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Journal of Physiology and Biochemistry

, Volume 72, Issue 4, pp 657–667 | Cite as

ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner

  • Xing Liu
  • Kun Ren
  • Rong Suo
  • Sheng-Lin Xiong
  • Qing-Hai Zhang
  • Zhong-Cheng Mo
  • Zhen-Li Tang
  • Yue Jiang
  • Xiao-Shan Peng
  • Guang-Hui YiEmail author
Original Paper

Abstract

Sphingosine-1-phosphate (S1P), which has emerged as a pivotal signaling mediator that participates in the regulation of multiple cellular processes, is derived from various cells, including vascular endothelial cells. S1P accumulates in lipoproteins, especially HDL, and the majority of free plasma S1P is bound to HDL. We hypothesized that HDL-associated S1P is released through mechanisms associated with the HDL maturation process. ApoA-I, a major HDL apolipoprotein, is a critical factor for nascent HDL formation and lipid trafficking via ABCA1. Moreover, apoA-I is capable of promoting bidirectional lipid movement through SR-BI. In the present study, we confirmed that apoA-I can facilitate the production and release of S1P by HUVECs. Furthermore, we demonstrated that ERK1/2 and SphK activation induced by apoA-I is involved in the release of S1P from HUVECs. Inhibitor and siRNA experiments showed that ABCA1 and SR-BI are required for S1P release and ERK1/2 phosphorylation induced by apoA-I. However, the effects triggered by apoA-I were not suppressed by inhibiting ABCA1/JAK2 or the SR-BI/Src pathway. S1P released due to apoA-I activation can stimulate the (ERK1/2)/SphK1 pathway through S1PR (S1P receptor) 1/3. These results indicated that apoA-I not only promotes S1P release through ABCA1 and SR-BI but also indirectly activates the (ERK1/2)/SphK1 pathway by releasing S1P to trigger their receptors. In conclusion, we suggest that release of S1P induced by apoA-I from endothelial cells through ABCA1 and SR-BI is a self-positive-feedback process: apoA-I-(ABCA1 and SR-BI)-(S1P release)-S1PR-ERK1/2-SphK1-(S1P production)-(more S1P release induced by apoA-I).

Keywords

S1P SR-BI ABCA1 SphK S1P receptors Release 

Abbreviations

ABC

ATP-binding cassette

ERK1/2

Extracellular receptor kinase 1/2

FC

Free cholesterol

HDAC

Histone deacetylases

HDL

High-density lipoprotein

HDL-S1P

HDL-associated S1P

HPLC

High-performance liquid chromatography

HUVECs

Human umbilical vein endothelial cells

JAK2

Janus kinase 2

PL

Phospholipid

S1P

Sphingosine-1-phosphate

S1PR

S1P receptor

SphK

Sphingosine kinase

SR-BI

Scavenger receptor class B type I

TRAF2

TNF receptor-associated factor 2

Notes

Acknowledgments

This work was sponsored by the National Natural Science Foundation of China (Nos. 81270360 and 31400652), Scientific Research Fund of Hunan Provincial Education Department (13C838), the Science and Technology Planning Project of Hunan Provincial Science and Technology Department (2014FJ2012), the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions (2008-244) of Hunan Province, and the construct program of the key discipline in Hunan Province, China (Basic Medicine Sciences in University of South China, Xiangjiaofa No. [2011]76).

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Copyright information

© University of Navarra 2016

Authors and Affiliations

  • Xing Liu
    • 1
  • Kun Ren
    • 1
  • Rong Suo
    • 1
  • Sheng-Lin Xiong
    • 2
  • Qing-Hai Zhang
    • 3
  • Zhong-Cheng Mo
    • 4
  • Zhen-Li Tang
    • 1
  • Yue Jiang
    • 1
  • Xiao-Shan Peng
    • 1
  • Guang-Hui Yi
    • 1
    Email author
  1. 1.Institute of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan ProvinceUniversity of South ChinaHengyang CityChina
  2. 2.You Country People’s HospitalZhuzhouChina
  3. 3.Clinical Research Institution, The First Affiliated HospitalUniversity of South ChinaHengyangChina
  4. 4.Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, Life Science Research CenterUniversity of South ChinaHengyangChina

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