Family-level diversity of extracellular proteases of sedimentary bacteria from the South China Sea

  • Jinyu Yang
  • Yangyang Feng
  • Xiulan Chen
  • Binbin Xie
  • Yuzhong Zhang
  • Mei Shi
  • Xiying ZhangEmail author


Protease-producing bacteria and their extracellular proteases are key players in degrading organic nitrogen to drive marine nitrogen cycling and yet knowledge on both of them is still very limited. This study screened protease-producing bacteria from the South China Sea sediments and analyzed the diversity of their extracellular proteases at the family level through N-terminal amino acid sequencing. Results of the 16S rRNA gene sequence analysis showed that all screened protease-producing bacteria belonged to the class Gammaproteobacteria and most of them were affiliated with different genera within the orders Alteromonadales and Vibrionales. The Nterminal amino acid sequence analysis for fourteen extracellular proteases from fourteen screened bacterial strains revealed that all these proteases belonged to the M4 family of metalloproteases or the S8 family of serine proteases. This study presents new details on taxa of marine sedimentary protease-producing bacteria and types of their extracellular proteases, which will help to comprehensively understand the process and mechanism of the microbial enzymatic degradation of marine sedimentary organic nitrogen.

Key words

protease-producing bacteria diversity extracellular proteases protease families N-terminal amino acid sequencing South China Sea 


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Copyright information

© Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jinyu Yang
    • 1
    • 4
  • Yangyang Feng
    • 1
  • Xiulan Chen
    • 1
  • Binbin Xie
    • 1
  • Yuzhong Zhang
    • 1
    • 2
    • 3
  • Mei Shi
    • 1
  • Xiying Zhang
    • 1
    Email author
  1. 1.State Key Laboratory of Microbial Technology, Marine Biotechnology Research CenterShandong UniversityQingdaoChina
  2. 2.Laboratory for Marine Biology and BiotechnologyPilot National Laboratory for Marine Science and Technology (Qingdao)QingdaoChina
  3. 3.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  4. 4.Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources ProcessingMinistry of Agriculture and Rural AffairsJinanChina

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