Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America

  • Marshal S. HoyEmail author
  • Carl O. Ostberg
Technical Note


We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus. These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.


Cottus eDNA qPCR Pacific coast 



Funding for this work was provided by Seattle City Light agreement #18WNTAAYD00SCL. Cottus samples were obtained from the University of Washington Burke Museum Fish Collection. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

Supplementary material

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Supplementary material 1 (DOCX 18 kb)


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Copyright information

© This is a U.S. government work and its text is not subject to copyright protection in the United States; however, its text may be subject to foreign copyright protection 2019

Authors and Affiliations

  1. 1.Western Fisheries Research Center, USGSSeattleUSA

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