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Organisms Diversity & Evolution

, Volume 13, Issue 1, pp 67–76 | Cite as

Biogeographic patterns in the chromosomal distribution of a satellite DNA in the banded tetra Astyanax fasciatus (Teleostei: Characiformes)

  • Karine Frehner KavalcoEmail author
  • Rubens Pazza
  • Karina de Oliveira Brandão
  • Lurdes Foresti de Almeida-Toledo
Original Article

Abstract

The As-51 satellite DNA is a transposon-like sequence formerly described for arthropods and physically identifiable by fluorescent in situ hybridization. In the present work, we describe the occurrence of this sequence, as well the C-banding and karyotype composition, in populations of the group Astyanax fasciatus from Mogi-Guaçu (Araras-SP), Paranapanema (Angatuba and Pilar do Sul-SP), Ribeira de Iguape (Sete Barras-SP) and Tietê (Indaiatuba and Salesópolis-SP) river basins. The specimens from Sete Barras (10 M + 20SM + 12ST + 6A) and Araras (8 M + 22SM + 12ST + 6A) have 2n = 48 chromosomes. The samples from Angatuba, Pilar do Sul and Indaiatuba presented 2n = 46 chromosomes (12 M + 20SM + 10ST + 4A). The individuals collected in Salesópolis showed three cytotypes, bearing 2n = 46 (12 M + 20SM + 10ST + 4A), 2n = 48 (8 M + 22SM + 12ST + 6A) and 2n = 50 (8 M + 16SM + 14ST + 12A). C-banding revealed large heterochromatic blocks at terminal chromosomal regions in all populations and/or cytotypes. All analyzed populations have conspicuous blocks carrying the As-51 satellite DNA, although the number of chromosomes bearing this repetitive sequence was variable among them. Such differences were not related to the diploid number of individuals, but rather to a biogeographic pattern. Aspects of the karyotype evolution and distribution of this sequence in distinct populations are discussed.

Keywords

Astyanax fasciatus Molecular cytogenetics Karyotypic evolution satDNA As-51 

Notes

Acknowledgments

The authors wish to thank Malabarba LR and Bertaco V, for the taxonomical identification; Lopes CE for helping with the samples; Smarzaro RS for helping with the GIS software; and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for the financial support.

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Copyright information

© Gesellschaft für Biologische Systematik 2012

Authors and Affiliations

  • Karine Frehner Kavalco
    • 1
    Email author
  • Rubens Pazza
    • 1
  • Karina de Oliveira Brandão
    • 2
  • Lurdes Foresti de Almeida-Toledo
    • 3
  1. 1.Laboratório de Genética Ecológica e Evolutiva, Instituto de Ciências Biológicas e da SaúdeUniversidade Federal de ViçosaRio ParanaíbaBrazil
  2. 2.Université Libre de BruxellesBrusselsBelgium
  3. 3.Universidade de São Paulo. Instituto de BiociênciasSão PauloBrazil

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