Development of nuclear SNP markers for the timber tracking of the African tree species Sapelli, Entandrophragma cylindricum
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We describe the development of new nuclear SNP markers for the genetic timber tracking of the geographical origin of Sapelli, Entandrophragma cylindricum (Meliaceae). Restriction associated DNA sequencing (RADseq) of two reference individuals yielded 1131 putative SNPs. Among those, 131 were selected to design four MassARRAY multiplexes and screened at 178 individuals. Seventy-two loci were selected for further use in genetic tracking.
KeywordsSingle nucleotide polymorphism Entandrophragma RAD sequencing MassARRAY Timber tracking
We would like to thank Dr. E. Foli (Ghana), Prof. B. Sonké (Cameroon), Prof. J. Loumeto (Congo Brazzaville), C. Botefa (DRC), A. Monkengo no Mpenge (DRC), Prof. C. Amani (DRC) and G. Assimbo (DRC) for their support during the sampling. The work was financially supported by the International Tropical Timber Organization (project PD 620/11 Rev.1). Genotyping was performed at the Genomic and Sequencing Facility of Bordeaux (grants from the Conseil Regional d’Aquitaine n°20030304002FA and 20040305003FA, the European Union, FEDER n°2003227 and Investissements d’avenir, N°ANR-10-EQPX-16-01).