Journal of Food Science and Technology

, Volume 50, Issue 2, pp 266–274 | Cite as

Effect of combination of dietary fish protein and fish oil on lipid metabolism in rats

  • Ryota Hosomi
  • Kenji Fukunaga
  • Hirofumi Arai
  • Seiji Kanda
  • Toshimasa Nishiyama
  • Munehiro Yoshida
Original Article

Abstract

This study examined the effects of fish protein in combination with fish oil on rat lipid metabolism. Male Wistar rats were divided into four groups and fed an AIN93G-based diet with casein (20%) + soybean oil (7%), casein (10%) + fish protein (10%) + soybean oil (7%), casein (20%) + soybean oil (5%) + fish oil (2%), and casein (10%) + fish protein (10%) + soybean oil (5%) + fish oil (2%) for 4 weeks. The dietary combination of fish protein and fish oil decreased the contents of serum triacylglycerol, serum cholesterol, liver triacylglycerol and liver cholesterol in addition to altering liver lipid fatty acid composition. These effects are partly due to the increase in fecal cholesterol, bile acid excretion, and increased enzyme activities of fatty acid β-oxidation in the liver. These data suggest that combined intake of fish protein and fish oil lead to both hypocholesterolemic and hypotriglyceridemic in serum and the liver, while sole intake of fish protein or fish oil decrease only cholesterol and triglyceride levels, respectively. These results suggest that combined intake of fish protein and fish oil may play beneficial roles in the prevention of lifestyle-related diseases as compared with sole fish protein intake.

Keywords

Fish protein Fish oil n-3 polyunsaturated fatty acid Lipid metabolism 

Notes

Acknowledgements

We thank Dr. Hayato Maeda of Hirosaki University, for assistance with the Real-time PCR analyses and Kenta Hayashi and Takanori Shimizu of Kansai University for their help with animal care.

References

  1. Berge RK, Madsen L, Vaagenes H, Tronstad KJ, Göttlicher M, Rustan AC (1999) In contrast with docosahexaenoic acid, eicosapentaenoic acid and hypolipidaemic derivatives decrease hepatic synthesis and secretion of triacylglycerol by decreased diacylglycerol acyltransferase activity and stimulation of fatty acid oxidation. Biochem J 343:191–197CrossRefGoogle Scholar
  2. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefGoogle Scholar
  3. Brandsch C, Shukla A, Hirche F, Stangl GI, Eder K (2006) Effect of proteins from beef, pork, and turkey meat on plasma and liver lipids of rats compared with casein and soy protein. Nutrition 22:1162–1170CrossRefGoogle Scholar
  4. Bruusgaard A, Sorensen H, Gilhuus-Moe CC, Skålhegg BA (1977) Bile acid determination with different preparations of 3α-hydroxysteroid dehydrogenase. Clin Chim Acta 77:387–395CrossRefGoogle Scholar
  5. Cromwell WC, Otvos JD (2004) Low-density lipoprotein particle number and risk for cardiovascular disease. Curr Atheroscler 6:381–387CrossRefGoogle Scholar
  6. Frøyland L, Vaagenes H, Asiedu DK, Garras A, Lie O, Berge RK (1996) Chronic administration of eicosapentaenoic acid and docosahexaenoic acid as ethyl esters reduced plasma cholesterol and changed the fatty acid composition in rat blood and organs. Lipids 31:169–178CrossRefGoogle Scholar
  7. Frøyland L, Madsen L, Vaagenes H, Totland GK, Auwerx J, Kryvi H, Staels B, Berge RK (1997) Mitochondrion is the principal target for nutritional and pharmacological control of triglyceride metabolism. J Lipid Res 38:1851–1858Google Scholar
  8. Grønn M, Christensen E, Hagve TA, Christophersen BO (1992) Effects of dietary purified eicosapentaenoic acid (20:5 (n-3)) and docosahexaenoic acid (22:6(n-3)) on fatty acid desaturation and oxidation in isolated rat liver cells. Biochim Biophys Acta 1125:35–43CrossRefGoogle Scholar
  9. Horton JD, Bashmakov Y, Shimomura I, Shimano H (1998) Regulation of sterol regulatory element binding proteins in livers of fasted and refed mice. Proc Natl Acad Sci USA 95:5987–5992CrossRefGoogle Scholar
  10. Hosomi R, Fukunaga K, Arai H, Nishiyama T, Yoshida M (2009) Effects of dietary fish protein on serum and liver lipid concentrations in rats and the expression of hepatic genes involved in lipid metabolism. J Agric Food Chem 57:9256–9262CrossRefGoogle Scholar
  11. Ide T, Watanabe M, Sugano M, Yamamoto I (1987) Activities of liver mitochondrial and peroxisomal fatty acid oxidation enzymes in rats fed trans fat. Lipids 22:6–10CrossRefGoogle Scholar
  12. Ide T, Hong DD, Ranasinghe P, Takahashi Y, Kushiro M, Sugano M (2004) Interaction of dietary fat types and sesamin on hepatic fatty acid oxidation in rats. Biochim Biophys Acta 1682:80–91CrossRefGoogle Scholar
  13. Ikeda I, Kudo M, Hamada T, Nagao K, Oshiro Y, Kato M, Sugawara T, Yamahira T, Ito H, Tamaru S, Sato M, Imaizumi K, Nagaoka S, Yanagita T (2009) Dietary soy protein isolate and its undigested high molecular fraction upregulate hepatic ATP-binding cassette transporter G5 and ATP-binding cassette transporter G8 mRNA and increase biliary secretion of cholesterol in rats. J Nutr Sci Vitaminol (Tokyo) 55:252–256CrossRefGoogle Scholar
  14. Jacobson TA, Miller M, Schaefer EJ (2007) Hypertriglyceridemia and cardiovascular risk reduction. Clin Ther 29:763–777CrossRefGoogle Scholar
  15. Katan MB, Vroomen LH, Hermus RJ (1982) Reduction of casein-induced hypercholesterolemia and atherosclerosis in rabbits and rats by dietary glycine, arginine and alanine. Atherosclerosis 43:381–391CrossRefGoogle Scholar
  16. Kelley DS, Kletzien RF (1984) Ethanol modulation of the hormonal and nutritional regulation of glucose 6-phosphate dehydrogenase activity in primary cultures of rat hepatocytes. Biochem J 217:543–549Google Scholar
  17. Kelley DS, Nelson GJ, Hunt JE (1986) Effect of prior nutritional status on the activity of lipogenic enzymes in primary monolayer cultures of rat hepatocytes. Biochem J 235:87–90Google Scholar
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  19. Madani S, Lopez S, Blond JP, Prost J, Belleville J (1998) Highly purified soybean protein is not hypocholesterolemic in rats but stimulates cholesterol synthesis and excretion and reduces polyunsaturated fatty acid biosynthesis. J Nutr 128:1084–1091Google Scholar
  20. Markwell MA, McGroarty EJ, Bieber LL, Tolbert NE (1973) The subcellular distribution of carnitine acyltransferases in mammalian liver and kidney. A new peroxisomal enzyme. J Biol Chem 248:3426–3432Google Scholar
  21. Minoura T, Takata T, Sakaguchi M, Takada H, Yamamura M, Hioki K, Yamamoto M (1988) Effect of dietary eicosapentaenoic acid on azoxymethane-induced colon carcinogenesis in rats. Cancer Res 48:4790–4794Google Scholar
  22. Osler M, Helms-Andreasen A, Heitmann B, Høidrup S, Gerdes U, Mørch-Jørgensen L, Schroll M (2002) Food intake patterns and risk of coronary heart disease: a prospective cohort study examining the use of traditional scoring techniques. Eur J Clin Nutr 56:568–574CrossRefGoogle Scholar
  23. Pan DA, Hulbert AJ, Storlien LH (1994) Dietary fats, membrane phospholipids and obesity. J Nutr 124:1555–1565Google Scholar
  24. Paton CM, Ntambi JM (2009) Biochemical and physiological function of stearoyl-CoA desaturase. Am J Physiol Endocrinol Metab 297:E28–E37CrossRefGoogle Scholar
  25. Pereira MA, O’Reilly E, Augustsson K, Fraser GE, Goldbourt U, Heitmann BL, Hallmans G, Knekt P, Liu S, Pietinen P, Spiegelman D, Stevens J, Virtamo J, Willett WC, Ascherio A (2004) Dietary fiber and risk of coronary heart disease: a pooled analysis of cohort studies. Arch Intern Med 164:370–376CrossRefGoogle Scholar
  26. Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951Google Scholar
  27. Sabeva N, Liu J, Graf GA (2009) The ABCG5 ABCG8 sterol transporter and phytosterols: implications for cardiometabolic disease. Curr Opin Endocrinol Diab Obes 16:172–177CrossRefGoogle Scholar
  28. Serougne C, Ferezou J, Rukaj A (1984) Effect of excess dietary L-cystine on the rat plasma lipoproteins. Ann Nutr Metab 28:311–320CrossRefGoogle Scholar
  29. Shukla A, Bettzieche A, Hirche F, Brandsch C, Stangl GI, Eder K (2006) Dietary fish protein alters blood lipid concentrations and hepatic genes involved in cholesterol homeostasis in the rat model. Br J Nutr 96:674–682Google Scholar
  30. Simopoulos AP (2002) Omega-3 fatty acids in inflammation and autoimmune diseases. J Am Coll Nutr 21:495–505Google Scholar
  31. Spielmann J, Noatsch A, Brandsch C, Stangl GI, Eder K (2008) Effects of various dietary arginine and lysine concentrations on plasma and liver cholesterol concentrations in rats. Ann Nutr Metab 53:223–233CrossRefGoogle Scholar
  32. Sugano M, Goto S, Yamada Y, Yoshida K, Hashimoto Y, Matsuo T, Kimoto M (1990) Cholesterol-lowering activity of various undigested fractions of soybean protein in rats. J Nutr 120:977–985Google Scholar
  33. Sugiyama K, Ohkawa S, Muramatsu K (1986) Relationship between amino acid composition of diet and plasma cholesterol level in growing rats fed a high cholesterol diet. J Nutr Sci Vitaminol (Tokyo) 32:413–423CrossRefGoogle Scholar
  34. Wergedahl H, Liaset B, Gudbrandsen OA, Lied E, Espe M, Muna Z, Mørk S, Berge RK (2004) Fish protein hydrolysate reduces plasma total cholesterol, increases the proportion of HDL cholesterol, and lowers acyl-CoA:cholesterol acyltransferase activity in liver of Zucker rats. J Nutr 134:1320–1327Google Scholar
  35. Yanagita T, Wang YM, Nagao K, Ujino Y, Inoue N (2005) Conjugated linoleic acid-induced fatty liver can be attenuated by combination with docosahexaenoic acid in C57BL/6N mice. J Agric Food Chem 53:9629–9633CrossRefGoogle Scholar
  36. Zhang X, Beynen AC (1993) Influence of dietary fish proteins on plasma and liver cholesterol concentrations in rats. Br J Nutr 69:767–777CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2011

Authors and Affiliations

  • Ryota Hosomi
    • 1
  • Kenji Fukunaga
    • 1
  • Hirofumi Arai
    • 2
  • Seiji Kanda
    • 3
  • Toshimasa Nishiyama
    • 3
  • Munehiro Yoshida
    • 1
  1. 1.Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and BioengineeringKansai UniversitySuitaJapan
  2. 2.Division of Biotechnology and Environmental ChemistryKitami Institute of TechnologyKitamiJapan
  3. 3.Department of Public HealthKansai Medical UniversityMoriguchiJapan

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