The complete mitochondrial genome of Juema pig (Suina:Suidae)

Technical Note
  • 24 Downloads

Abstract

Juema pig (Suina:Suidae), a rare, miniature and primitive species, has been living and razing on the Qinghai-Tibet plateau completely wild for thousands of years. Owing to the cold plateau climate, low energy, and low carbohydrate diet, Juema pig has some unique genetic characteristics that differentiate it from other pig breeds. If the species should not be protected in a timely manner, it would become extinct in the future. In June 2006, No. 662 announcement of the People’s Republic ofChina has included the Tibetan pigs in the national protection list of poultry genetic resources. Developing reasonable conservation and utilization measures for Juema pig requires deeper understanding of molecular genetics. The complete mitochondrial genome of Juema pig was determined in this study. Similar to other vertebrate, the mitogenome is 16,690 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a D-loop region. The overall base composition of the H-strand is 34.76% for A, 26.17% for C, 13.24% for G and 25.83% for T. To ensure the sequence is reliable, phylogenetic tree was constructed using MEGA 6 with Neighbor-Joiningmethod (NJ) methods, demonstrating that Juema pig was clustered in subfamily Suina.

Keywords

Juema pig Complete mitochondrial genome Phylogenetic tree 

Notes

Funding

The work was supported or partly supported by the National Natural Science Foundation of China (31301976).

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

References

  1. Johns MB Jr, Paulus-Thomas JE (1989) Purification of human genomic DNA from whole blood using sodium perchlorate in place of phenol. Anal Biochem 180:276–278CrossRefPubMedGoogle Scholar
  2. Li F, Ma X, Zhang X, Luo X, Yue Y, Jiang L (2013) Characteristics of blood physiological and biochemical and immunological indexes of Hezuo swine. J Gansu Agric Univ 48:24–27Google Scholar
  3. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evolut 30:2725–2729 (in Chinese)CrossRefGoogle Scholar
  4. Xu D, Li QH, He CQ, Chai YL, Ma HM (2015) The complete mitochondrial genome of the Ningxiang pig. Mitochondr DNA 26:623–624CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Animal Husbandry Station of Gannan Tibetan Autonomous PrefectureHezuoPeople’s Republic of China
  2. 2.Gansu Animal Husbandry Industry AdministrationLanzhouPeople’s Republic of China
  3. 3.Lanzhou Institute of Husbandry and Pharmaceutical SciencesChinese Academy of Agricultural SciencesLanzhouPeople’s Republic of China
  4. 4.Animal Disease Prevention and Control Center of Gannan Tibetan Autonomous PrefectureHezuoPeople’s Republic of China

Personalised recommendations