Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina
The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.
KeywordsZostera marina antioxidant enzyme glutathione S-transferase (GST) temperature enzyme activity
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We are thankful to all of the members of the Marine Ecology Laboratory (College of Marine Life, Ocean University of China) for their experimental and helpful proposals. Without their help, this work cannot go so well.
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