Development of nuclear SNP markers for genetic tracking of Iroko, Milicia excelsa and Milicia regia
Restriction associated DNA sequencing was conducted on two genetically independent individuals of Iroko, Milicia excelsa, for the discovery of nuclear SNPs. Ninety-four samples, well-distributed over the natural range and including timber DNA, were screened at 138 loci on a MassARRAY iPLEX system. Amplification success was high and 77 loci were selected to design a set of markers for genetic timber tracking purposes.
KeywordsSingle nucleotide polymorphism Milicia RAD sequencing MassARRAY Timber tracking
The work was financially supported by the International Tropical Timber Organization (Project PD 620/11 Rev.1). Genotyping was performed at the Genomic and Sequencing Facility of Bordeaux (Grants from the Conseil Regional d’Aquitaine No. 20030304002FA and 20040305003FA and from the European Union, FEDER No. 2003227 and from Investissements d’avenir, Convention attributive d’aide No. ANR-10-EQPX-16-01).
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