Effects of virus infection on expression of cell cycle regulatory proteins in the unicellular marine algae Emiliania huxleyi
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The authors have investigated the biochemical events by which marine algal virus infection induces cell cycle arrest. The key G2/M-phase regulatory proteins are analyzed by immunobloting in unicellular Emiliania huxleyi, suggesting that virus induced cell cycle arrest is related with virus’s effect on cyclins and cyclin dependent kinases. E. huxleyi virus (EhV) represses Cdc2/cyclinB complex activity by inhibiting the activity of Cdc2 kinase in a phosphorylation-related manner, blocking host cells G2/M checkpoint. Dephosphorylated / inactive Cdc25C combined with up-regulation of Wee1 expression at early infect period appears to be important mechanisms by which EhV represses Cdc2/cyclinB complex activity that is required for entry into M phase. This study has allowed us to confirm that algal virus infection leads to selective activation or inhibition of certain cell-cycle factors, which may play a significant role in establishing a more efficient environment for viral gene expression and DNA replication.
Key wordsmarine algal virus cell cycle regulators expression Emiliania huxleyi
- Advani S J, Brandimarti R, Weichselbaum R R, et al. 2000. The Disappearance of cyclins A and B and the increase in activity of the G2/M-phase cellular kinase cdc2 in herpes simplex virus 1-infected cells require expression of the α22/US1.5 and UL13 viral genes. J Virol, 74: 8–15CrossRefGoogle Scholar
- Lin Senjie, Carpenter E J, Chang J. 1996. Detection of p34cdc2- and cyclin B-like proteins in Dunaliella tertiolecta (Chlorophyceae). Mar Biol, 125(3): 603–610Google Scholar
- Liu Feng, Rothblum-Oviatt C, Ryan C E, et al. 1999. Overproduction of human Myt1 Kinase induces a G2 cell cycle delay by interfering with the intracellular trafficking of Cdc2-Cyclin B1 complexes. Mol Cell Biol, 19: 5113–5123Google Scholar
- Wahlund T M, Hadaegh A R, Clark R, et al. 2005. Emiliania huxleyi CCMP1516 main genome assembly v1.0. http://genome.jgi psf.org/Emihu1/Emihu1.home.html