Development of nuclear SNP markers for genetic tracking of Iroko, Milicia excelsa and Milicia regia
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Restriction associated DNA sequencing was conducted on two genetically independent individuals of Iroko, Milicia excelsa, for the discovery of nuclear SNPs. Ninety-four samples, well-distributed over the natural range and including timber DNA, were screened at 138 loci on a MassARRAY iPLEX system. Amplification success was high and 77 loci were selected to design a set of markers for genetic timber tracking purposes.
KeywordsSingle nucleotide polymorphism Milicia RAD sequencing MassARRAY Timber tracking
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