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Organisms Diversity & Evolution

, Volume 12, Issue 4, pp 377–386 | Cite as

Outdated but established?! Conchologically driven species delineations in microgastropods (Carychiidae, Carychium)

  • Alexander M. Weigand
  • Marie-Carolin Götze
  • Adrienne Jochum
Original Article

Abstract

Valid taxonomic descriptions are paramount in evolutionary biology. Many date back centuries and are based on ambiguous morphological data. Microgastropods, in particular the taxon Carychiidae (Eupulmonata, Ellobioidea), demonstrate a paucity of informative conchological features. However, as exemplified by Carychium mariae Paulucci, 1878, their taxonomic classification is based almost entirely on these few features. Here we investigated the questionable taxonomic status of Carychium mariae combining DNA barcoding, field-emission scanning electron microscopy and conchological data. This taxon occurs in the Southern Alps, where it shows a sympatric distribution with two widely distributed members of CarychiumC. minimum Müller, 1774 and C. tridentatum (Risso, 1826). Our analyses do not support the species status of C. mariae. In contrast, DNA barcoding reveals a monophyletic grouping of C. minimum and C. mariae specimens with averaged intraspecific variability less than 3.2% (barcoding gap for Carychiidae). Hence, C. mariae is treated and should be regarded as a synonym of C. minimum, just representing a different morphotype. The differentiation and monophyletic status of C. tridentatum can be validated by showing an averaged interspecific variability of 5.9% to C. minimum. In general, we are critical of the sole use of conchological characters for microgastropod taxonomy and strongly recommend the implementation of molecular data (e.g., DNA barcoding) to reevaluate established species designations.

Keywords

Microgastropod taxonomy Species descriptions Ellobioidea Carychium mariae 

Notes

Acknowledgements

We thank the Malacological Society of London and the BiK-F Biodiversity and Climate Research Center of the research-funding programme ‘LOEWE—Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz’ of Hesse’s Ministry of Higher Education, Research, and the Arts for their financial support. We also thank Jana Valentinčič, Frank Hardie and Rajko Slapnik for their collecting efforts as well as Manfred Ruppel and Yaron Malkowsky for their support in preparing the FE-SEM photographs. We are grateful to Annette Klussmann-Kolb, Hannah Schweyen and Eugenia Zarza, who provided valuable insights on the conceptual design of this study. Special gratitude goes to Ronald Janssen (Senckenberg Forschungsinstitut und Naturmuseum) and Bruno Dell'Angello for their insights and help in locating the type specimens of C. mariae. We especially wish to thank Simone Cianfanelli (Museo di Storia Naturale dell'Università di Firenze, Sezione di Zoologia de "La Specola") for kindly photographing the syntypes as well as for constructive help in accessing the collection of Marianna Paulucci. We also thank the anonymous reviewers and editors, who provided valuable input on an earlier version of the manuscript.

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Copyright information

© Gesellschaft für Biologische Systematik 2012

Authors and Affiliations

  • Alexander M. Weigand
    • 1
  • Marie-Carolin Götze
    • 1
  • Adrienne Jochum
    • 1
  1. 1.Institute for Ecology, Evolution and DiversityGoethe UniversityFrankfurt am MainGermany

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