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Exercise-induced changes of MCT1 in cardiac and skeletal muscles of diabetic rats induced by high-fat diet and STZ

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Abstract

We hypothesized that a part of therapeutic effects of endurance training on insulin resistance is mediated by increase in cardiac and skeletal muscle mitochondrial lactate transporter, monocarboxylate transporter 1 (MCT1). Therefore, we examined the effect of 7 weeks endurance training on the mRNA and protein expression of MCT1 and MCT4 and their chaperon, CD147, on both sarcolemmal and mitochondrial membrane, separately, in healthy and type 2 diabetic rats. Diabetes was induced by injection of low dose of streptozotocin and feeding with high-fat diet. Insulin resistance was confirmed by homeostasis model assessment-estimated insulin resistance index and accuracy of two membranes separation was confirmed by negative control markers (glucose transporter 1 and cytochrome c oxidase. Real-time PCR and western blotting were used for mRNA and protein expression, respectively. Diabetes dramatically reduced MCT1 and MCT4 mRNA and their expression on sarcolemmal membrane whereas the reduction in MCT1 expression was less in mitochondrial membrane. Training increased the MCT1 mRNA and protein expression in both membranes and decreased insulin resistance as an adaptive consequence. In both tissues increase in CD147 mRNA was only parallel to MCT1 expression. The response of MCT1 on sarcolemmal and mitochondrial membranes was different between cardiac and skeletal muscles which indicate that intracellular lactate kinetic is tissue specific that allows a tissue to coordinate whole organism metabolism.

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Acknowledgments

This study was supported by a Centre grant from the Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences. We also acknowledge all our collaborators.

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Authors and Affiliations

  1. Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Shahid Bahonar University of Kerman, Kerman, Iran

    Rohollah Nikooie & Malihe Aveseh

  2. Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Kharazmi University, Tehran, Iran

    Hamid Rajabi

  3. Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Tarbiat Modares University, Tehran, Iran

    Reza Gharakhanlu

  4. Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Fereshteh Atabi

  5. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular, Sciences Institute, Tehran University of Medical Sciences, P.O. Box 14395/1179, Tehran, Iran

    Kobra Omidfar

  6. Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran

    Bagher Larijani

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  1. Rohollah Nikooie
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Correspondence to Kobra Omidfar.

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Nikooie, R., Rajabi, H., Gharakhanlu, R. et al. Exercise-induced changes of MCT1 in cardiac and skeletal muscles of diabetic rats induced by high-fat diet and STZ. J Physiol Biochem 69, 865–877 (2013). https://doi.org/10.1007/s13105-013-0263-6

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  • DOI: https://doi.org/10.1007/s13105-013-0263-6

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