Translational Stroke Research

, Volume 2, Issue 1, pp 42–50 | Cite as

Manganese Superoxide Dismutase Deficiency Exacerbates Ischemic Brain Damage Under Hyperglycemic Conditions by Altering Autophagy

  • Suresh L. Mehta
  • Yanling Lin
  • Wenge Chen
  • Fengshan Yu
  • Luyi Cao
  • Qingping He
  • Pak H. Chan
  • P. Andy Li


Both preischemic hyperglycemia and suppression of SOD2 activity aggravate ischemic brain damage. This study was undertaken to assess the effect of SOD2 mutation on ischemic brain damage and its relation to the factors involved in autophagy regulation in hyperglycemic wild-type (WT) and heterozygous SOD2 knockout (SOD2–/+) mice subjected to 30-min transient focal ischemia. The brain samples were analyzed at 5 and 24 h after recirculation for ischemic lesion volume, superoxide production, and oxidative DNA damage and protein levels of Beclin 1, damage-regulated autophagy modulator (DRAM), and microtubule-associated protein 1 light chain 3 (LC3). The results revealed a significant increase in infarct volume in hyperglycemic SOD2–/+ mice, and this was accompanied with an early (5 h) significant rise in superoxide production and reduced SOD2 activity in SOD2–/+ mice as compared to WT mice. The superoxide production is associated with oxidative DNA damage as indicated by colocalization of the dihydroethidium (DHE) signal with 8-OHdG fluorescence in SOD2–/+ mice. In addition, while ischemia in WT hyperglycemics increased the levels of autophagy markers Beclin 1, DRAM, and LC3, ischemia in hyperglycemic, SOD2-deficient mice suppressed the levels of autophagy stimulators. These results suggest that SOD2 knockdown exacerbates ischemic brain damage under hyperglycemic conditions via increased oxidative stress and DNA oxidation. Such effect is associated with suppression of autophagy regulators.


Cerebral ischemia SOD2 Hyperglycemia Oxidative stress Autophagy 



This work was supported by NIH grant 7R01DK075476. The BRITE is partially funded by the Golden Leaf Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Suresh L. Mehta
    • 1
  • Yanling Lin
    • 2
  • Wenge Chen
    • 1
    • 3
  • Fengshan Yu
    • 4
  • Luyi Cao
    • 1
  • Qingping He
    • 1
  • Pak H. Chan
    • 4
  • P. Andy Li
    • 1
  1. 1.Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise (BRITE)North Carolina Central UniversityDurhamUSA
  2. 2.Reproductive Biogenesis InstituteUniversity of Hawaii School of MedicineHonoluluUSA
  3. 3.Department of Laser Therapeutics, Affiliated Hospital, College of Clinical SciencesNingxia Medical UniversityNingxiaPeople’s Republic of China
  4. 4.Department of NeurosurgeryStanford University School of MedicineStanfordUSA

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