Journal of Crop Science and Biotechnology

, Volume 15, Issue 3, pp 195–203 | Cite as

Genetic analysis of sodium content and Na/K ratio in relation to salinity tolerance in pearl millet Pennisetum glaucum (L.) R. Br.

  • Arjuna Rao Palakollu Venkata
  • Panda Kusuma Kumari
  • Tavva Surya Santosh Mohan Dev
  • Muktinutalapati Venkata Subba RaoEmail author
  • Vangury Manga
Research Article


Genetic analysis of sodium and sodium/potassium (Na/K) ratios in leaf and stem was carried out through diallel analysis involving two tolerant, one moderately tolerant, and two sensitive genotypes as parents. Three-week-old seedlings were subjected to a critical level of salinization (17 decisiemens per meter of electrical conductivity). Leaf and stem sampling (from 5 parents and 20 hybrids arranged in a randomized block design with three replications of 10 each) was done 30 days after salinization when susceptible parents were severely effected. Predominance of non-additive gene action for stem sodium and dominance component for leaf Na/K were noticed while both additive and non-additive components played a significant role for stem Na/K. A single group of genes seems to be operative for these characters. Hybrids produced by crossing the two sensitive parents were tolerant suggesting genetic complementation and involvement of different loci in the two parents for salinity tolerance. The overall dominant nature of tolerance and the additive gene action for these salinity related characters suggested the possibility of breeding pearl millet lines through hybridization and selection to pyramid the favorable genes.

Key words

combining ability diallel analysis genetic variation pearl millet salinity tolerance 



electrical conductivity in decisiemens per meter


general combining ability


Hoagland nutrient solution


sodium to potassium ratio


specific combining ability


variance of rth array


covariance of rth array


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  1. Abubaker, Murtada M. 1977. The inheritance of salt tolerance in Barley. Dissertations abstracts international B. 37: 5911BGoogle Scholar
  2. Ahsan M, Wright D, Virk DS. 1996. Genetic analysis of salt tolerance in spring wheat (Triticum aesitvum L.). Ceral Res. Commun. 24: 353–360Google Scholar
  3. Arjuna Rao PV. 1999. Characterization and genetic analysis of salinity tolerance in some inbreds of pearl millet, Pennisetum glaucum (L.) R. Br. Ph. D Thesis, Andhra University, Visakhapatnam, IndiaGoogle Scholar
  4. Ashraf M, McNeilly T. 1987. Salinity effects on five cultivars/lines of pearl millet (Pennisetum americanum (L.) Leeke). Plant Soil 103: 13–19CrossRefGoogle Scholar
  5. Ashraf M, McNeilly T. 1992. The potential for exploiting variation in salinity tolerance in pearl millet (Pennisetum ameri canum (L.) Leeke). Plant Breed. 108: 234–240CrossRefGoogle Scholar
  6. Azhar FM, McNeilly T. 1988. The genetic basis of variation for salt tolerance in Sorghum bicolor (L.) Moench seedlings. Plant Breed. 101:114–121CrossRefGoogle Scholar
  7. Chopra N, Chopra N. 1993. Relative salt tolerance of pearl millet (Pennisetum glaucum) varieties in Marwar tract of Rajasthan. Ind. J. Agric. Res. 63: 652–654Google Scholar
  8. Clesceri LS, Greenberg AE, Trussell RR. American Public Health Association, Washington, DC 2005Google Scholar
  9. de Wet JMJ, 1987. Pearl millet (Pennisetum glaucum) in Africa and India. Proceedings of the International Pearl millet work shop 7–11, April, 1986, ICRISAT, Patancheru, India, pp. 3–4Google Scholar
  10. Dua RP. 1989. Salinity tolerance in pearl millet. Ind. J. Agric. Res. 23: 9–14Google Scholar
  11. Epstein E. 1985. Salt tolerant crops: Origin, development and prospects of the concept. Plant Soil 89: 87–198CrossRefGoogle Scholar
  12. Falconer DS. 1981. Introduction to Quantitative Genetics, 2nd ed, Longman, LondonGoogle Scholar
  13. Fooland MR. 1996. Genetic analysis of salt tolerance during vegetative growth in tomato L. esculentum Mill. Plant Breed. 115: 245–250CrossRefGoogle Scholar
  14. Fooland MR, Jones RA. 1991. Genetic analysis of salt tolerance during germination in Lycopersicon. Theor. Appl. Genet. 81: 321–326CrossRefGoogle Scholar
  15. Forster BP, Packniyat H, Simpson CG, Handley LL. 1995. Genetic control of salt tolerance in barley. Intl. Atomic Energy Agency Proc. 347–353Google Scholar
  16. Franson MAH. 1989. Standard methods for the examination of water and wastewater. (eds.) Gregorio GB, Senadhira D. 1993. Genetic analysis of salinity tolerance in rice (Oryza sativa L.). Theor. Appl. Genet. 86: 333–338Google Scholar
  17. Griffing JB. 1956. Concept of general and specific combining ability in relation to diallel crossing system. Aust. J. Biol. Sci. 9: 463–493Google Scholar
  18. Gu XY, Yan XL, Zheng SL, Lu YG. 1998. Diallel cross analysis of salt tolerance in rice seedlings. J. South China Agric. Univ. 19: 31–35Google Scholar
  19. Hayman BI. 1954a. The analysis of variance of diallel tables. Biometrics 10: 235–244CrossRefGoogle Scholar
  20. Hayman BI. 1954b. The theory and analysis of diallel crosses. Genetics 39: 789–809PubMedGoogle Scholar
  21. Hesham ASA, Mounir EM. 1996. Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.). Euphytica 91: 89–97CrossRefGoogle Scholar
  22. Hoagland DR, Arnon DI. 1938. Nutrient solutions for hydroponic culture. University of California Agricultural Experimental Station Circular # 347Google Scholar
  23. Jinks JL. 1964. Extra Chromosomal Inheritance. Prentice-Hall, LondonGoogle Scholar
  24. Kathiria KB, Sharma RK. 1994. Diallel cross analysis for tillering ability and kernal weight in bread wheat under normal, sodic and saline sodic soils. Gujarat. Agric. Univ. Res. J. 20: 79–83Google Scholar
  25. Khalifa MA, Rushdi MK, Hassaballa EA, Abdallah MH. 1980. Combining ability and genotype X environmental interaction in some wheat crosses: II. Seawater effects. Faculty of Agriculture, Ain Shams Univ. Res. Bull. No. 1292, pp. 12Google Scholar
  26. Koval VS, Koval SF. 1996. Genetic analysis of salt tolerance in barley. Determining the number of genes. Genetika (Moskva). 32: 1098–1103Google Scholar
  27. Krishnamurthy I, Rachid Serraj, Kedarnath Rai, Tom Hash C, Abdullah J Dakheel. 2007. Identification of pearl millet [Pennisetum glaucum (L.) R. Br.] lines tolerant to soil salinity. Euphytica 158: 179–188CrossRefGoogle Scholar
  28. Mahajan S, Narendra T. 2005. Cold, salinity and drought stresses: An overview, Arch. Biochem. Biophy. 444: 139–158CrossRefGoogle Scholar
  29. Mano Y, Takeda K. 1997. Diallel analysis of salt tolerance at germination and the seedling stage in barley. Breed. Sci. 47: 203–209Google Scholar
  30. Mather K, Jinks JL. 1982. Biometrical Genetics, 3rd edn, Chapman and Hall, LondonGoogle Scholar
  31. Mishra B, Akbar M, Seshu DV, Senadhira D. 1996. Genetics of salinity tolerance and ion uptake in rice. Intl. Rice. Res. Notes 21: 38–39Google Scholar
  32. Moeljopawiro S, Ikehashi H. 1981. Inheritance of salt tolerance in rice. Euphytica 30: 291–300CrossRefGoogle Scholar
  33. Muscolo A, Panuccio MR, Sidari, M. 2003. Effect of salinity on growth, carbohydrate metabolism and nutritive properties of Kikuyu grass (Pennisetum clandestinum Hochst). Plant Sci. 164: 1103–1110CrossRefGoogle Scholar
  34. Powell W, Caligari PDS. 1987. The in vitro genetics of barley (Hordeum vulgare L.) detection and analysis of reciprocal differences for culture response to 2,4-Dichlorophenoxyacetic acid. Heredity 59: 293–299CrossRefGoogle Scholar
  35. Singh RK, Chaudhary BD. 1979. Biometrical Methods in Quantitative Genetic Analysis. Kalyani Pubs, Ludhiana, IndiaGoogle Scholar
  36. Spivakov NS. 1990. Genetic nature and degree of inheritance of salt tolerance in Sorghum. Soviet. Agric. Sci. 4: 25–28Google Scholar
  37. Wang W, Vinocur B, Altman A. 2003. Plant responses to drought, salinity and extreme temperature; towards genetic engineering for stress tolerance. Planta 218: 1–14PubMedCrossRefGoogle Scholar

Copyright information

© Korean Society of Crop Science and Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Arjuna Rao Palakollu Venkata
    • 1
  • Panda Kusuma Kumari
    • 1
  • Tavva Surya Santosh Mohan Dev
    • 1
  • Muktinutalapati Venkata Subba Rao
    • 1
    Email author
  • Vangury Manga
    • 1
  1. 1.Plant Genetics and Biotechnology Laboratory, Department of BotanyAndhra UniversityVisakhapatnamIndia

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