Journal of Physiology and Biochemistry

, Volume 71, Issue 4, pp 763–772 | Cite as

KPNβ1 promotes palmitate-induced insulin resistance via NF-κB signaling in hepatocytes

  • Suxin Wang
  • Yun Zhao
  • Nana Xia
  • Wanlu Zhang
  • Zhuqi Tang
  • Cuifang Wang
  • Xiaohui Zhu
  • Shiwei Cui
Original Paper


It has been intensively studied that inflammation contributes to the insulin resistance development in obesity-induced type 2 diabetes mellitus (T2DM). In this study, we assessed the effect of karyopherin β1 (KPNβ1) in hepatic insulin resistance and the underlying mechanisms using high-fat diet (HFD) fed mice and palmitate (PA)-stimulated hepatocytes (HepG2). KPNβ1 expression is increased in the HFD fed mice liver. PA upregulated KPNβ1 expression in HepG2 cells in a time-dependent manner. PA also increased pro-inflammatory cytokines expression, including tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β). KPNβ1 knockdown reversed PA-induced pro-inflammatory cytokines expression and insulin-stimulated glucose uptake in HepG2 cells. In addition, KPNβ1 knockdown reduced intracellular lipid accumulation. Mechanistically, KPNβ1 transports nuclear factor kB (NF-κB) p65 from the cytoplasm to the nucleus to increase pro-inflammatory genes expression. In summary, KPNβ1 acts as a positive regulator in the NF-κB pathway to enhance palmitate-induced inflammation response and insulin resistance in HepG2 cells.


KPNβ1 Inflammation Insulin resistance NF-κB 


Compliance with ethical standards

All procedures involving animals were approved by the Experimental Animal Center of Nantong University.

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© University of Navarra 2015

Authors and Affiliations

  • Suxin Wang
    • 1
  • Yun Zhao
    • 1
  • Nana Xia
    • 1
  • Wanlu Zhang
    • 1
  • Zhuqi Tang
    • 1
  • Cuifang Wang
    • 1
  • Xiaohui Zhu
    • 1
  • Shiwei Cui
    • 1
  1. 1.Department of Endocrinology and Metabolic DiseasesAffiliated Hospital of Nantong UniversityNantongChina

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