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Journal of Crop Science and Biotechnology

, Volume 21, Issue 4, pp 335–341 | Cite as

Identification and Confirmation of New Molecular Markers Linked to The Fertility Restorer Genes in Sugar Beet

  • Peyman NorouziEmail author
  • Abazar Rajabi
Research Article
  • 2 Downloads

Abstract

It is of special importance to track the fertility restoring alleles in plants such as sugar beet in which cytoplasmic male sterility (CMS) is used to produce hybrid seed, so that the possibility of detection of presence and absence of specific restoring alleles facilitates to a great extent the development of new O-type lines. For tagging of fertility restorer genes in sugar beet, an F2 population was used. At first, fertility scoring of F2 single plants in the field was done and the population was divided to four groups including fertile, half fertile, yellow sterile, and white sterile. Then, leaf sampling from the F2 single plants was accomplished for DNA extraction. Polymorphism of random primers of RAPD and STS primers was investigated in fertile and sterile bulks by bulk segregant analysis method. Several RAPD and STS primers produced polymorphism in fertile and sterile bulks. After testing the selected primers on the DNA of single plants of the bulk, F2 population and other sugar beet genotypes, one STS repulsion marker and one coupling marker were identified to distances of 17 and 8% recombination frequencies, respectively. In this research, the molecular basis of the genomic regions of fertility restoring loci of sugar beet was studied for the first time in Iran. These markers could be used for preliminary selection of O-type plants from pollinator populations and hence the time and costs related to pair crosses for identification of O-type character could be saved.

Key words

Beta vulgaris BSA method male sterility O-type STS marker 

Abbreviations

BSA

bulk segregant analysis

CMS

cytoplasmic male sterility

PCR

polymerase chain reaction

QTL

quantitative traits loci

RAPD

random amplified polymorphic DNA

RFLP

restriction fragment length polymorphism

STS

sequence tagged site

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Copyright information

© Korean Society of Crop Science (KSCS) and Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Associate professor of Sugar Beet Seed Institute, Agricultural ResearchEducation and Extension Organization (AREEO)KarajIran

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