Organisms Diversity & Evolution

, Volume 12, Issue 4, pp 387–402 | Cite as

Species status and population structure of mussels (Mollusca: Bivalvia: Mytilus spp.) in the Wadden Sea of Lower Saxony (Germany)

  • Georg Steinert
  • Thomas Huelsken
  • Gabriele Gerlach
  • Olaf R. P. Bininda-Emonds
Original Article

Abstract

Three species of mussel (genus Mytilus) occur in Europe: M. edulis (Linnaeus 1758), M. galloprovincialis (Lamarck 1819) and M. trossulus (Gould, Boston Society of Natural History 3: 343–348, 1850). Although these species are indigenous to the North Sea, the Mediterranean and the Baltic Sea, respectively, they form an extended patchy species complex along the coasts of Europe (“the Mytilus edulis complex”) and are able to hybridize where their distributions overlap. Recent studies examining the taxonomic status and genetic composition of Mytilus populations in the Netherlands and the British Isles have revealed introgressive hybridization processes within this species complex, with hints of an invasion of nonindigenous M. galloprovincialis into the North Sea. Furthermore, an extensive international mussel fishery industry in Europe (i.e., Great Britain, the Netherlands, Denmark, and Germany) is also in discussion for a possibly anthropogenically induced bioinvasion of nonindigenous Mytilus traits into the Wadden Sea area. Although it is assumed that the Wadden Sea of Germany comprises M. edulis only, this has never been confirmed in a molecular genetic study. To assess the situation for the Wadden Sea of Lower Saxony, we conducted the first molecular study of the Mytilus genus in the region. Taxonomic identification of 504 mussels from 13 intertidal mussel banks using the nDNA marker Me15/16 revealed a population composition of 99% M. edulis and 1% M. edulis X M. galloprovincialis hybrids. Hence, the Wadden Sea population is unaffected by range expansion of nonindigenous Mytilus traits. The genetic structure of the M. edulis populations was investigated using the phylogenetic and population genetics analyses of the mitochondrial DNA cytochrome-c-oxidase subunit I (COI) and the first variable domain of the control region (VD1), which were sequenced for >120 female individuals. These results showed a heterogeneous, panmictic population due to unrestricted gene flow. This can be attributed to extensive larval dispersal linked to the tidal circulation system in the back barrier basins of the Wadden Sea.

Keywords

Bioinvasion Haplotype-networks Hybridization Mytilus edulis-complex Panmixia Phylogeny Population structure 

Notes

Acknowledgments

We thank two anonymous reviewers for the improving comments and suggestions. All sampling was undertaken with the consent of the “Nationalparkverwaltung Niedersächsisches Wattenmeer”, for whom we thank for their assistance. In particular, we thank Alexandra Markert, Dr. Marc Herlyn, Dr. Gerald Millat and Dr. Uwe Walter for their advice and support. Additional sampling trips were made possible through the Dr. Achim Wehrmann (Working Group Actuopalaeontology, Senckenberg am Meer) and the “Nationalparkverwaltung Niedersächsisches Wattenmeer” in cooperation with the ICBM-Terramare. Technical support during the sequencing process was kindly provided by Prof. Dr. Hollmann (Department of Biochemistry I - Receptor Biochemistry, Ruhr University Bochum). Funding assistance was provided in part by the EWE-Stiftung.

Supplementary material

13127_2012_75_MOESM1_ESM.pdf (507 kb)
Supplementary Fig. 1 Mismatch distribution (demographic expansion) of the concatenated mtDNA data set (n = 84) for the total sampling area (i.e., Group 1) (PDF 506 kb)
13127_2012_75_MOESM2_ESM.pdf (2.2 mb)
Supplementary Fig. 2 Haplotype network of the concatenated mtDNA haplotype data set (n = 84). For the associated sampling sites of the consecutive numbering of haplotypes see Supplementary Table 6 (PDF 2226 kb)
13127_2012_75_MOESM3_ESM.docx (42 kb)
Supplementary Table 1 Summary for sequence data obtained from GenBank for various outgroup taxa. Status refers to the position of the inferred haplotype relative to the Wadden Sea clade of M. edulis haplotypes: shared = shared haplotype with M. edulis, within = clusters within clade, outside = clusters outside clade, and outgroup = used to root tree. (DOCX 41 kb)
13127_2012_75_MOESM4_ESM.docx (39 kb)
Supplementary Table 2 Frequencies for the COI, VD1, and combined COI&VD1 sequences from each sampling site and for the pooled locations “west”, “center”, and “east” (DOCX 39 kb)
13127_2012_75_MOESM5_ESM.docx (41 kb)
Supplementary Table 3 Population differentiation showing pairwise (Φ ST) values for the COI coding mtDNA region above the diagonal. Φ ST p-values below diagonal. Total of 10100 permutations (DOCX 40 kb)
13127_2012_75_MOESM6_ESM.docx (41 kb)
Supplementary Table 4 Population differentiation showing pairwise (Φ ST) values for the VD1 mtDNA region above the diagonal. Φ ST P-values below diagonal; 10,100 permutations (DOCX 40 kb)
13127_2012_75_MOESM7_ESM.docx (40 kb)
Supplementary Table 5 Population differentiation showing pairwise (Φ ST) values for the COI&VD1 concatenated mtDNA regions above the diagonal. Φ ST P-values below diagonal; 10,100 permutations (DOCX 40 kb)
13127_2012_75_MOESM8_ESM.docx (45 kb)
Supplementary Table 6 Haplotype data set of the concatenated mtDNA loci with consecutive numbering of haplotypes and their associated sampling sites (DOCX 45 kb)

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Copyright information

© Gesellschaft für Biologische Systematik 2012

Authors and Affiliations

  • Georg Steinert
    • 1
    • 5
  • Thomas Huelsken
    • 2
    • 4
  • Gabriele Gerlach
    • 3
  • Olaf R. P. Bininda-Emonds
    • 1
  1. 1.Faculty V, Institute for Biology and Environmental Sciences (IBU), AG Systematics and Evolutionary BiologyCarl von Ossietzky University OldenburgOldenburgGermany
  2. 2.Department of Biochemistry I—Receptor BiochemistryRuhr University BochumBochumGermany
  3. 3.Faculty V, Institute for Biology and Environmental Sciences (IBU), AG Animal Biodiversity and Evolutionary BiologyCarl von Ossietzky University OldenburgOldenburgGermany
  4. 4.School of Biological SciencesThe University of QueenslandBrisbaneAustralia
  5. 5.ICBM Terramare, Jade InnovationsZentrum, Environmental BiochemistryCarl von Ossietzky University OldenburgWilhelmshavenGermany

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