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Conservation Genetics Resources

, Volume 10, Issue 4, pp 601–604 | Cite as

Complete chloroplast genome of Anemoclema glaucifolium (Ranunculaceae), a vulnerable and threatened species endemic to the Hengduan Mountains

  • Nan Jiang
  • Zhuang Zhou
  • Jun-Bo Yang
  • Wen-Bin YuEmail author
Technical Note

Abstract

Anemoclema glaucifolium is a vulnerable and threatened species endemic to the Hengduan Mountains. In this study, we reported a complete plastome of A. glaucifolium, which was de novo assembled using Next-generation Sequencing data. The plastome of A. glaucifolium was quadripartite and 160,400 bp in size, including two inverted repeats (31,256 bp for each) that separate one large single copy (80,251 bp) and one small single copy (17,637 bp) regions. The plastome contained 113 unique genes (with 80 protein-coding, 4 ribosomal RNA and 29 tRNA genes), and 24 entire genes, and partial rps12 (799 bp), rps36 (3 bp) and ycf1 (1097 bp) duplicated in the IR regions. The trnT-UGU tRNA was lost in A. glaucifolium. A maximum likelihood tree using the plastome data recovered A. glaucifolium sister to Clematis spp.

Keywords

Anemoclema glaucifolium Tribe Anenomeae Chloroplast genome Ranunculaceae Genome rearrangement Clematis 

Notes

Acknowledgements

We are grateful to the Germplasm Bank of Wild Species in Southwest China for supporting NGS; to Jing Yang, Ji-Xiong Yang, and Zhi-Rong Zhang for their assistance in molecular experiments. This study was supported by NSFC (31200158) and NSF of Zhejiang (LQ12C02002).

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Center for Integrative ConservationXishuangbanna Tropical Botanical GardenMenglaChina
  2. 2.Zhejiang Institute of Subtropical CropsZhejiang Academy of Agricultural SciencesWenzhouChina
  3. 3.Germplasm Bank of Wild Species, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  4. 4.Southeast Asia Biodiversity Research InstituteChinese Academy of ScienceNay Pyi TawMyanmar

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