Journal of Physiology and Biochemistry

, Volume 69, Issue 2, pp 313–323 | Cite as

Antihypercholesterolemic and antioxidative effects of an extract of the oyster mushroom, Pleurotus ostreatus, and its major constituent, chrysin, in Triton WR-1339-induced hypercholesterolemic rats

  • Ramalingam Anandhi
  • Thangaraj Annadurai
  • Thirugnanasambandhar S. Anitha
  • Arumugam R. Muralidharan
  • Kalifulla Najmunnisha
  • Vasanthi Nachiappan
  • Philip A. Thomas
  • Pitchairaj Geraldine
Original Paper


Hypercholesterolemia and oxidative stress are known to accelerate coronary artery disease and progression of atherosclerotic lesions. In the present study, an attempt was made to evaluate the putative antihypercholesterolemic and antioxidative effects of an ethanolic extract of the oyster mushroom (Pleurotus ostreatus) and chrysin, one of its major components, in hypercholesterolemic rats. Hypercholesterolemia was induced in rats by a single intraperitoneal injection of Triton WR-1339 (300 mg/kg body weight (b.wt.)), which resulted in persistently elevated blood/serum levels of glucose, lipid profile parameters (total cholesterol, triglycerides, low-density lipoprotein-, and very low-density lipoprotein-cholesterol), and of hepatic marker enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase). In addition, lowered mean activities of hepatic antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase) and lowered mean levels of nonenzymatic antioxidants (reduced glutathione, vitamin C, and vitamin E) were observed. Oral administration of the mushroom extract (500 mg/kg b.wt.) and chrysin (200 mg/kg b.wt.) to hypercholesterolemic rats for 7 days resulted in a significant decrease in mean blood/serum levels of glucose, lipid profile parameters, and hepatic marker enzymes and a concomitant increase in enzymatic and nonenzymatic antioxidant parameters. The hypercholesterolemia-ameliorating effect was more pronounced in chrysin-treated rats than in extract-treated rats, being almost as effective as that of the standard lipid-lowering drug, lovastatin (10 mg/kg b.wt.). These results suggest that chrysin, a major component of the oyster mushroom extract, may protect against the hypercholesterolemia and elevated serum hepatic marker enzyme levels induced in rats injected with Triton WR-1339.


Hypercholesterolemia Triton WR-1339 Oxidative stress Chrysin Oyster mushroom 



The instrumentation facility provided by the University Grant Commission-Special Assistance Programme of the Department of Animal Science, Bharathidasan University, is acknowledged. Financial assistance provided by the Department of Science and Technology-Promotion of University Research and Scientific Excellence to the first author is gratefully acknowledged.

Conflict of interest

The authors declare that there are no conflicts of interest.


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Copyright information

© University of Navarra 2012

Authors and Affiliations

  • Ramalingam Anandhi
    • 1
  • Thangaraj Annadurai
    • 1
  • Thirugnanasambandhar S. Anitha
    • 1
  • Arumugam R. Muralidharan
    • 1
  • Kalifulla Najmunnisha
    • 1
  • Vasanthi Nachiappan
    • 3
  • Philip A. Thomas
    • 2
  • Pitchairaj Geraldine
    • 1
  1. 1.Department of Animal Science, School of Life SciencesBharathidasan UniversityTiruchirappalliIndia
  2. 2.Institute of OphthalmologyJoseph Eye HospitalTiruchirappalliIndia
  3. 3.Department of Biochemistry, School of Life SciencesBharathidasan UniversityTiruchirappalliIndia

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