Effects of pterostilbene in brown adipose tissue from obese rats
- 449 Downloads
In recent years, much attention has been paid by the scientific community to phenolic compounds as active biomolecules naturally present in foods. Pterostilbene is a resveratrol dimethylether derivative which shows higher bioavailability. The aim of the present study was to analyze the effect of pterostilbene on brown adipose tissue thermogenic markers in a model of genetic obesity, which shows reduced thermogenesis. The experiment was conducted with 30 Zucker (fa/fa) rats that were distributed in three experimental groups: control and two groups orally administered with pterostilbene at 15 and 30 mg/kg body weight/day for 6 weeks. Gene expression of uncoupling protein 1 (Ucp1), peroxisome proliferator-activated receptor γ co-activator 1 α (Pgc-1α), carnitine palmitoyl transferase 1b (Cpt1b), peroxisome proliferator-activated receptor α (Pparα), nuclear respiratory factor 1 (Nfr1), and cyclooxygenase-2 (Cox-2); protein expression of PPARα, PGC-1α, p38 mitogen-activated protein kinase (p38 MAPK), UCP1 and glucose transporter (GLUT4); and enzyme activity of CPT 1b and citrate synthase (CS) were assessed in interscapular brown adipose tissue. With the exception of Pgc-1α expression, all these parameters were significantly increased by pterostilbene administration. These results show for the first time that pterostilbene increases thermogenic and oxidative capacity of brown adipose tissue in obese rats. Whether these effects effectively contribute to the antiobesity properties of these compound needs further research.
KeywordsPterostilbene Brown adipose tissue Obese rats Fatty acid oxidation GLUT4
This study was supported by grants from the Ministerio de Economía y Competitividad (AGL-2015-65719-R), Fondo Europeo de Desarrollo Regional (FEDER), Instituto de Salud Carlos III (CIBERobn), Government of the Basque Country (IT-572-13), and University of the Basque Country (UPV/EHU) (ELDUNANOTEK UFI11/32). The authors would also like to acknowledge the financial support of Biodonostia Institute. I. Milton-Laskibar is a predoctoral fellows from the Government of the Basque Country.
Compliance with ethical standards
The experiment was conducted with thirty 5-week-old male Zucker (fa/fa) rats purchased from Charles Rivers Laboratories (Barcelona, Spain) and was conducted in accordance with the institution’s guide for the care and use of laboratory animals (approval document reference CEBA CEEA14/018).
- 7.Arias N, Miranda J, Macarulla MT, Aguirre L, Fernández-Quintela A, Andres-Lacueva C, Urpi-Sarda M, Portillo MP (2014) The combination of resveratrol and conjugated linoleic acid attenuates the individual effects of these molecules on triacylglycerol metabolism in adipose tissue. Eur J Nutr 53:575–582CrossRefPubMedGoogle Scholar
- 21.Kava R, Greenwood M, PR J (1990) New rat models of obesity and type II diabetes: Zucker (fa/fa) rat. In, ILAR, p 4–88Google Scholar
- 23.Livak K, Schmittgen T Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)). Method 25:402–408Google Scholar
- 26.Marette A, Tulp O, Buckowiecki L (1990) Defective brown adipose tissue thermogenesis in obesity. Role insulin resistance and diabetes. John Libbey and Company Ltd, LondonGoogle Scholar
- 32.Paul S, DeCastro AJ, Lee HJ, Smolarek AK, So JY, Simi B, Wang CX, Zhou R, Rimando AM, Suh N (2010) Dietary intake of pterostilbene, a constituent of blueberries, inhibits the beta-catenin/p65 downstream signaling pathway and colon carcinogenesis in rats. Carcinogenesis 31:1272–1278CrossRefPubMedPubMedCentralGoogle Scholar
- 38.Srere P (1969) Citrate synthase. In, Methods Enzymology, p 3–11Google Scholar