Journal of Physiology and Biochemistry

, Volume 71, Issue 4, pp 743–751 | Cite as

Protective effect of rosmarinic acid against oxidative stress biomarkers in liver and kidney of strepotozotocin-induced diabetic rats

  • Nadia Mushtaq
  • Roberta Schmatz
  • Mushtaq Ahmed
  • Luciane Belmonte Pereira
  • Pauline da Costa
  • Karine Paula Reichert
  • Diéssica Dalenogare
  • Luana Paula Pelinson
  • Juliano Marchi Vieira
  • Naiara Stefanello
  • Lizielle Souza de Oliveira
  • Nadia Mulinacci
  • Maria Bellumori
  • Vera Maria Morsch
  • Maria Rosa Schetinger
Original Paper


In the present study, we investigated the efficiency of rosmarinic acid (RA) in preventing the alteration of oxidative parameters in the liver and kidney of diabetic rats induced by streptozotocin (STZ). The animals were divided into six groups (n = 8): control, ethanol, RA 10 mg/kg, diabetic, diabetic/ethanol, and diabetic/RA 10 mg/kg. After 3 weeks of treatment, we found that TBARS levels in liver and kidney were significantly increased in the diabetic/saline group and the administration of RA prevented this increase in the liver and kidney (P < 0.05). Diabetes caused a significant decrease in the activity of superoxide dismutase (SOD) and catalase (CAT) in the diabetes/saline group (P < 0.05). However, the treatment with 10 mg/kg RA (antioxidant) prevented this alteration in SOD and CAT activity in the diabetic RA group (P < 0.05). In addition, RA reverses the decrease in ascorbic acid and non-protein-thiol (NPSH) levels in diabetic rats. The treatment with RA also prevented the decrease in the Delta-aminolevulinic acid dehydratase (ALA-D) activity in the liver and kidney of diabetic rats. Furthermore, RA did not have any effect on glycemic levels. These results indicate that RA effectively reduced the oxidative stress induced by STZ, suggesting that RA is a potential candidate for the prevention and treatment of pathological conditions in diabetic models.


Rosmarinic acid Oxidative stress Strepotozotocin 



We wish to thank the Academy of Sciences for the Developing World (TWAS) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support.


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Copyright information

© University of Navarra 2015

Authors and Affiliations

  • Nadia Mushtaq
    • 1
  • Roberta Schmatz
    • 1
    • 4
  • Mushtaq Ahmed
    • 2
  • Luciane Belmonte Pereira
    • 1
  • Pauline da Costa
    • 1
  • Karine Paula Reichert
    • 1
  • Diéssica Dalenogare
    • 1
  • Luana Paula Pelinson
    • 1
  • Juliano Marchi Vieira
    • 1
  • Naiara Stefanello
    • 1
  • Lizielle Souza de Oliveira
    • 1
  • Nadia Mulinacci
    • 3
  • Maria Bellumori
    • 3
  • Vera Maria Morsch
    • 1
  • Maria Rosa Schetinger
    • 1
  1. 1.Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UniversitárioSanta MariaBrazil
  2. 2.Department of BiotechnologyUniversity of Science and TechnologyBannuPakistan
  3. 3.Department of NEUROFARBAUniversity of FlorenceSesto (Firenze)Italy
  4. 4.Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, IFRS-Câmpus IbirubáIbirubáBrazil

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