Journal of Physiology and Biochemistry

, Volume 74, Issue 4, pp 539–547 | Cite as

Sceletium tortuosum may delay chronic disease progression via alkaloid-dependent antioxidant or anti-inflammatory action

  • A.C. Bennett
  • A. Van Camp
  • V. López
  • C. SmithEmail author
Original Article


The link between obesity-induced systemic inflammation and decreased insulin signalling is well-known. It is also known that peripherally produced inflammatory cytokines can cross the blood-brain barrier, resulting in the release of neurotoxins that can ultimately lead to the demise of central nervous system integrity. A high-mesembrine Sceletium tortuosum extract was recently shown to possess cytoprotective and mild anti-inflammatory properties in monocytes and to target specific p450 enzymes to reduce adrenal glucocorticoid synthesis. This is significant since the aetiology of both obesity and diabetes is linked to inflammation and excess glucocorticoid production. Given the interlinked nature of glucocorticoid action and inflammation, central immunomodulatory effects of two Sceletium tortuosum extracts prepared by different extraction methods were investigated. Human astrocytes were pre-treated for 30 min, before exposure to Escherichia coli lipopolysaccharide for 23.5 h (in the presence of treatment). Cytotoxicity, mitotoxicity and cytokine responses (basally and in response to inflammatory stimulus) were assessed. In addition, total polyphenol content, antioxidant capacity and selected neural enzyme inhibition capacity were assessed for both extracts. The high-mesembrine Sceletium extract exerted cytoprotective and anti-inflammatory effects. In contrast, the high delta7-mesembrenone extract, rich in polyphenols, exhibited potent antioxidant effect, although with relatively higher risk of adverse effects with overdose. We conclude that both Sceletium tortuosum extracts may be employed as either a preventative supplement or complimentary treatment in the context of obesity and diabetes; however, current data also highlights the impact that extraction methods can have on plant product mechanism of action.


Diabetes Neuroinflammation Oxidative stress Astrocytes Inflammation Type 3 diabetes 



Analysis of variance




Blood-brain barrier


Central nervous system


Dulbecco’s modified eagle’s medium


Dulbecco’s phosphate-buffered saline




Foetal bovine serum


Hank’s balanced salt solution


High-performance liquid chromatography








Monoamine oxidase A


Monocyte chemotactic protein-1


Phosphate-buffered saline


Propidium iodide


Reactive oxygen species


Revolutions per minute


Standard error of the mean


Type 2 diabetes


Type 3 diabetes



We would like to acknowledge the South African National Research Foundation (NRF) for the funding, and Verve Dynamics for the preparation and kind donation of the extracts used in this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© University of Navarra 2018

Authors and Affiliations

  1. 1.Department of Physiological Sciences, Science FacultyStellenbosch UniversityStellenboschSouth Africa
  2. 2.Department of Pharmacy, Faculty of Health SciencesSan Jorge UniversityVillanueva de GállegoSpain

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