Journal of Food Science and Technology

, Volume 50, Issue 2, pp 266–274 | Cite as

Effect of combination of dietary fish protein and fish oil on lipid metabolism in rats

  • Ryota Hosomi
  • Kenji Fukunaga
  • Hirofumi Arai
  • Seiji Kanda
  • Toshimasa Nishiyama
  • Munehiro Yoshida
Original Article


This study examined the effects of fish protein in combination with fish oil on rat lipid metabolism. Male Wistar rats were divided into four groups and fed an AIN93G-based diet with casein (20%) + soybean oil (7%), casein (10%) + fish protein (10%) + soybean oil (7%), casein (20%) + soybean oil (5%) + fish oil (2%), and casein (10%) + fish protein (10%) + soybean oil (5%) + fish oil (2%) for 4 weeks. The dietary combination of fish protein and fish oil decreased the contents of serum triacylglycerol, serum cholesterol, liver triacylglycerol and liver cholesterol in addition to altering liver lipid fatty acid composition. These effects are partly due to the increase in fecal cholesterol, bile acid excretion, and increased enzyme activities of fatty acid β-oxidation in the liver. These data suggest that combined intake of fish protein and fish oil lead to both hypocholesterolemic and hypotriglyceridemic in serum and the liver, while sole intake of fish protein or fish oil decrease only cholesterol and triglyceride levels, respectively. These results suggest that combined intake of fish protein and fish oil may play beneficial roles in the prevention of lifestyle-related diseases as compared with sole fish protein intake.


Fish protein Fish oil n-3 polyunsaturated fatty acid Lipid metabolism 



We thank Dr. Hayato Maeda of Hirosaki University, for assistance with the Real-time PCR analyses and Kenta Hayashi and Takanori Shimizu of Kansai University for their help with animal care.


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Copyright information

© Association of Food Scientists & Technologists (India) 2011

Authors and Affiliations

  • Ryota Hosomi
    • 1
  • Kenji Fukunaga
    • 1
  • Hirofumi Arai
    • 2
  • Seiji Kanda
    • 3
  • Toshimasa Nishiyama
    • 3
  • Munehiro Yoshida
    • 1
  1. 1.Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and BioengineeringKansai UniversitySuitaJapan
  2. 2.Division of Biotechnology and Environmental ChemistryKitami Institute of TechnologyKitamiJapan
  3. 3.Department of Public HealthKansai Medical UniversityMoriguchiJapan

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