Journal of Crop Science and Biotechnology

, Volume 16, Issue 4, pp 277–283 | Cite as

Morphological, physiological and antioxidant responses of some Iranian grapevine cultivars to methyl jasmonate application

  • Siamak Shirani Bidabadi
  • Hamidreza Mehri
  • Cyrus Ghobadi
  • Bahram Baninasab
  • Marzieh Afazel
Research Article


The role of exogenously applied Methyl jasmonate (MeJA) in morphological responses, photochemical efficiency, changes of malondialdehyde content, and the activities of some antioxidant enzymes were investigated in four Iranian grapevine cultivars. MeJA improved morphological traits containing dry and fresh weight, node number, and shoot length. MeJA induced an oxidative stress, as shown by an increase in lipid peroxidation. Activities of catalase, peroxidase, and ascorbate peroxidase were higher in MeJA-treated grapevines than in controls while the relative water content and leaf water loss of grapevine cultivars demonstrated a non-significant difference between the control and varying levels of MeJA. MeJA was positively affected in recovery of the leaf chlorophyll fluorescence (photochemical efficiency) of grapevine cultivars, although the mean proline content of MeJA-treated grapevines indicated a significant decrease when compared with those of the controls. These results suggest that MeJA could act as an intervener in grapevine responses by the enhancing the activity of antioxidants and recovery of photochemical efficiency, leading to enhanced grapevine performance.

Key words

antioxidant enzymes grapevines melondialdehyde content metyl jasmonate photochemical efficiency 



ascorbate peroxidase




dry weight


fresh weight


leaf water lose




methyl jasmonate




reactive oxygen species


relative water content


thiobarbituric acid


trichloroacetic acid


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alleweldt G, Spiegel-Roy P, Reisch B. 1990. Grapes (Vitis), In JN Moore, JR Ballington, eds, Genetic Resources of Temperate Fruit and Nut Crops, Acta Hortic. 290: 291–337Google Scholar
  2. Bates LS, Waldern RP, Teare ID. 1973. Rapid determination of free proline from water stress studies. Plant Soil 39: 205–207CrossRefGoogle Scholar
  3. Bhardway R, Singhal G. 1981. Effect of water stress on photochemical activity of chloroplast during greening etiolated barley seedlings. Plant Cell Physiol. 22: 155–162Google Scholar
  4. Cheong JJ, Choi YD. 2003. Methyl jasmonate as a vital substance in plants. Trends Genet. 19: 409–413PubMedCrossRefGoogle Scholar
  5. Chong TM, Abdullah MA, Fadzillah NM, Lai OM, Lajis NH. 2005. Jasmonic acid elicitation of anthraquinones with some associated. Enzyme Microb. Technol. 36: 469–477CrossRefGoogle Scholar
  6. Ding CK, Wang CY, Gross KC, Smith DL. 2002. Jasmonate and salicylate induce the expression of athogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta 214: 895–901PubMedCrossRefGoogle Scholar
  7. Fang WC, Kao CH. 2001. Inhibition of methyl jasmonatepromoted senescence in rice leaves by a metal chelator, 2, 2′-bipyridine. Plant Growth Regul. 33: 87–93CrossRefGoogle Scholar
  8. FAO. 1997. FAO production year book for 1997. 51, FAO, RomeGoogle Scholar
  9. Fatahi R, Ebadi A, Bassil N, Mehlenbacher SA, Zamani Z. 2003. Characterization of Iranian grapevine cultivars using microsatellite markers. Vitis 42: 185–192Google Scholar
  10. Huguet RV, Sulpice R, Lefort C, Maerskalck V, Emery N, Larher FR. 2003. The suppression of osmoinduced proline response of Brassica napus L. var oleifera discs by polyunsaturated fatty acids and methyl-jasmonate. Plant Sci. 164: 119–127CrossRefGoogle Scholar
  11. Hung KT, Kao CH. 1998. Involvement of lipid peroxidation in methyl senescence in detached rice leaves. Plant Growth Regul. 24: 17–21CrossRefGoogle Scholar
  12. Hung KT, Kao CH. 2007. The participation of hydrogen peroxide in methyl jasmonate-induced NH4+ accumulation in rice leaves. J. Plant Physiol. 164: 1469–1479PubMedCrossRefGoogle Scholar
  13. Heil M. 2004. Induction of two indirect defenses benefits lima bean (Phaseolus lunatus, Fabaceae) in nature. J Ecol. 92: 527–536CrossRefGoogle Scholar
  14. Jiang MY, Zhang JH. 2002. Water stress-induced abscisic accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J. Exp. Bot. 379: 2401–2410CrossRefGoogle Scholar
  15. Jung S. 2004. Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Plant Physiol. Biochem. 42: 225–231PubMedCrossRefGoogle Scholar
  16. Keramat B, Kalantari KM, Arvin MJ. 2009. Effects of methyl jasmonate in regulating cadmium induced oxidative stress in soybean plant (Glycine max L.). Afr. J. Biotechnol. 3: 240–244Google Scholar
  17. Koda Y. 1997. Possible involvement of jasmonates in various morphogenic events. Physiol. Plant. 100: 639–646CrossRefGoogle Scholar
  18. Mahmood M, Shirani Bidabadi S, Ghobadi S, Gray DJ. 2012. Effect of methyl jasmonate treatments on alleviation of polyethylene glycol-mediated water stress in banana (Musa accuminata cv. Berangan, AAA) shoot tip cultures. Plant Growth Regul. 68: 161–169CrossRefGoogle Scholar
  19. Mata CG, Lamattina L. 2001. Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiol. 126: 1196–1204PubMedCentralCrossRefGoogle Scholar
  20. Meir S, Droby S, Davidson H, Alsevia S, Cohen L, Horev B, Philosoph-Hadas S. 1998. Suppression of Botrytis rot in cut rose flowers by postharvest application of methyl jasmonate. Postharvest Biol. Tech. 13: 235–243CrossRefGoogle Scholar
  21. Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405–410PubMedCrossRefGoogle Scholar
  22. Mohammad BA, Hahn EJ, Peak Y. 2007. Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures. Molecules 12: 607–621CrossRefGoogle Scholar
  23. Nakano Y, Asada YK. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Photochem. Photobiol. 37: 679–690Google Scholar
  24. Nejatian MA. 2012. Collection and evaluation of grapevine genotypes of the Gazvine. FAO, 28 pGoogle Scholar
  25. Norastehnia A, Nojavan AM. 2006. Effect of methyl jasmonate on the enzymatic antioxidant defense system in maize seedling subjected to paraquat. Asian J. Plant Sci. 5: 17–23CrossRefGoogle Scholar
  26. Parthier B. 1991. Jasmonates, new regulators of plant growth and development: many facts and few hypotheses on their actions. Bot. Acta 104: 446–454CrossRefGoogle Scholar
  27. Pospisilova J. 2003. Participation of phytohormones in the stomatal regulation of gas exchange during water stress. Biol. Plant. 46: 491–506CrossRefGoogle Scholar
  28. Ravinkar M, Zel J, Plaper I, Spacapan A. 1993. Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. J. Plant Growth Regul. 12: 73–77CrossRefGoogle Scholar
  29. Ravinkar, M, Rode J, Gogala N, Benedicic D. 1990. Regulation of organogenesis with jasmonic acid. Acta Hort. 280: 169–172Google Scholar
  30. Rohwer CL, Erwin JE. 2008. Horticultural applications of jasmonates: a review. J. Hort. Sci. Biotechnol. 83: 283–304Google Scholar
  31. Santos I, Salema R. 2000. Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regul. 30: 133–138CrossRefGoogle Scholar
  32. Sasaki SY, Taki N, Obayashi T, Aono M, Matsumoto F et al. 2005. Coordinated activation of metabolic pathways for antioxidants and defense compounds by jasmonates and their roles in stress tolerance in Arabidopsis. Plant J. 44: 653–680CrossRefGoogle Scholar
  33. Sharifi P, Amirnia R, Majidi E, Hadi H, Roustaii M, Nakhoda B, Alipoor HM, Moradi F. 2012. Relationship between drought stress and some antioxidant enzymes with cell membrane and chlorophyll stability in wheat lines. Afr. J. Microbiol. Res. 6: 617–623Google Scholar
  34. Srinivas ND, Rashmi KR, Raghavarao KSMS. 1999. Extraction and purification of a plant peroxidase by aqueous two-phase extraction coupled with gel filtration. Process Biochem. 35: 43–48CrossRefGoogle Scholar
  35. Ueda J, Saniewski M. 2006. Methyl jasmonate-induced stimulation of chlorophyll formation in the basal part of tulip bulbs kept under natural light conditions. J. Fruit. Ornam. Plant Res. 14: 199–210Google Scholar
  36. Velikova V, Loreto F. 2005. On the relationship between isoprene emission and thermo tolerance in Phragmites ausrralis leaves exposed to high temperatures and during the recovery from a heat stress. Plant, Cell Environ. 28: 318–327CrossRefGoogle Scholar
  37. Velikova V, Yordanov I, Edreva A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci. 151: 59–66Google Scholar
  38. Wang CY, Buta JG. 1994. Methyl Jasmonate reduces chilling injury in Cucurbita pepo through its regulation of abscisic acid and polyamine levels. Environ. Exp. Bot. 34: 427–432CrossRefGoogle Scholar
  39. Wang F, Zeng B, Sun Z, Zhu C. 2009. Relationship between proline and Hg+2 — induced oxidative stress in tolerant rice mutant. Arch. Environ. Contam. Toxicol. 56: 723–731PubMedCrossRefGoogle Scholar
  40. Wang SY. 1999. Methyl jasmonate reduces water stress in strawberry. J. Plant Growth Regul. 18: 127–134PubMedCrossRefGoogle Scholar
  41. Wasternack C, Parthier B. 1997. Jasmonate-signaled plant gene expression. Trends Plant Sci. 2: 302–307CrossRefGoogle Scholar
  42. Xing H, Tan L, An L, Zhao Z, Wang S, Zhang C. 2004. Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: Inverse correlation between leaf abscisic acid accumulation and leaf water loss. Plant Growth Regul. 42: 61–68CrossRefGoogle Scholar

Copyright information

© Korean Society of Crop Science and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Siamak Shirani Bidabadi
    • 1
  • Hamidreza Mehri
    • 1
  • Cyrus Ghobadi
    • 1
  • Bahram Baninasab
    • 1
  • Marzieh Afazel
    • 1
  1. 1.Department of Horticulture, College of AgricultureIsfahan University of Technology, IUT IsfahanIsfahanIran

Personalised recommendations