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Journal of Physiology and Biochemistry

, Volume 69, Issue 3, pp 595–600 | Cite as

Lipoic acid inhibits adiponectin production in 3T3-L1 adipocytes

  • Pedro L. Prieto-Hontoria
  • Marta Fernández-Galilea
  • Patricia Pérez-Matute
  • J. Alfredo Martínez
  • María J. Moreno-Aliaga
Original Paper

Abstract

Lipoic acid (LA) is a naturally occurring compound with antioxidant properties. Recent attention has been focused on the potential beneficial effects of LA on obesity and related metabolic disorders. Dietary supplementation with LA prevents insulin resistance and upregulates adiponectin, an insulin-sensitizing adipokine, in obese rodents. The aim of this study was to investigate the direct effects of LA on adiponectin production in cultured adipocytes, as well as the potential signaling pathways involved. For this purpose, fully differentiated 3T3-L1 adipocytes were treated with LA (1–500 μM) during 24 h. The amount of adiponectin secreted to media was detected by ELISA, while adiponectin mRNA expression was determined by RT-PCR. Treatment with LA induced a dose-dependent inhibition on adiponectin gene expression and protein secretion. Pretreatment with the PI3K inhibitor LY294002 inhibited adiponectin secretion and mRNA levels, and significantly potentiated the inhibitory effect of LA on adiponectin secretion. The AMPK activator AICAR also reduced adiponectin production, but surprisingly, it was able to reverse the LA-induced inhibition of adiponectin. The JNK inhibitor SP600125 and the MAPK inhibitor PD98059 did not modify the inhibitory effect of LA on adiponectin. In conclusion, our results revealed that LA reduces adiponectin secretion in 3T3-L1 adipocytes, which contrasts with the stimulation of adiponectin described after in vivo supplementation with LA, suggesting that an indirect mechanism or some in vivo metabolic processing is involved.

Keywords

Lipoic acid Obesity Adiponectin Adipocytes 

Notes

Acknowledgments

This work has been supported by the Ministry of Science and Innovation of the Government of Spain (AGL 2009-10873/ALI and AGL 2006-04716/ALI) and by Línea Especial: “Nutrición, Obesidad y Salud” (University of Navarra). PL Prieto-Hontoria was supported by a research grant by Danone Institute, Spain. CTP, CIBERobn, and RETICS Network are gratefully acknowledged.

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© University of Navarra 2013

Authors and Affiliations

  • Pedro L. Prieto-Hontoria
    • 1
  • Marta Fernández-Galilea
    • 1
  • Patricia Pérez-Matute
    • 1
    • 2
  • J. Alfredo Martínez
    • 1
  • María J. Moreno-Aliaga
    • 1
  1. 1.Department of Nutrition, Food Science and PhysiologyUniversity of NavarraPamplonaSpain
  2. 2.HIV and Associated Metabolic Alterations Unit, Infectious Diseases AreaCenter for Biomedical Research of La Rioja (CIBIR)LogroñoSpain

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