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Organisms Diversity & Evolution

, Volume 10, Issue 2, pp 161–172 | Cite as

Red- and yellow-footed tortoises, Chelonoidis carbonaria and C. denticulata (Reptilia: Testudines: Testudinidae), in South American savannahs and forests: do their phylogeographies reflect distinct habitats?

  • Mario Vargas-Ramírez
  • Jérôme Maran
  • Uwe FritzEmail author
Original Article

Abstract

Using sequence data of the mitochondrial cytochrome b gene, we investigated phylogeographic differentiation of the Amazonian tortoise species Chelonoidis carbonaria and C. denticulata. While C. carbonaria is generally restricted to savannah habitats and adjacent forests, C. denticulata is associated with wet tropical and subtropical forests. Our study suggests a correlation between distinct habitat preferences and phylogeography of the two species. In Maximum Parsimony, Maximum Likelihood and Bayesian analyses, haplotypes of C. carbonaria cluster in several distinct clades reflecting the species’ patchy distribution in savannah habitats. By contrast, haplotypes of C. denticulata are only weakly differentiated; a finding also confirmed by parsimony network analysis. This suggests that the contiguous Amazonian rainforest allows gene flow between populations of the forest-dwelling C. denticulata throughout the range, but significantly impedes gene flow in C. carbonaria. The phylogeographic structure and extant distribution pattern of C. carbonaria is supportive of former Amazonian rainforest fragmentation, enabling the dispersal of savannah species. Based on fossil calibration, we dated divergence times for the C. carbonaria clades using a relaxed molecular clock, resulting in average estimates ranging from 4.0–2.2 mya. This implies that the onset of rainforest fragmentation could predate the Pleistocene considerably. Furthermore, our findings call for further research on geographic and taxonomic variation in C. carbonaria and for a reassessment of the conservation status of the distinct genetic units.

Keywords

Phylogeography Cytochrome b gene Testudinidae Rainforest Forest refugia hypothesis 

Notes

Acknowledgements

The work of Mario Vargas-Ramírez was made possible by a fellowship of the DAAD (Deutscher Akademischer Austausch Dienst). María Cristina Ardíla-Robayo and Willington Ortiz (Instituto de Biología Tropical Roberto Franco, Universidad Nacional de Colombia), Olga Castaño-Mora and Orlando Rangel-Ch. (Instituto de Ciencias Naturales, Universidad Nacional de Colombia), Wilson Pérez (CORPOCESAR, Colombia) and Juan Nicolas Vargas-Gonzales were of invaluable help in Colombia. Marcelo Sánchez-Villagra (Paleontological Institute, University of Zürich) provided information about the fossil record of Chelonoidis. Anke Müller, Anna Hundsdörfer and Christian Kehlmaier (Museum of Zoology Dresden) much assisted Mario Vargas-Ramírez during lab work.

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Copyright information

© Gesellschaft fuer Biologische Systematik 2010

Authors and Affiliations

  • Mario Vargas-Ramírez
    • 1
  • Jérôme Maran
    • 2
  • Uwe Fritz
    • 1
    Email author
  1. 1.Museum of Zoology (Museum für Tierkunde), Senckenberg Natural History Collections DresdenDresdenGermany
  2. 2.L’Association du Refuge des Tortues, Mairie des BessièresBessièresFrance

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