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Bulletin de la Société de pathologie exotique

, Volume 105, Issue 4, pp 305–310 | Cite as

Impact retardé des moustiquaires imprégnées de lambdacyhalothrine sur la fréquence de la mutation kdr chez Anopheles gambiae s.s. (Diptera : Culicidae) au nord de la Côte-d’Ivoire

  • M. TouréEmail author
  • P. Carnevale
  • F. Chandre
Entomologie Médicale / Medical Entomology

Résumé

Des moustiquaires imprégnées de lambdacyhalothrine à raison de 15 mg m.a./m2 ont été distribuées dans quatre villages de la région de Korhogo au nord de la Côte-d’Ivoire et quatre autres villages n’ayant pas reçu de moustiquaire ont été suivis comme témoins. Les moustiques ont été capturés chaque trimestre entre juin 1999 et août 2000 dans les huit villages, puis testés pour déterminer leurs génotypes vis-à-vis de la mutation kdr L1014F et pour déterminer les formes moléculaires M et S d’Anopheles gambiae s.s. Les fréquences de l’allèle résistant au locus de la mutation kdr L1014F ont été très élevées, variant entre 0,82 et 0,96. Elles ont significativement augmenté au 14e mois chez les moustiques issus des villages traités par moustiquaires imprégnées (0,94) comparés à ceux issus des villages témoins (0,87) [p = 0,013]. La forme moléculaire S d’An. gambiae s.s. a été majoritaire (97 %). Aucun hétérozygote entre les formes M et S n’a été observé dans les villages de la région de Korhogo. Tous les individus de la formeMse sont révélés homozygotes sensibles au locus kdr.

Mots clés

Anopheles gambiae s.s. Paludisme Moustiquaires imprégnées Mutation kdr Sénoufo Korhogo Folonfokaha Fapaha Nambekaha Nongotchénékaha Ounantiékaha Binguébougou Gbahouakaha Kaforo Côte-d’Ivoire Afrique intertropicale 

Late impact of lambdacyhalothrin-treated nets on kdr allelic frequency in Anopheles gambiae s.s. (Diptera: Culicidae) from northern Côte-d’Ivoire

Abstract

Nets treated with lambdacyhalothrin 15 mg a.i./m2 were distributed in four villages of Korhogo area in northern Côte-d’Ivoire, and four other villages without nets were followed as controls. Mosquitoes were collected every three months from June 1999 to August 2000 in the villages, and then tested to determine their genotypes for kdr mutation L1014F conferring cross-resistance to pyrethroids and DDT and for M and S molecular forms of Anopheles gambiae s.s. The frequencies of the resistant allele at kdr mutation L1014F locus were very high and they varied from 0.82 to 0.96. Before the trial, the genotypic frequencies of specimens at kdr locus did not show any significant difference. However, they significantly increased in mosquitoes from treated villages (0.94) compared to those from control villages (0.87) on month 14 (P = 0.013). No significant difference was observed between mosquitoes collected outside and inside the houses (P < 0.05). In contrary, a significant difference was observed before and after the trial between mosquitoes from villages with two cycles of rice cultivation per year and those from villages without rice cultivation (P = 0.008 and P = 0.012). Nine out of twelve populations of field mosquitoes showed Hardy-Weinberg disequilibrium at kdr locus (P < 0.05). The S and M molecular forms of An. gambiae s.s. were sympatric in Korhogo area and the S form represents 97%. No hybrid betweenMand S forms was observed. All individuals of the M form were homozygote susceptible at kdr locus.

Keywords

Anopheles gambiae s.s. Malaria Treated nets kdr mutation Sénoufo Korhogo Folonfokaha Fapaha Nambekaha Nongotchénékaha Ounantiékaha Binguébougou Gbahouakaha Kaforo Côte-d’Ivoire Sub-Saharan Africa 

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Références

  1. 1.
    Alonso PL, Lindsay SW, Armstrong JRM, et al (1991) The effect of insecticide treated bed nets on mortality of Gambian children. Lancet 337(8756):1499–1502PubMedCrossRefGoogle Scholar
  2. 2.
    Awolola TS, Oyewole IO, Amajoh CN, et al (2005) Distribution of the molecular forms of Anopheles gambiae and pyrethroid knockdown resistance gene in Nigeria. Acta Trop 95(3):204–209PubMedCrossRefGoogle Scholar
  3. 3.
    Binka FN, Kubaje A, Adjuik M, et al (1996) Impact of permethrin impregnated bednets on child mortality in Kassena-Nankana district, Ghana: a randomized controlled trial. Trop Med Int Health 1(2):147–154PubMedCrossRefGoogle Scholar
  4. 4.
    Chandre F, Darriet F, Manga L, et al (1999) Status of pyrethroid resistance in Anopheles gambiae sensu lato. Bull World Health Organ 77(3):230–234PubMedGoogle Scholar
  5. 5.
    Chandre F, Darriet F, Manguin S, et al (1999) Pyrethroid cross resistance spectrum among populations of Anopheles gambiae s.s. from Côte-d’Ivoire. J Am Mosq Control Assoc 15(1):53–59PubMedGoogle Scholar
  6. 6.
    Chandre F, Manguin S, Brengues C, et al (1999) Current distribution of a pyrethroid resistance gene (kdr) in Anopheles gambiae complex from west Africa and further evidence for reproductive isolation of the Mopti form. Parassitologia 41(1–3):319–322PubMedGoogle Scholar
  7. 7.
    Choumara R, Ricosse J, Hamon D, et al (1959) Le paludisme dans la zone pilote antipaludique de Bobo-Dioulasso, Haute Volta — 4e partie: Épidémiologie du paludisme dans les zones traitées et non traitées. Cah Orstom, 99 pGoogle Scholar
  8. 8.
    Collins FH, Mendez MA, Rasmussen MO, et al (1987) A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Am J Trop Med Hyg 37(1):37–41PubMedGoogle Scholar
  9. 9.
    Coluzzi M, Petrarca V, Di Deco MA (1985) Chromosomal inversion intergradation in incipient speciation in Anopheles gambiae. Boll Zool 52:45–63CrossRefGoogle Scholar
  10. 10.
    Corbel V, N’Guessan R, Brengues C, et al (2007) Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa. Acta Trop 101(3):207–216. Epub 2007 Feb 12PubMedCrossRefGoogle Scholar
  11. 11.
    Dabiré KR, Diabaté A, Djogbenou L, et al (2008) Dynamics of multiple insecticide resistance in the malaria vector Anopheles gambiae in a rice growing area in South-Western Burkina Faso. Malaria J 7: 188CrossRefGoogle Scholar
  12. 12.
    Darriet F, Robert V, Tho Vien N, Carnevale P (1984) Évaluation de l’efficacité sur les vecteurs du paludisme de la perméthrine en imprégnation de moustiquaires intactes et trouées. Document miméographié OMS WHO/VBC/84 899 et WHO/MAL/84, 1008, 20 pGoogle Scholar
  13. 13.
    Détinova TS (1963) Méthodes à appliquer pour classer par groupe d’âge les diptères présentant une importance médicale. OMS. Ser Monogr 47:220Google Scholar
  14. 14.
    Diabaté A, Baldet T, Chandre F, et al (2002) The role of agricultural use of insecticides in resistance to pyrethroids in Anopheles gambiae s.l. in Burkina Faso. Am J Trop Med Hyg 67(6):617–622PubMedGoogle Scholar
  15. 15.
    Elissa N, Mouchet J, Rivière F, et al (1993) Resistance of Anopheles gambiae s.s. to pyrethroids in Côte-d’Ivoire. Ann Soc Belg Med Trop 73(4):291–294PubMedGoogle Scholar
  16. 16.
    Etang J, Manga L, Chandre F, et al (2003) Insecticide susceptibility status of Anopheles gambiae s.l. (Diptera: Culicidae) in the Republic of Cameroon. J Med Entomol 40(4):491–497PubMedCrossRefGoogle Scholar
  17. 17.
    Favia G, della Torre A, Bagayoko M, et al (1997) Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation. Insect Mol Biol 6(4):377–383PubMedCrossRefGoogle Scholar
  18. 18.
    Gillies MT, Coetzee M (1987) A supplement to the anophelinae of Africa south of the Sahara (Afrotropical region). Publ South Afr Inst Med Res 55:1–139Google Scholar
  19. 19.
    Gimnig JE, Vulule JM, Lo TQ, et al (2003) Impact of permethrin-treated bed nets on entomologic indices in an area of intense year-round malaria transmission. Am J Trop Med Hyg 68(4Suppl):16–22PubMedGoogle Scholar
  20. 20.
    Girod R, Orlandi-Pradines E, Rogier C, Pages F (2006) Malaria transmission and insecticide resistance of Anopheles gambiae (Diptera: Culicidae) in the French military camp of Port-Bouët, Abidjan (Côte-d’Ivoire): implications for vector control. J Med Entomol 43(5):1082–1087PubMedCrossRefGoogle Scholar
  21. 21.
    Goudet J, Raymond M, De Meeüs T, Rousset F (1996) Testing differentiation in diploid populations. Genetics 144(4):1933–1940PubMedGoogle Scholar
  22. 22.
    Lines JD, Zaim M (2000) Insecticide products: treatment of mosquito nets at home. Parasitol Today 16(3):91–92PubMedCrossRefGoogle Scholar
  23. 23.
    Martinez-Torres D, Chandre F, Williamson MS, et al (1998) Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s. Insect Mol Biol 7(2):179–184PubMedCrossRefGoogle Scholar
  24. 24.
    Nahlen BL, Clark JP, Alnwick D (2003) Insecticide-treated bed nets. Am J Trop Med Hyg 68(4 Suppl):1–2PubMedGoogle Scholar
  25. 25.
    Nevill CG, Some ES, Mung’ala VO, et al (1996) Insecticide-treated bednets reduce mortality and severe morbidity from malaria among children on the Kenyan coast. Trop Med Int Health 1(2):139–146PubMedCrossRefGoogle Scholar
  26. 26.
    Ranson H, Jensen B, Vulule JM, et al (2000) Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Mol Biol 9(5):491–497PubMedCrossRefGoogle Scholar
  27. 27.
    Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249Google Scholar
  28. 28.
    Reimer LJ, Tripet F, Slotman M, et al (2005) An unusual distribution of the kdr gene among populations of Anopheles gambiae on the island of Bioko, Equatorial Guinea. Insect Mol Biol 14(6): 683–688PubMedCrossRefGoogle Scholar
  29. 29.
    Rousset F, Raymond M (1995) Testing heterozygote excess and deficiency. Genetics 140(4):1413–1419PubMedGoogle Scholar
  30. 30.
    Scott JA, Brogdon WG, Collins FH (1993) Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Amer Soc Trop Med Hyg 49(4):520–529Google Scholar
  31. 31.
    Stump AD, Atieli FK, Vulule JM, Besansky NJ (2004) Dynamics of the pyrethroid knockdown resistance allele in western Kenyan populations of Anopheles gambiae in response to insecticidetreated bed net trials. Am J Trop Med Hyg 70(6):591–596PubMedGoogle Scholar
  32. 32.
    UNIPHYTO (1997) Sommaire du marché phytosanitaire en Côted’Ivoire. Rapport non publié, Union de la profession phytosanitaire, AbidjanGoogle Scholar
  33. 33.
    Vezenegho SB, Brooke BD, Hunt RH, et al (2009) Malaria vector composition and insecticide susceptibility status in Guinea Conakry, West Africa. Med Vet Entomol 23(4):326–334PubMedCrossRefGoogle Scholar
  34. 34.
    Weill M, Chandre F, Brengues C, et al (2000) The kdr mutation occurs in the Mopti form of Anopheles gambiae s.s. through introgression. Insect Mol Biol 9(5):451–455PubMedCrossRefGoogle Scholar
  35. 35.
    Weir BS, Cockerham CC (1984) Estimating F-stastics for the analysing of population structure. Evolution 38(6):1358–1370CrossRefGoogle Scholar
  36. 36.
    Wondji C, Simard F, Fontenille D (2002) Evidence for genetic differentiation between the molecular forms M and S within the Forest chromosomal form of Anopheles gambiae in an area of sympatry. Insect Mol Biol 11(1):11–19PubMedCrossRefGoogle Scholar
  37. 37.
    World Health Organisation (WHO) (2000) Review of: IR3535; KBR3023; (RS)-Methoprene 20% EC, Pyriproxyfen 0.5% GR; and Lambda-Cyhalothrin 2.5% CS. Report of the fourth WHOPES Working Group Meeting (4–5 December 2000). WHO document WHO/CDS/WHOPES/2001 2Google Scholar
  38. 38.
    Yawson AE, McCall PJ, Wilson MD, Donnelly MJ (2004) Species abundance and insecticide resistance of Anopheles gambiae in selected areas of Ghana and Burkina Faso. Med Vet Entomol 18(4): 372–377PubMedCrossRefGoogle Scholar

Copyright information

© Springer Verlag France 2012

Authors and Affiliations

  1. 1.Laboratoire de génétiqueuniversité de DaloaDaloaCôte-d’Ivoire
  2. 2.Institut de recherche pour le développementAbidjanCôte-d’Ivoire
  3. 3.MIVEGEC (UM1/CNRS 5290/IRD 224)institut de recherche pour le développementMontpellier cedex 05France

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