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Organisms Diversity & Evolution

, Volume 16, Issue 3, pp 597–611 | Cite as

Who lives where? Molecular and morphometric analyses clarify which Unio species (Unionida, Mollusca) inhabit the southwestern Palearctic

  • Elsa Froufe
  • Duarte V. Gonçalves
  • Amílcar Teixeira
  • Ronaldo Sousa
  • Simone Varandas
  • Mohamed Ghamizi
  • Alexandra Zieritz
  • Manuel Lopes-Lima
Original Article

Abstract

Many doubts still exist about which freshwater mussel Unio species inhabit Northwest Africa. While some authors refer to the presence of Unio delphinus in the Atlantic North African basins of Morocco, a recent International Union for Conservation of Nature (IUCN) assessment performed on Moroccan Unio species, recognised the existence of a distinct species, Unio foucauldianus, with a critically endangered conservation status. The present study delivered new genetic, morphological, and geographical distribution data on two Unio species (i.e. U. delphinus and U. foucauldianus) greatly increasing the almost non-existent data on these taxa. Bayesian phylogenetic analysis revealed two highly supported geographically concordant clades, which diverged by 3.2 ± 0.6 % (uncorrected p distance): the first distributed across Iberia and corresponding to U. delphinus, and the second distributed across Morocco, corresponding to U. foucauldianus. These results were corroborated by the analysis of ten newly developed microsatellite loci as well as shell morphometry. We suggest that the IUCN critically endangered conservation status of U. foucauldianus should be revised and probably down-listed since its actual distribution is much wider than previously described. Phylogenetic relationships with the other Unio species were resolved, showing that U. delphinus and U. foucauldianus fall inside the pictorum lineage. The estimated molecular rate reported herein (0.265 ± 0.06 % per million years) represents the first for the Unionida and could be used as a reference in future studies.

Keywords

Freshwater molluscs Unionidae Microsatellites Messinian Molecular clock 

Notes

Acknowledgements

The authors wish to thank the two anonymous reviewers for helpful remarks and suggestions that improved the quality of the manuscript. Financial support was provided by Portuguese Foundation for Science and Technology (FCT) project PTDC/AAC-AMB/117688/2010 and by Mohamed bin Zayed Species Conservation Fund (reference 15256799). Official capture and sampling licenses were issued by the following agencies: (1) Junta de Castilla y León (Consejería de Fomento y Medio Ambiente EP/CYL/331/2012, de 17 de Abril de 2012); (2) Junta de Andalucia (Consejería de Medio Ambiente-SGYB/FOA/AFR/SFS, de 17 de Julio de 2012); and (3) Université Cadi Ayyad (Faculté des Sciences, Semlalia, Marrakech, Maroc).

Supplementary material

13127_2016_262_MOESM1_ESM.docx (28 kb)
Table S1 (DOCX 27 kb)
13127_2016_262_MOESM2_ESM.docx (17 kb)
Table S2 (DOCX 17 kb)
13127_2016_262_MOESM3_ESM.docx (19 kb)
Table S3 (DOCX 19 kb)
13127_2016_262_MOESM4_ESM.docx (15 kb)
Table S4 (DOCX 14 kb)

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Copyright information

© Gesellschaft für Biologische Systematik 2016

Authors and Affiliations

  • Elsa Froufe
    • 1
  • Duarte V. Gonçalves
    • 2
    • 3
    • 4
  • Amílcar Teixeira
    • 5
  • Ronaldo Sousa
    • 1
    • 6
  • Simone Varandas
    • 7
  • Mohamed Ghamizi
    • 8
  • Alexandra Zieritz
    • 9
  • Manuel Lopes-Lima
    • 1
  1. 1.CIIMAR/CIMAR-Interdisciplinary Centre of Marine and Environmental ResearchUniversity of PortoPortoPortugal
  2. 2.CIBIO/InBIO-Centro de Investigação em Biodiversidade e Recursos Genéticos da Universidade do PortoVairãoPortugal
  3. 3.Departamento de Biologia da Faculdade de Ciências da Universidade do PortoPortoPortugal
  4. 4.Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra)BarcelonaSpain
  5. 5.CIMO-ESA-IPB-Mountain Research Centre, School of AgriculturePolytechnic Institute of BragançaBragançaPortugal
  6. 6.CBMA-Centre of Molecular and Environmental Biology, Department of BiologyUniversity of MinhoBragaPortugal
  7. 7.CITAB-UTAD-Centre for Research and Technology of Agro-Environment and Biological Sciences, Forestry DepartmentUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  8. 8.Muséum d’Histoire Naturelle de Marrakech, Faculté des SciencesUniversité Cadi AyyadSemlaliaMorocco
  9. 9.School of GeographyUniversity of Nottingham Malaysia CampusJalan BrogaMalaysia

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