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Conservation Genetics Resources

, Volume 9, Issue 1, pp 67–71 | Cite as

Design and validation of sixteen single nucleotide polymorphism to investigate plastid DNA sequence variation in Noccaea caerulescens (Brassicaceae)

  • Cédric Gonneau
  • Nausicaa Noret
  • Cécile Godé
  • Jonathan Kitt
  • Catherine Sirguey
  • Thibault Sterckeman
  • Marcus A. Koch
  • Maxime Pauwels
Technical Note

Abstract

Noccaea caerulescens :belong to the legally protected vegetation of metalliferous sites in Europe. It is also a model species for the study of metal-related traits. To improve the understanding of the evolutionary history of the species, 32 single nucleotide polymorphism (SNP) were discovered in several intragenic spacers of the chloroplastic genome by Sanger Sequencing from a representative set of populations throughout Europe. From these 32 SNPs 16 SNP were validated by KASPar assay, which provided a cost-effective set of polymorphisms to be used in population genetic and phylogeography studies of this plant species and close relatives.

Keywords

Single nucleotide polymorphism Thlaspi caerulescens KASPar Haplotype Chloroplastic genome 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12686_2016_622_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 18 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Cédric Gonneau
    • 1
    • 2
  • Nausicaa Noret
    • 3
  • Cécile Godé
    • 4
  • Jonathan Kitt
    • 4
  • Catherine Sirguey
    • 1
    • 2
  • Thibault Sterckeman
    • 1
    • 2
  • Marcus A. Koch
    • 5
  • Maxime Pauwels
    • 4
  1. 1.Laboratoire Sols et Environnement, UMR 1120Université de LorraineVandœuvre-lès-Nancy cedexFrance
  2. 2.Laboratoire Sols et Environnement, UMR 1120INRAVandœuvre-lès-Nancy cedexFrance
  3. 3.Laboratoire d’Ecologie Végétale et BiogéochimieUniversité Libre de BruxellesBruxellesBelgium
  4. 4.Laboratoire Evolution Ecologie PaleontologieUniversité Lille Sciences et TechnologiesVilleneuve d’Ascq cedexFrance
  5. 5.Department of Biodiversity and Plant Systematics, Center for Organismal Studies HeidelbergHeidelberg UniversityHeidelbergGermany

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