Evaluation of irradiation in foods using DNA Comet assay
- 135 Downloads
Comet assay is a rapid, inexpensive and sensitive biological technique to detect DNA damage in food stuffs by irradiation. In this study the Comet assay is applied on foods of plant and animal origins. Samples were irradiated by using Co-60 gamma-radiation source. The applied doses were 2, 6 and 10 kGy for food of plant origin and 0.5, 1 and 2 kGy for meat items. The un-irradiated and irradiated samples were clearly differentiated on the basis of DNA fragmentation. During the electrophoresis study, it was found that in un-irradiated cells DNA remained intact and appeared as Comets without tail whereas in irradiated cells Comets with tails were visible due to stretching of fragmented DNA. Moreover, it was also revealed that the DNA tail length was dose dependent. Dry food stuffs (seeds) showed good results as compared to moist foods (meat, fruits and vegetables) due to the absence of background damage.
KeywordsComet assay DNA fragmentation Irradiation Electrophoresis Absorbed dose
We are grateful to the authorities of Nuclear Institute for Food and Agriculture (NIFA), Peshawar, for providing the gamma irradiation facility.
- Bhatti AI, Kwon JH, Rehman SU (2009) Luminescence characteristics for the detection of radiation treatment of shellfish by using isolated minerals. J Food Sci Technol 46:279–282Google Scholar
- Cerda H, Deliancee H, Haine H, Rupp H (1997) The DNA Comet assay as a rapid screening technique to control irradiated food. Mutat Res, Fundam Mol Mech Mutagen 375:167–181Google Scholar
- Collins RA, Guo MA, Duthie SJ (1995) The kinetics of repair and oxidative DNA damage (Strand breaks and oxidized pyrimidines) in human cells. Mutat Res 336:69–77Google Scholar
- Cutrubinis M, Chirita D, Savu D, Secu CE, Secu RM, Ponta C (2007) Preliminary study on detection of irradiated food stuffs from the Romanian Market. Radiat Phys Chem 66:445–458Google Scholar
- ECS (2001) Food stuffs DNA Comet assay for the detection of irradiated food stuffs screening method. EN 13784, European Committee for Standardization, Brussels BelgiumGoogle Scholar
- Fairbairn WD, Olive PL, Neill KLO (1995) The Comet assay: a comprehensive review. Mutat Res 339:37–59Google Scholar
- Park HJ, Hyun CK, Jeong SK, Manst J, Shin HK (2000) Use of the single cell gel electrophoresis assay (Comet assay) as a technique for monitoring low-temperature treated and irradiated muscle tissue. Int J Food Sci Technol 35:555–561Google Scholar
- Wong VWC, Szeto YT, Collins AR, Benzie IFF (2005) The Comet assay: a biomonitoring tool for neutraceutical research. Curr Top Nutraceutical Res 3(1):1–14Google Scholar