Journal of Physiology and Biochemistry

, Volume 66, Issue 4, pp 321–327

Glutathione system in young spontaneously hypertensive rats

  • S. K. Lee
  • Sundaram Arunkumar
  • K. N. S. Sirajudeen
  • H. J. Singh
Original Paper

Abstract

Glutathione (GSH) forms a part of the antioxidant system that plays a vital role in preventing oxidative stress, and an imbalance in the oxidant/antioxidant system has been linked to the pathogenesis of hypertension. The aim of this study was to investigate the status of the GSH system in the kidney of spontaneously hypertensive rats (SHR). Components of the GSH system, including glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST), and total GSH content, were measured in the kidneys of 4, 6, 8, 12, and 16 weeks old SHR and Wistar–Kyoto (WKY) rats. Systolic blood pressure of SHR was significantly higher from the age of 6 weeks onwards compared with age-matched WKY rats. GPx activity in the SHR was significantly lower from the age of 8 weeks onwards when compared to that in age-matched WKY rats. No significant differences were evident in the GPx-1 protein abundance, and its relative mRNA levels, GR, GST activity, and total GSH content between SHR and age-matched WKY rats. The lower GPx activity suggests of an impairment of the GSH system in the SHR, which might be due to an abnormality in its protein rather than non-availability of a cofactor. Its role in the development of hypertension in SHR however remains unclear.

Keywords

Glutathione Glutathione peroxidase Hypertension SHR 

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Copyright information

© University of Navarra 2010

Authors and Affiliations

  • S. K. Lee
    • 1
  • Sundaram Arunkumar
    • 1
  • K. N. S. Sirajudeen
    • 2
  • H. J. Singh
    • 3
  1. 1.Department of Physiology, School of Medical SciencesUniversiti Sains MalaysiaKubang KerianMalaysia
  2. 2.Department of Chemical Pathology, School of Medical SciencesUniversiti Sains MalaysiaKubang KerianMalaysia
  3. 3.Faculty of MedicineLevel 20 S & T Building, Universiti Teknologi MARAShah AlamMalaysia

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