Design and validation of sixteen single nucleotide polymorphism to investigate plastid DNA sequence variation in Noccaea caerulescens (Brassicaceae)
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Noccaea caerulescens :belong to the legally protected vegetation of metalliferous sites in Europe. It is also a model species for the study of metal-related traits. To improve the understanding of the evolutionary history of the species, 32 single nucleotide polymorphism (SNP) were discovered in several intragenic spacers of the chloroplastic genome by Sanger Sequencing from a representative set of populations throughout Europe. From these 32 SNPs 16 SNP were validated by KASPar assay, which provided a cost-effective set of polymorphisms to be used in population genetic and phylogeography studies of this plant species and close relatives.
KeywordsSingle nucleotide polymorphism Thlaspi caerulescens KASPar Haplotype Chloroplastic genome
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Conflict of interest
The authors declare that they have no conflict of interest.
- Batley J (ed) (2015) SNP genotyping using KASPar assays. Springer, New YorkGoogle Scholar
- Baumbach H (2012) Metallophytes and metallicolous vegetation: evolutionary aspects, taxonomic changes and conservational status in Central Europe. INTECH Open Access Publisher, CroatiaGoogle Scholar
- Ernst W (1974) Schwermetallvegetation der erde. Gustav Fischer Verlag, Stuttgart.Google Scholar
- Meyer F (2006) Kritische Revision der “Thlaspi”-Arten Europas, Afrikas und Vorderasiens. Spezieller Teil. IX. Noccaea Moench. (Critical revision of the European, African and Middle East genus Thlaspi. Special part. 9. Noccaea Moench.). Haussknechtia 12:1–343Google Scholar