An ocean acidification-simulated system and its application in coral physiological studies
Due to the elevated atmospheric carbon dioxide, ocean acidification (OA) has recently emerged as a research theme in marine biology due to an expected deleterious effect of altered seawater chemistry on calcification. A system simulating future OA scenario is crucial for OA-related studies. Here, we designed an OA-simulated system (OASys) with three solenoid-controlled CO2 gas channels. The OASys can adjust the pH of the seawater by bubbling CO2 gas into seawaters via feedback systems. The OASys is very simple in structure with an integrated design and is new-user friendly with the instruction. Moreover, the OASys can monitor and record real-time pH values and can maintain pH levels within 0.02 pH unit. In a 15-d experiment, the OASys was applied to simulate OA in which the expected target pH values were 8.00, 7.80 and 7.60 to study the calcifying response of Galaxea fascicularis. The results showed daily mean seawater pH values held at pH 8.00±0.01, 7.80±0.01 and 7.61±0.01 over 15 d. Correspondingly, the coral calcification of G. fascicularis gradually decreased with reduced pH.
Key wordsocean acidification OASys coral Galaxea fascicularis
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- Barry J P, Lovera C, Okuda C, et al. 2008. A gas-controlled aquarium system for ocean acidification studies. In: OCEANS 2008-MTS/IEEE Kobe Techno-Ocean. Kobe, Japan: IEEE, 1–5Google Scholar
- Buddemeier R W, Jokiel P L, Zimmerman K M, et al. 2008. A modeling tool to evaluate regional coral reef responses to changes in climate and ocean chemistry. Limnology and Oceanography: Methods, 6(9): 395–411, doi: 10.4319/lom.2008.6.395Google Scholar
- Comeau S, Carpenter R C, Edmunds P J. 2013a. Response to coral reef calcification: carbonate, bicarbonate and proton flux under conditions of increasing ocean acidification. Proceedings of the Royal Society B: Biological Sciences, 280(1764): 20131153, doi: 10.1098/rspb.2013.1153CrossRefGoogle Scholar
- Dickson A G, Sabine C L, Christian J R. 2007. Guide to best practices for ocean CO2 measurements. Sidney, Canada: North Pacific Marine Science OrganizationGoogle Scholar
- Fangue N A, O'Donnell M J, Sewell M A, et al. 2010. A laboratorybased, experimental system for the study of ocean acidification effects on marine invertebrate larvae. Limnology and Oceanography: Methods, 8(8): 441–452, doi: 10.4319/lom.2010.8.441Google Scholar
- Gattuso J P, Allemand D, Frankignoulle M. 1999. Photosynthesis and calcification at cellular, organismal and community levels in coral reefs: a review on interactions and control by carbonate chemistry. Integrative and Comparative Biology, 39(1): 160–183Google Scholar
- Hofmann G E, Barry J P, Edmunds P J, et al. 2010. The effect of ocean acidification on calcifying organisms in marine ecosystems: an organism-to-ecosystem perspective. Annual Review of Ecology, Evolution, and Systematics, 41: 127–147, doi: 10.1146/annurev. ecolsys.110308.120227CrossRefGoogle Scholar
- McGraw C M, Cornwall C E, Reid M R, et al. 2010. An automated pHcontrolled culture system for laboratory-based ocean acidification experiments. Limnology and Oceanography: Methods, 8(12): 686–694, doi: 10.4319/lom.2010.8.0686Google Scholar
- Zheng Xinqing, Li Yuanchao, Lin Rongcheng, et al. 2013. Coral reef conservation and restoration in Mainland China. Malysian Journal of Science, 32(SI): 221–238Google Scholar