Neuroprotective effect of schizandrin A on oxygen and glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons
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Brain ischemia appears to be associated with innate immunity. Recent reports showed that C3a and C5a, as potent targets, might protect against ischemia induced cell death. In traditional Chinese medicine, the fruit of Schizandra chinesis Baill (Fructus schizandrae) has been widely used as a tonic. In the present study, we sought to evaluate the neuroprotective effects of schizandrin A, a composition of S. chinesis Baill, against oxygen and glucose deprivation followed by reperfusion (OGD/R)-induced cell death in primary culture of rat cortical neurons, and to test whether C3a and C5a affected cortical neuron recovery from ischemic injury after schizandrin A treatment. The results showed that schizandrin A significantly reduced cell apoptosis and necrosis, increased cell survival, and decreased intracellular calcium concentration ([Ca2+]i) and lactate dehydrogenase (LDH) release in primary culture of rat cortical neurons after OGD/R. Mechanism studies suggested that the modulation of extracellular-regulated kinase (ERK), c-Jun NH2-terminal kinases (JNK), and p38, as well as caspase-3 activity played an important role on the progress of neuronal apoptosis. C5aR participated in the neuroprotective effect of schizandrin A in primary culture of rat cortical neurons after OGD/R. Our findings suggested that schizandrin A might act as a candidate therapeutic target drug used for brain ischemia and related diseases.
KeywordsSchizandrin A Apoptosis Ischemia Mitogen-activated protein kinase (MAPK) C5aR
c-Jun NH2-terminal kinases
Dulbecco’s Modified Eagle’s Medium
Fetal bovine serum
Intracellular calcium concentrations
Luciferase reporter construct has three copies of CRE in the 5′ region
Mitogen-activated protein kinase
Oxygen and glucose deprivation followed by reperfusion
Polymerase chain reaction
The third complement component
The fifth complement component
This study was supported by funding for scientific research of Nantong University (Program No. 03080374, 03080364, 03041236, and 03080471), research funding funded by Provincial Key Lab of Jiangsu Province of China (Program No. 05012066), and research funding funded by Natural Science Research Program of Jiangsu Education Department (Program No.13KJB310014).
Conflict of interests
The authors declare no competing interests.
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