to reduce LDL oxidation and cellular oxidative stress was also revealed. The positive correlation obtained between the free radical scavenging capacity of the extracts and their antiglycation potential further confirmed the role of antioxidants in controlling glycation reactions. Ethyl acetate extract was also found as effective in reducing hypertension by inhibiting angiotensin converting enzyme (ACE). Antidiabetic, ACE inhibitory and antioxidant capacities of the extracts were in the order of their curcumin contents.

Keywords

Curcuma longa L Glucosidase Angiotensin Antiglycation LDL oxidation and antioxidant 

Notes

Acknowledgments

We gratefully acknowledge the financial support provided by Council of Scientific and Industrial Research, India.

References

  1. Annapurna A, Suhasin G, Akondi RB, Prakash GJ, Reddy CS (2011) Anti-cancer activity of curcuma longa linn. (Turmeric). J Pharm Res 4:1274–1276Google Scholar
  2. Apostolidis E, Kwon YI, Shetty K (2007) Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Inno Food Sci Emerg Tech 8:46–54CrossRefGoogle Scholar
  3. Barnett AH (1994) Diabetes and hypertension. Br Med Bull 50:397–407Google Scholar
  4. Braga MEM, Leal PF, Carvalho JE, Meireles MAA (2003) Comparison of yield, composition and antioxidant activity of turmeric (Curcuma longa L.) extracts obtained using various techniques. J Agric Food Chem 51:6604–6611CrossRefGoogle Scholar
  5. Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 28:25–30CrossRefGoogle Scholar
  6. Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee KR (2004) Turmeric and curcumin: biological actions and medicinal applications. Curr Sci 87:44–53Google Scholar
  7. Cushman DW, Cheung HS (1971) Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 20:1637–1648CrossRefGoogle Scholar
  8. Dyer DG, Dunn JA, Thorpe SR, Bailie KE, Lyons TJ, Mc Cance DR, Baynes JW (1993) Accumulation of Maillard reaction products in skin collagen in diabetes and aging. J Clin Invest 91:2463–2469CrossRefGoogle Scholar
  9. Gin H, Rigalleau V (2000) Post-prandial hyperglycemia and diabetes. Diab Metab 26:265–272Google Scholar
  10. Greismacher A, Kindhauser M, Andert SE, Anderty ME, Schreiner W, Toma C, Mueller MD (1995) Enhanced serum levels of thiobarbituric-acid-reactive substances in diabetes mellitus. Am J Med 98:469–474CrossRefGoogle Scholar
  11. Gupta AP, Gupta MM, Kumar S (1999) Simultaneous determination of curcuminoids in curcuma samples using high performance thin layer chromatography. J Liq Chrom Rel Tech 22:1561–1569CrossRefGoogle Scholar
  12. Jayaprakasha GK, Rao LJM, Sakariah KK (2005) Chemistry and biological activities of C. longa. Trend Food Sci Technol 16:533–548CrossRefGoogle Scholar
  13. Jedsadayanmata A (2005) In vitro antiglycation activity of arbutin. Naresuan Univ J 13:35–41Google Scholar
  14. Kim HY, Kim K (2003) Protein glycation inhibitory and antioxidative activities of some plant extracts in vitro. J Agri Food Chem 2003(51):1586–1591CrossRefGoogle Scholar
  15. Kotamballi N, Chidambara M, Ravendra PS, Jayaprakasha GK (2002) Antioxidant activities of grape (Vitis vinifera) pomace extracts. J Agric Food Chem 50:5909–5914CrossRefGoogle Scholar
  16. Kshirsagar AV, Joy MS, Hogan SL, Falk RJ, Colindres RE (2000) Effect of ACE inhibitors in diabetic and nondiabetic chronic renal disease: a systematic overview of randomized placebocontrolled trials. Am J Kidney Dis 35:695–707CrossRefGoogle Scholar
  17. Lekshmi PC, Arimboor R, Raghu KG, Nirmala MA (2011) Turmerin, the antioxidant protein from turmeric (Curcuma longa) exhibits antihyperglycemic effects. Nat Prod Res. doi: 10.1080/14786419.2011.589386
  18. Matsuura N, Aradate T, Sasaki C et al (2002) Screening system for the Maillard reaction inhibitor from natural products extacts. J Health Sci 48:520–526CrossRefGoogle Scholar
  19. Murugan P, Pari L (2007) Influence of tetrahydrocurcumin on erythrocyte membrane bound enzymes and antioxidant status in experimental type 2 diabetic rats. J Ethnopharmacol 113:479–486CrossRefGoogle Scholar
  20. Naito M, Wu X, Nomura H, Kodama M, Kato Y, Kato Y, Osawa T (2002) The protective effects of tetrahydrocurcumin on oxidative stress in cholesterol-fed rabbits. J Atheroscler Thromb 9:243–250CrossRefGoogle Scholar
  21. Nathan DM (1993) Long term complications of diabetes mellitus. N Engl J Med 328:1676–1685CrossRefGoogle Scholar
  22. Nishiyama T, Mae T, Kishida H et al (2005) Curcuminoids and sesquiterpenoids in turmeric (Curcuma longa L.) suppress an increase in blood glucose level in type 2 diabetic KK-Ay mice. J Agric Food Chem 53:959–963CrossRefGoogle Scholar
  23. Pathania V, Gupta AP, Singh B (2006) Improved HPTLC method for determination of curcuminoids from Curcuma longa. J Liq Chromatogr Rel Tech 29:877–887CrossRefGoogle Scholar
  24. Paya M, Halliwell B, Hoult JRS (1992) Interactions of a series of coumarins with reactive oxygen species. Scavenging of superoxide, hypochlorous acid and hydroxyl radicals. Biochem Pharmacol 44:205–214CrossRefGoogle Scholar
  25. Qui H, Zhan Q, Zhao T, Hu R, Zhan K, Li Z (2006) Invitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa (chlorophyta). Bioorg Med Chem Lett 16:2441–2445CrossRefGoogle Scholar
  26. Ramírez-Tortosa MC, Mesa MD, Aguilera MC, Quiles JL, Baro L, Ramirez-Tortosa CL, Martinez-Victoria E, Gil A (1999) Oral administration of a turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis. Atherosclerosis 147:371–378CrossRefGoogle Scholar
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med 26:1231–1237CrossRefGoogle Scholar
  28. Sato M, Ramarathnam N, Suzuki Y, Ohkuho T, Takeuchi M, Ochi H (1996) Varietal differences in the phenolic content and superoxide potential of wines from different sources. J Agric Food Chem 44:37–41CrossRefGoogle Scholar
  29. Singh R, Barden A, Mori T, Beilin L (2001) Advanced glycation end-products: a review. Diabetologia 44:129–146CrossRefGoogle Scholar
  30. Singh G, Kapoor IPS, Pratibha S, Heluani CSD, Lampasona MPD, Catalan CAN (2010) Comparative study of chemical composition and antioxidant activity of fresh and dry rhizomes of turmeric (Curcuma longa Linn). Food Chem Toxicol 48:1026–1031CrossRefGoogle Scholar
  31. Vasan S, Foiles P, Founds H (2003) Therapeutic potential of breakers of advanced glycation end product-protein cross links. Arch Biochem Biophys 419:89–96CrossRefGoogle Scholar
  32. Voziyan PA, Khalifah RG, Thibaudeau C et al (2003) Modification of proteins in vitro by physiological levels of glucose. J Biol Chem 278:46616–46624CrossRefGoogle Scholar
  33. Wichitnithad W, Jongaroonngamsang N, Pummangura S, Rojsitthisak P (2009) A simple isocratic HPLC method for the simultaneous determination of curcuminoids in commercial turmeric extracts. Phytochem Anal 20:314–319CrossRefGoogle Scholar
  34. Witztum JL, Steinberg D (1991) Role of oxidized low density lipoprotein in atherogenesis. J Clin Invest 88:1785–1792CrossRefGoogle Scholar
  35. Yamaguchi F, Ariga T, Yoshimura Y, Nakazawa H (2000) Antioxidative and antiglycation activity of garcinol from Garcinia indica fruit rind. J Agri Food Chem 48:180–185CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2013

Authors and Affiliations

  • P. C. Lekshmi
    • 1
  • Ranjith Arimboor
    • 1
  • V. M. Nisha
    • 1
  • A. Nirmala Menon
    • 1
    Email author
  • K. G. Raghu
    • 1
  1. 1.Agroprocessing and Natural Products DivisionCSIR-National Institute for Interdisciplinary Science and TechnologyThiruvananthapuramIndia

Personalised recommendations