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An integrative taxonomic study reveals carychiid microsnails of the troglobitic genus Zospeum in the Eastern and Dinaric Alps (Gastropoda, Ellobioidea, Carychiinae)

  • Thomas InäbnitEmail author
  • Adrienne Jochum
  • Marian Kampschulte
  • Gunhild Martels
  • Bernhard Ruthensteiner
  • Rajko Slapnik
  • Claudia Nesselhauf
  • Eike Neubert
Original Article
  • 38 Downloads

Abstract

The minute snails of the troglobitic genus Zospeum are known from the southeastern Alps through the Dinarides and from the Pyrenees to the Cantabrian Mountains. The majority of Zospeum has been described from the northernmost region of Southeast Europe, the geographical focus of our study. The taxonomic value of the few available morphological shell characters has been debated for nearly as long as the genus is known. Recent results of genetic studies questioned the established taxonomic system based on morphological characters in the 1970s, with one species (Z. isselianum) appearing to be polyphyletic. Here, we present a comprehensive revision of Zospeum from the Alpine and Dinaride regions, using an integrative approach including genetic methods, morphometry, X-ray micro computer tomography (micro-CT), and SEM. We reveal 25 species and describe 5 new to science. Genetic analysis separated the genus into five clades, strongly challenging the previously valid taxonomic system and revealing several species polyphyletic. The various methods used for morphological characterization often proved useful in separating species showing high incidence of cryptic speciation in the genus. Radular investigation, using SEM, uncovered new insights about the taxonomic value of the configuration of the radular ribbon within Zospeum as well as Zospeum’s dentitional affinity within the Ellobioidea.

Keywords

Troglobitic microsnails Dinarides Shell variability Subterranean ecology Molecular phylogenetics Morphometrics Computer tomography Microgastropods 

Abbreviations

Shell characters

(see Fig. 2)

AH

Aperture height

AW

Aperture width

CJL

Length of columellar junction between shell and aperture

D1

Diameter of first whorl

LH

Height of last whorl

LW

Width of last whorl

SA

Spire angle

SH

Shell height

SW

Shell width

Material is housed in the following collections:

AJC

Adrienne Jochum Collection, Kelkheim, Germany

ANSP

Academy of Natural Sciences, Philadelphia, Pa., USA

NHMUK

Natural History Museum, London, UK

GN

Gianbattista Nardi Collection, Gussago, Brescia, Italy

MCSMNH

Malacological Collection of the Slovenian Museum of Natural History (former CSR SASA, MZBI and SMNH) Ljubljana, Slovenia

MCSNTS

Museum of Natural History of Trieste, Trieste, Italy

NHMW

Naturhistorisches Museum Wien, Wien, Austria

NMBE

Naturhistorisches Museum der Burgergemeinde Bern, Bern, Switzerland

RS

Rajko Slapnik Collection, Kamnik, Slovenia

SMF

Senckenberg Forschungsinstitut und Naturmuseum, Frankfurt am Main, Germany

WdM

Willy De Mattia Collection, Muggia, Italy

MP

Massimo Prodan Collection, Trieste, Italy

ZRC SAZU

The Slovenian Academy of Sciences and Arts (encompasses Jovan Hadži Biological Institute (BIJH)) Ljubljana, Slovenia

ZSM

Zoologische Staatssammlung München

Notes

Acknowledgements

We are grateful to the collection managers and curators, who generously gave access to the collections under their care: Anita Eschner (Naturhistorisches Museum, Vienna), Ronald Janssen (Research Institute Senckenberg, Frankfurt am Main), Jonathan Ablett (Natural History Museum, London), and Matjaž Kuntner (Center for Scientific Research of the Slovenian Academy of Sciences and Arts, Ljubljana). We especially thank those who patiently imaged the type material including Katharina Jaksch-Mason (NHMW), Paulo Albano (NHMW), Sara-Maria Schnedl (NHMW), Sigrid Hof (SMF), and Estée Bochud (NMBE). We are also indebted to those who generously sent us precious specimens from their private collections including Massimo Prodan, Willy De Mattia, and Gianbattista Nardi. For many insightful discussions and support, we especially thank Anita Eschner, Ruud Bank, Alexander Weigand, Beat Pfarrer, and Massimo Prodan. We acknowledge Tonći Rađa for his kind assistance interpreting Croatian cave names and locality data. For material and GPS collection data, we thank Roman Ozimec and the Croatian Biospeleological Society as well as Sara-Maria Schnedl for her help in translating Italian texts. We gratefully acknowledge Thomas Neubauer who kindly transported borrowed material back and forth to Vienna. We also thank Manfred Ruppel (ret. Goethe University Frankfurt) for his insights and help with the SEM and Annette Klussmann-Kolb for her generous support. For creation of the video of Z. obesum, we thank Jean Martels and Henning Heckmann (Justus Liebig University Computer Center, Giessen). This work was made possible in part by grants to AJ from the Malacological Society of London for SEM resources and from the SYNTHESYS Project http://www.synthesys.info/, which enabled AJ to spend weeks on end studying the NHMW collection. The SYNTHESYS Project is financed by the European Community Research Infrastructure Action under the FP7 “Capacities” Program.

Supplementary material

13127_2019_400_Fig11_ESM.png (790 kb)
Supplementary Figure S1

Overall morphometric analyses. From top to bottom: measurement-based PCA, geometric PCA, CVA. (PNG 789 kb)

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High Resolution Image (TIF 7811 kb)
13127_2019_400_Fig12_ESM.png (537 kb)
Supplementary Figure S2

Morphometric analyses of the Z. spelaeum clade. From top to bottom: measurement-based PCA, geometric PCA, CVA. (PNG 537 kb)

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High Resolution Image (TIF 6203 kb)
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Supplementary Figure S3

Morphometric results of the Z. alpestre clade. From top to bottom: measurement-based PCA, geometric PCA, CVA. (PNG 471 kb)

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High Resolution Image (TIF 5776 kb)
13127_2019_400_Fig14_ESM.png (355 kb)
Supplementary Figure S4

Morphometric results of the Z. obesum clade. From top to bottom: measurement-based PCA, geometric PCA, CVA. (PNG 355 kb)

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High Resolution Image (TIF 5292 kb)
13127_2019_400_Fig15_ESM.png (523 kb)
Supplementary Figure S5

Morphometric results of the Z. pretneri clade. From top to bottom: measurement-based PCA, geometric PCA, CVA. (PNG 523 kb)

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High Resolution Image (TIF 6493 kb)
13127_2019_400_Fig16_ESM.png (546 kb)
Supplementary Figure S6

Morphometric results of the Z. frauenfeldii clade. From top to bottom: measurement-based PCA, geometric PCA, CVA. (PNG 546 kb)

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High Resolution Image (TIF 8475 kb)
13127_2019_400_Fig17_ESM.png (525 kb)
Supplementary Figure S7

Morphometric results of Z. globosum, sorted by populations. From top to bottom: measurement-based PCA, geometric PCA, CVA. (PNG 525 kb)

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High Resolution Image (TIF 6412 kb)
13127_2019_400_Fig18_ESM.png (1.2 mb)
Supplementary Figure S8

Distribution of species within the Z. spelaeum clade. (PNG 1276 kb)

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High Resolution Image (TIF 2132 kb)
13127_2019_400_Fig19_ESM.png (1 mb)
Supplementary Figure S9

Distribution of species within the Z. alpestre clade. (PNG 1027 kb)

13127_2019_400_MOESM9_ESM.tif (1.9 mb)
High Resolution Image (TIF 1896 kb)
13127_2019_400_Fig20_ESM.png (910 kb)
Supplementary Figure S10

Distribution of species within the Z. obesum clade. (PNG 909 kb)

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High Resolution Image (TIF 1693 kb)
13127_2019_400_Fig21_ESM.png (1.5 mb)
Supplementary Figure S11

Distribution of species within the Z. pretneri clade. (PNG 1584 kb)

13127_2019_400_MOESM11_ESM.tif (2.4 mb)
High Resolution Image (TIF 2414 kb)
13127_2019_400_Fig22_ESM.png (2.4 mb)
Supplementary Figure S12

Distribution of species within the Z. frauenfeldii clade. (PNG 2445 kb)

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High Resolution Image (TIF 28018 kb)
13127_2019_400_Fig23_ESM.png (2.7 mb)
Supplementary Figure S13

Distribution of species that were not assigned to any clade and of specimens with unknown identity. (PNG 2715 kb)

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High Resolution Image (TIF 3674 kb)
13127_2019_400_Fig24_ESM.png (2.6 mb)
Supplementary Figure S14

Scanning electron micrographs (SEM) showing superficial microstructure on Zospeum shells. (a) Zospeum speleaum, CSRSASA 3983ª, Skocjanske jama, pitting on protoconch. — (b) Zospeum spelaeum, MCSMNH 31286, Mačkovica jama, Interconnected rows of pitting on protoconch. — (c) Zospeum amoenum, MCSMNH 21675, Konečka zijalka, rows of pits interspersed with irregular smooth zones on protoconch; (d) ditto, interconnected rows of pits on teleoconch. — (e) Zospeum isselianum, MCSMNH 37013, Turjeva jama, distinct regular rows of pits interspersed by regular non-pitted zones on protoconch; (f) ditto, distinct spiral striae of interconnected pits and raised revolving lines of microstructure on teleoconch. — (g) Zospeum kupitzense, SMNH 3291, Ložekarjeva jama, pitting on protoconch; (h) ditto, incised suture and more or less regular bands of interconnected pits on teleoconch. — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 2642 kb)

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High Resolution Image (TIF 14590 kb)
13127_2019_400_Fig25_ESM.png (1.2 mb)
Supplementary Figure S15

Scanning electron micrographs (SEM) showing superficial microstructure on Zospeum shells. (a) Zospeum obesum, NMBE 553409, Krška jama, interconnected pits on protoconch; (b) ditto, distinct spiral striae on teleoconch. — (c) Zospeum exiguum, MCSMNH 39012, Križna jama, protoconch with dense pitting and organic debris; (d) ditto, distinct spiral striae on the penultimate whorl; — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 1210 kb)

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High Resolution Image (TIF 7633 kb)
13127_2019_400_Fig26_ESM.png (2.2 mb)
Supplementary Figure S16

Scanning electron micrographs (SEM) showing superficial microstructure on Zospeum shells. (a) Zospeum globosum, NHMW 111543, Cavernetta di Vobarno, pitted protoconch; (b) ditto, sprial striae of interconnected pits and eroded rib-like microstructure on teleoconch. — (c) Zospeum subobesum, NMBE 553326, Tounjčica Cave, pitting on protoconch; (d) ditto, spiral striae of interconnected pits on teleoconch. — (e) Zospeum frauenfeldii, NMBE 553388, Podpeška jama, protoconch showing dense microstructure of interconnected pits; (f) ditto, revolving lines of reticulate structure on 2nd whorl; (g) ditto, pronounced ribbing on teleoconch. — (h) Zospeum subobesum, NMBE 553326, Tounjčica Cave, teleoconch showing partial costate structure at junction of penultimate and ultimate whorls. — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 2205 kb)

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High Resolution Image (TIF 14249 kb)
13127_2019_400_Fig27_ESM.png (3.1 mb)
Supplementary Figure S17

Scanning electron micrographs (SEM) showing microstructure on Zospeum radulae. (a) Zospeum exiguum, NMBE 553384, Križna jama, long, tapered-type of radular ribbon; (b) ditto, bicuspid crowns bearing semi-detached endocones; (c) ditto, overview showing tooth and basal plate alignment; (d) ditto, biscuspid laterals (right side) and transitional teeth bearing four-pointed cusps (left side). — (e) Zospeum frauenfeldii, NMBE 553388, Podpeška jama, serrated marginal crowns atop of concave basal plates; (f) ditto, rachidian tooth (middle column) flanked by irregular lateral teeth; (g) ditto, row of lateral teeth showing distinct grooves down the mesocone; (h) ditto, tapered-type radular ribbon with attenuated triangular base. — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 3140 kb)

13127_2019_400_MOESM17_ESM.tif (10 mb)
High Resolution Image (TIF 10240 kb)
13127_2019_400_Fig28_ESM.png (3.1 mb)
Supplementary Figure S18

Scanning electron micrographs (SEM) showing microstructure on Zospeum radulae. (a) Zospeum isselianum, NMBE 553389, Turjeva jama, tricuspid lateral teeth; (b) ditto, marginal teeth; (c) ditto, rachidian and lateral teeth. — (d–e) Zospeum amoenum, NMBE 553377, Konečka zijalka, crowns showing mesocones with deep median grooves and semi-detached endocones. — (f) Zospeum obesum, NMBE 553409, Krška jama, very long, tapered-type ribbon with pointed or obtuse ends; (g) ditto, stressed and broken lateral crowns; (h) ditto, worn crowns of transitional teeth. — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 3123 kb)

13127_2019_400_MOESM18_ESM.tif (13.9 mb)
High Resolution Image (TIF 14250 kb)
13127_2019_400_Fig29_ESM.png (2.4 mb)
Supplementary Figure S19

Scanning electron micrographs (SEM) showing microstructure on Zospeum radulae. (a) Zospeum pretneri, NMBE 553290, Gornja Cerovačka pećina, tiny rachidian tooth and wing-like basal plates; (b) ditto, centrally grooved and partially bulged crowns of lateral teeth; (c) ditto, tricuspid teeth with wing-like basal plates; (d) ditto, consecutive rows of tricuspid crowns and wing-like basal plates. — (e) Zospeum kupitzense, NMBE 553393, Ložekarjeva jama, very long, anteriorly-tapered ribbon with straight-edged-type base; (f) ditto, long and sharply pointed transitional crowns. — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 2407 kb)

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High Resolution Image (TIF 11274 kb)
13127_2019_400_Fig30_ESM.png (2.3 mb)
Supplementary Figure S20

Scanning electron micrographs (SEM) showing microstructure on Zospeum radulae. (a) Zospeum spelaeum, MCSMNH 36841a, Postojnska jama, long and tapered-type ribbon with obtuse ends; (b) ditto, right lateral teeth; (c) ditto, transitional teeth; (d) ditto, marginal teeth. — (e) Zospeum spelaeum, NMBE 553311, Velika Pasica, very long and tapered-type ribbon with pointed or obtuse ends; (f) ditto, overview of middle radular section and concave basal plates. — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 2405 kb)

13127_2019_400_MOESM20_ESM.tif (11 mb)
High Resolution Image (TIF 11240 kb)
13127_2019_400_Fig31_ESM.png (2.3 mb)
Supplementary Figure S21

Scanning electron micrographs (SEM) showing microstructure on Zospeum radulae. (a) Zospeum spelaeum, NMBE 553311, Velika Pasica, grooved and wrinkled, marble-like surface texture; (b) ditto, grooved transitional teeth; (c) ditto, marginal teeth. — (d) Zospeum subobesum, NMBE 553326, Tounjčica, very long, anteriorly-tapered ribbon with straight-edged-type base; (e) ditto, marginal teeth; (f) ditto, transitional teeth. — Magnification varies for each perspective, see scale bars; all Figs taken by M. Ruppel, formerly Goethe University Frankfurt am Main. (PNG 2317 kb)

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High Resolution Image (TIF 11083 kb)
13127_2019_400_MOESM22_ESM.doc (306 kb)
ESM 1 (DOC 306 kb)

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Copyright information

© Gesellschaft für Biologische Systematik 2019

Authors and Affiliations

  • Thomas Inäbnit
    • 1
    • 2
    Email author
  • Adrienne Jochum
    • 1
    • 2
  • Marian Kampschulte
    • 3
  • Gunhild Martels
    • 4
  • Bernhard Ruthensteiner
    • 5
  • Rajko Slapnik
    • 6
  • Claudia Nesselhauf
    • 7
  • Eike Neubert
    • 1
    • 2
  1. 1.Natural History Museum of the Burgergemeinde BernBernSwitzerland
  2. 2.Institute of Ecology and EvolutionUniversity of BernBernSwitzerland
  3. 3.Center for Radiology, Department of RadiologyUniversitätsklinikum Giessen und Marburg GmbH-Standort GiessenGiessenGermany
  4. 4.Department of Experimental RadiologyJustus-Liebig University Giessen, Biomedical Research Center Seltersberg (BFS)GiessenGermany
  5. 5.Zoologische Staatssammlung MünchenMunichGermany
  6. 6.KamnikSlovenia
  7. 7.Institute for Ecology, Evolution & DiversityGoethe-UniversityFrankfurt/MGermany

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