Journal of Food Science and Technology

, Volume 50, Issue 1, pp 26–34 | Cite as

Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6

  • Jayesh Jagannath Ahire
  • Narendra Uttamrao Mokashe
  • Hemant Jagatrao Patil
  • Bhushan Liladhar ChaudhariEmail author
Original Article


Folate producing Lactobacillus sp. CD6 isolated from fermented milk showed 98% similarity with Lactobacillus helveticus based on 16S rRNA gene sequence analysis. It was found to produce a folic acid derivative 5-methyl tetrahydrofolate (5-MeTHF). The intracellular cell-free extract of strain demonstrated antioxidative activity with the inhibition rate of ascorbate autoxidation in the range of 27.5% ± 3.7%. It showed highest metal ion chelation ability for Fe2+ (0.26 ± 0.06 ppm) as compared to Cu2+. The DPPH (α,α-Diphenyl-β-Picrylhydrazyl) radical scavenging activity for intact cells were found to be 24.7% ± 10.9% proved its antioxidative potential. Furthermore, it demonstrated 14.89% inhibition of epinephrine autoxidation, 20.9 ± 1.8 μg cysteine equivalent reducing activity and 20.8% ± 0.9% hydroxyl radical scavenging effect. The strain was evaluated for probiotic properties as per WHO and FAO guidelines. It showed 90.61% survival at highly acidic condition (pH 2.0), 90.66% viability in presence of synthetic gastric juice and 68% survivability at 0.5% bile concentration for 24 h. It was susceptible to many antibiotics which reduces the prospect to offer resistance determinants to other organisms if administered in the form of probiotic preparations. It showed in vitro mucus binding and antimicrobial activity against enteric pathogens like Salmonella typhimurium (NCIM 2501), Streptococcus pyogenes (NCIM 2608), and Staphylococcus aureus (NCIM 5021) and moreover it showed non- hemolytic activity on sheep blood agar.


Probiotic Lactobacillus helveticus Fermented milk 5-methyl tetrahydrofolate Ascorbate autoxidation 



The authors are thankful to University Grants Commission, New Delhi for financial support.


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Copyright information

© Association of Food Scientists & Technologists (India) 2011

Authors and Affiliations

  • Jayesh Jagannath Ahire
    • 1
  • Narendra Uttamrao Mokashe
    • 1
  • Hemant Jagatrao Patil
    • 1
    • 2
  • Bhushan Liladhar Chaudhari
    • 1
    Email author
  1. 1.Department of Microbiology, School of Life SciencesNorth Maharashtra UniversityMaharashtraIndia
  2. 2.National Bureau of Agriculturally Important MicroorganismsMauIndia

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